S35A4_HUMAN
ID S35A4_HUMAN Reviewed; 324 AA.
AC Q96G79; A8K013;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Probable UDP-sugar transporter protein SLC35A4 {ECO:0000305};
DE AltName: Full=Solute carrier family 35 member A4 {ECO:0000312|HGNC:HGNC:20753};
GN Name=SLC35A4 {ECO:0000312|HGNC:HGNC:20753};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Adrenal gland;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP ALTERNATIVE INITIATION (ISOFORM 2).
RX PubMed=25621764; DOI=10.7554/elife.03971;
RA Andreev D.E., O'Connor P.B., Fahey C., Kenny E.M., Terenin I.M.,
RA Dmitriev S.E., Cormican P., Morris D.W., Shatsky I.N., Baranov P.V.;
RT "Translation of 5' leaders is pervasive in genes resistant to eIF2
RT repression.";
RL Elife 4:E03971-E03971(2015).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, AND IDENTIFICATION IN A COMPLEX
RP WITH SLC35A2 AND SLC35A3.
RX PubMed=28167211; DOI=10.1016/j.bbamcr.2017.02.002;
RA Sosicka P., Maszczak-Seneczko D., Bazan B., Shauchuk Y., Kaczmarek B.,
RA Olczak M.;
RT "An insight into the orphan nucleotide sugar transporter SLC35A4.";
RL Biochim. Biophys. Acta 1864:825-838(2017).
RN [7]
RP FUNCTION, AND TRANSPORTER ACTIVITY.
RX PubMed=34015330; DOI=10.1016/j.jbc.2021.100789;
RA Ury B., Potelle S., Caligiore F., Whorton M.R., Bommer G.T.;
RT "The promiscuous binding pocket of SLC35A1 ensures redundant transport of
RT CDP-ribitol to the Golgi.";
RL J. Biol. Chem. 296:100789-100789(2021).
CC -!- FUNCTION: Mediates the transport of CDP-ribitol (PubMed:34015330). Does
CC not exhibit CMP-sialic acid, UDP-galactose and UDP-N-acetylglucosamine
CC transport activity (PubMed:28167211, PubMed:34015330).
CC {ECO:0000269|PubMed:28167211, ECO:0000269|PubMed:34015330}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CDP(out) + CDP-L-ribitol(in) = CDP(in) + CDP-L-ribitol(out);
CC Xref=Rhea:RHEA:71579, ChEBI:CHEBI:57608, ChEBI:CHEBI:58069;
CC Evidence={ECO:0000269|PubMed:34015330};
CC -!- SUBUNIT: Found in a complex with SLC35A2 and SLC35A3.
CC {ECO:0000269|PubMed:28167211}.
CC -!- INTERACTION:
CC Q96G79; Q13323: BIK; NbExp=3; IntAct=EBI-12363689, EBI-700794;
CC Q96G79; P21964: COMT; NbExp=3; IntAct=EBI-12363689, EBI-372265;
CC Q96G79; Q7Z7G2: CPLX4; NbExp=3; IntAct=EBI-12363689, EBI-18013275;
CC Q96G79; Q96BA8: CREB3L1; NbExp=3; IntAct=EBI-12363689, EBI-6942903;
CC Q96G79; P00387: CYB5R3; NbExp=3; IntAct=EBI-12363689, EBI-1046040;
CC Q96G79; O00559: EBAG9; NbExp=3; IntAct=EBI-12363689, EBI-8787095;
CC Q96G79; Q15125: EBP; NbExp=3; IntAct=EBI-12363689, EBI-3915253;
CC Q96G79; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-12363689, EBI-781551;
CC Q96G79; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-12363689, EBI-18304435;
CC Q96G79; A2A2Y4: FRMD3; NbExp=3; IntAct=EBI-12363689, EBI-6911547;
CC Q96G79; Q9UJ14: GGT7; NbExp=3; IntAct=EBI-12363689, EBI-1058791;
CC Q96G79; P48165: GJA8; NbExp=3; IntAct=EBI-12363689, EBI-17458373;
CC Q96G79; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-12363689, EBI-13345167;
CC Q96G79; Q9BSE4: HERPUD2; NbExp=3; IntAct=EBI-12363689, EBI-2868124;
CC Q96G79; Q9NY72: SCN3B; NbExp=3; IntAct=EBI-12363689, EBI-17247926;
CC Q96G79; Q96EP9: SLC10A4; NbExp=3; IntAct=EBI-12363689, EBI-17456472;
CC Q96G79; Q9UKG4: SLC13A4; NbExp=3; IntAct=EBI-12363689, EBI-12808018;
CC Q96G79; Q8WWF3: SSMEM1; NbExp=3; IntAct=EBI-12363689, EBI-17280858;
CC Q96G79; Q8N9I0: SYT2; NbExp=3; IntAct=EBI-12363689, EBI-8032987;
CC Q96G79; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-12363689, EBI-8638294;
CC Q96G79; Q6P9G4: TMEM154; NbExp=3; IntAct=EBI-12363689, EBI-13329239;
CC Q96G79; Q96Q45-2: TMEM237; NbExp=5; IntAct=EBI-12363689, EBI-10982110;
CC Q96G79; Q4KMG9: TMEM52B; NbExp=3; IntAct=EBI-12363689, EBI-18178701;
CC Q96G79; Q9BSR8: YIPF4; NbExp=3; IntAct=EBI-12363689, EBI-751253;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:28167211}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1;
CC IsoId=Q96G79-1; Sequence=Displayed;
CC Name=2; Synonyms=uORF {ECO:0000303|PubMed:25621764};
CC IsoId=L0R6Q1-1; Sequence=External;
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A
CC subfamily. {ECO:0000305}.
CC -!- CAUTION: The translation and the expression of this protein may be
CC maintained under stress conditions thanks to the presence of the
CC upstream ORF in transcripts. {ECO:0000269|PubMed:25621764}.
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DR EMBL; AK289378; BAF82067.1; -; mRNA.
DR EMBL; AL833972; CAD38817.1; -; mRNA.
DR EMBL; CH471062; EAW62040.1; -; Genomic_DNA.
DR EMBL; BC009906; AAH09906.1; -; mRNA.
DR CCDS; CCDS4231.1; -. [Q96G79-1]
DR RefSeq; NP_542401.1; NM_080670.3. [Q96G79-1]
DR AlphaFoldDB; Q96G79; -.
DR SMR; Q96G79; -.
DR BioGRID; 125263; 33.
DR IntAct; Q96G79; 26.
DR MINT; Q96G79; -.
DR STRING; 9606.ENSP00000424566; -.
DR TCDB; 2.A.7.12.12; the drug/metabolite transporter (dmt) superfamily.
DR GlyGen; Q96G79; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q96G79; -.
DR PhosphoSitePlus; Q96G79; -.
DR BioMuta; SLC35A4; -.
DR DMDM; 74731793; -.
DR PaxDb; Q96G79; -.
DR PeptideAtlas; Q96G79; -.
DR PRIDE; Q96G79; -.
DR Antibodypedia; 45451; 24 antibodies from 12 providers.
DR DNASU; 113829; -.
DR Ensembl; ENST00000323146.8; ENSP00000327133.3; ENSG00000176087.16. [Q96G79-1]
DR Ensembl; ENST00000514199.1; ENSP00000424566.1; ENSG00000176087.16. [Q96G79-1]
DR Ensembl; ENST00000612662.2; ENSP00000479255.1; ENSG00000176087.16. [Q96G79-1]
DR GeneID; 113829; -.
DR KEGG; hsa:113829; -.
DR MANE-Select; ENST00000323146.8; ENSP00000327133.3; NM_080670.4; NP_542401.1.
DR UCSC; uc003lgg.1; human. [Q96G79-1]
DR CTD; 113829; -.
DR DisGeNET; 113829; -.
DR GeneCards; SLC35A4; -.
DR HGNC; HGNC:20753; SLC35A4.
DR HPA; ENSG00000176087; Low tissue specificity.
DR neXtProt; NX_Q96G79; -.
DR OpenTargets; ENSG00000176087; -.
DR PharmGKB; PA134971555; -.
DR VEuPathDB; HostDB:ENSG00000176087; -.
DR eggNOG; KOG2234; Eukaryota.
DR GeneTree; ENSGT00950000182827; -.
DR HOGENOM; CLU_024645_5_1_1; -.
DR InParanoid; Q96G79; -.
DR OMA; SSCVVMI; -.
DR OrthoDB; 754910at2759; -.
DR PhylomeDB; Q96G79; -.
DR TreeFam; TF315345; -.
DR PathwayCommons; Q96G79; -.
DR SignaLink; Q96G79; -.
DR BioGRID-ORCS; 113829; 11 hits in 1075 CRISPR screens.
DR ChiTaRS; SLC35A4; human.
DR GenomeRNAi; 113829; -.
DR Pharos; Q96G79; Tdark.
DR PRO; PR:Q96G79; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q96G79; protein.
DR Bgee; ENSG00000176087; Expressed in islet of Langerhans and 178 other tissues.
DR ExpressionAtlas; Q96G79; baseline and differential.
DR Genevisible; Q96G79; HS.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0015165; F:pyrimidine nucleotide-sugar transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR InterPro; IPR007271; Nuc_sug_transpt.
DR PANTHER; PTHR10231; PTHR10231; 1.
DR Pfam; PF04142; Nuc_sug_transp; 1.
DR PIRSF; PIRSF005799; UDP-gal_transpt; 1.
PE 1: Evidence at protein level;
KW Alternative initiation; Golgi apparatus; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..324
FT /note="Probable UDP-sugar transporter protein SLC35A4"
FT /id="PRO_0000337748"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28167211"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..52
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:28167211"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..85
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28167211"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..142
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:28167211"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..180
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28167211"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..214
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:28167211"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..250
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28167211"
FT TRANSMEM 251..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..275
FT /note="Lumenal"
FT /evidence="ECO:0000305|PubMed:28167211"
FT TRANSMEM 276..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299..324
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28167211"
FT CONFLICT 79
FT /note="G -> E (in Ref. 1; BAF82067)"
FT /evidence="ECO:0000305"
FT CONFLICT 81
FT /note="P -> L (in Ref. 1; BAF82067)"
FT /evidence="ECO:0000305"
FT CONFLICT 306
FT /note="F -> L (in Ref. 1; BAF82067)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 324 AA; 34593 MW; 9BD54BA459B6C94A CRC64;
MSVEDGGMPG LGRPRQARWT LMLLLSTAMY GAHAPLLALC HVDGRVPFRP SSAVLLTELT
KLLLCAFSLL VGWQAWPQGP PPWRQAAPFA LSALLYGANN NLVIYLQRYM DPSTYQVLSN
LKIGSTAVLY CLCLRHRLSV RQGLALLLLM AAGACYAAGG LQVPGNTLPS PPPAAAASPM
PLHITPLGLL LLILYCLISG LSSVYTELLM KRQRLPLALQ NLFLYTFGVL LNLGLHAGGG
SGPGLLEGFS GWAALVVLSQ ALNGLLMSAV MKHGSSITRL FVVSCSLVVN AVLSAVLLRL
QLTAAFFLAT LLIGLAMRLY YGSR
