S35A3_MOUSE
ID S35A3_MOUSE Reviewed; 326 AA.
AC Q8R1T4; Q8BRW7;
DT 15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=UDP-N-acetylglucosamine transporter;
DE AltName: Full=Golgi UDP-GlcNAc transporter;
DE AltName: Full=Solute carrier family 35 member A3;
GN Name=Slc35a3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Aorta, Embryo, and Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
CC -!- FUNCTION: Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc)
CC transporter in the Golgi apparatus. May supply UDP-GlcNAc as substrate
CC for Golgi-resident glycosyltransferases that generate branching of
CC diantennary oligosaccharides (By similarity).
CC {ECO:0000250|UniProtKB:Q9Y2D2}.
CC -!- SUBUNIT: Interacts with SLC35A2; the interaction is reduced in the
CC presence of SLC35A4 (By similarity). Found in a complex with SLC35A2
CC and SLC35A4 (By similarity). {ECO:0000250|UniProtKB:Q9Y2D2}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q9Y2D2}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A
CC subfamily. {ECO:0000305}.
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DR EMBL; BC024110; AAH24110.1; -; mRNA.
DR EMBL; AK029398; BAC26434.1; -; mRNA.
DR EMBL; AK041195; BAC30858.1; -; mRNA.
DR EMBL; AK049484; BAC33773.1; -; mRNA.
DR CCDS; CCDS17791.1; -.
DR RefSeq; NP_659151.1; NM_144902.3.
DR RefSeq; XP_006501489.1; XM_006501426.3.
DR AlphaFoldDB; Q8R1T4; -.
DR SMR; Q8R1T4; -.
DR STRING; 10090.ENSMUSP00000029569; -.
DR iPTMnet; Q8R1T4; -.
DR PhosphoSitePlus; Q8R1T4; -.
DR jPOST; Q8R1T4; -.
DR MaxQB; Q8R1T4; -.
DR PaxDb; Q8R1T4; -.
DR PRIDE; Q8R1T4; -.
DR ProteomicsDB; 256880; -.
DR Antibodypedia; 19975; 58 antibodies from 19 providers.
DR DNASU; 229782; -.
DR Ensembl; ENSMUST00000029569; ENSMUSP00000029569; ENSMUSG00000027957.
DR Ensembl; ENSMUST00000120120; ENSMUSP00000112674; ENSMUSG00000027957.
DR GeneID; 229782; -.
DR KEGG; mmu:229782; -.
DR UCSC; uc008rcp.1; mouse.
DR CTD; 23443; -.
DR MGI; MGI:1917648; Slc35a3.
DR VEuPathDB; HostDB:ENSMUSG00000027957; -.
DR eggNOG; KOG2234; Eukaryota.
DR GeneTree; ENSGT00950000182827; -.
DR HOGENOM; CLU_024645_1_0_1; -.
DR InParanoid; Q8R1T4; -.
DR OMA; AFFSVTM; -.
DR OrthoDB; 703674at2759; -.
DR PhylomeDB; Q8R1T4; -.
DR TreeFam; TF315345; -.
DR Reactome; R-MMU-727802; Transport of nucleotide sugars.
DR BioGRID-ORCS; 229782; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Slc35a3; mouse.
DR PRO; PR:Q8R1T4; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8R1T4; protein.
DR Bgee; ENSMUSG00000027957; Expressed in left colon and 228 other tissues.
DR ExpressionAtlas; Q8R1T4; baseline and differential.
DR Genevisible; Q8R1T4; MM.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; ISS:UniProtKB.
DR InterPro; IPR007271; Nuc_sug_transpt.
DR PANTHER; PTHR10231; PTHR10231; 1.
DR Pfam; PF04142; Nuc_sug_transp; 1.
DR PIRSF; PIRSF005799; UDP-gal_transpt; 1.
PE 2: Evidence at transcript level;
KW Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..326
FT /note="UDP-N-acetylglucosamine transporter"
FT /id="PRO_0000213358"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 209
FT /note="N -> D (in Ref. 2; BAC30858)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 326 AA; 35976 MW; D9045738EE3701D9 CRC64;
MSANLKYLSL GILVFQTTSL VLTMRYSRTL KEEGPRYLSS TAVVVAEFLK IMACIFLVYK
DSKCSVRALN RVLHDEILNK PMETLKLAIP SGIYTLQNNL LYVALSNLDA ATYQVTYQLK
ILTTALFSVS MLGKKLGVYQ WLSLVILMAG VAFVQWPSDS QELNSKDLST GSQFVGLMAV
LTACFSSGFA GVYFEKILKE TKQSVWIRNI QLGFFGSIFG LMGVYVYDGE LVSKNGFFQG
YNQLTWIVVA LQALGGLVIA AVIKYADNIL KGFATSLSII LSTIISYFWL QDFVPTSVFF
LGAILVIAAT FLYGYDPKPA GNPTKA
