ID   BEPC_BRUSU              Reviewed;         456 AA.
AC   Q8G0Y6; G0K9M2;
DT   19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Outer membrane efflux protein BepC;
DE   Flags: Precursor;
GN   Name=bepC; OrderedLocusNames=BR0945, BS1330_I0941;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
RN   [3]
RP   FUNCTION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RC   STRAIN=1330;
RX   PubMed=17088356; DOI=10.1128/iai.01349-06;
RA   Posadas D.M., Martin F.A., Sabio y Garcia J.V., Spera J.M., Delpino M.V.,
RA   Baldi P., Campos E., Cravero S.L., Zorreguieta A.;
RT   "The TolC homologue of Brucella suis is involved in resistance to
RT   antimicrobial compounds and virulence.";
RL   Infect. Immun. 75:379-389(2007).
RN   [4]
RP   SUBUNIT.
RC   STRAIN=1330;
RX   PubMed=19201794; DOI=10.1128/jb.01198-08;
RA   Martin F.A., Posadas D.M., Carrica M.C., Cravero S.L., O'Callaghan D.,
RA   Zorreguieta A.;
RT   "Interplay between two RND systems mediating antimicrobial resistance in
RT   Brucella suis.";
RL   J. Bacteriol. 191:2530-2540(2009).
CC   -!- FUNCTION: Involved in the efflux of toxic and relatively hydrophobic
CC       compounds. Influences survival inside the host.
CC       {ECO:0000269|PubMed:17088356}.
CC   -!- SUBUNIT: Probably part of a tripartite efflux pump, which is composed
CC       of an outer membrane efflux protein, an inner membrane protein and a
CC       protein that expands the periplasmic space. Could form a tripartite
CC       pump with BepD and BepE or with BepF and BepG.
CC       {ECO:0000269|PubMed:17088356, ECO:0000269|PubMed:19201794}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Mutation strongly affects resistance to bile
CC       salts and toxic chemicals, and significantly decreases the resistance
CC       to antibiotics such as erythromycin, ampicillin, tetracycline, and
CC       norfloxacin. The mutant also has attenuated effects in the mouse model
CC       of infection. {ECO:0000269|PubMed:17088356}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
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DR   EMBL; AE014291; AAN29871.1; -; Genomic_DNA.
DR   EMBL; CP002997; AEM18288.1; -; Genomic_DNA.
DR   RefSeq; WP_011068960.1; NZ_KN046804.1.
DR   AlphaFoldDB; Q8G0Y6; -.
DR   SMR; Q8G0Y6; -.
DR   EnsemblBacteria; AEM18288; AEM18288; BS1330_I0941.
DR   GeneID; 45052002; -.
DR   KEGG; bms:BR0945; -.
DR   KEGG; bsi:BS1330_I0941; -.
DR   HOGENOM; CLU_012817_0_1_5; -.
DR   OMA; SQTIWDS; -.
DR   Proteomes; UP000007104; Chromosome I.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR003423; OMP_efflux.
DR   InterPro; IPR010130; T1SS_OMP_TolC.
DR   Pfam; PF02321; OEP; 2.
DR   TIGRFAMs; TIGR01844; type_I_sec_TolC; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell outer membrane; Coiled coil; Membrane; Signal;
KW   Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..456
FT                   /note="Outer membrane efflux protein BepC"
FT                   /id="PRO_0000390647"
FT   COILED          312..341
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   456 AA;  48518 MW;  3CF3CBED5639292B CRC64;
     MRYTVFKACK ELVAAAVLLS GTVLTGQAAL SETLTGALVK AYKNNAPLNS SRAGVRIQDE
     NVAIAKSAYR PQITGSYNIS RGKTPATDYR TTGTVGIQLN QMLFDGFQTR NNVAAAETQV
     FAQRENLRND EQNTLYQAVA AYMDVYQLRQ IAALREKNLA AMNEQVRAAR ARLDVGEGTR
     TDVAQAEASR STAIAALNAA RADVKTAEAT YMQVVGSLPD KLTPASAARH LPQSPSQAYA
     SALASHPGIL ATKYAVNAAG YNVKAKEGAL LPTIGLTASA SQLDTIAGTD MGDGNTASIG
     VGVNIPIYTG GRTSAQIRQS KEQLGQARIE VDVVQDKVRQ AISSAWSQLE AARASVAANR
     DGIAAAQLAL DGVIEERKVG QRTTLDVLNA QNDLVAVQIA LVQAEHDVVV ASYALLNATG
     RMTADQLGLQ VAQYKPEEHY KAVKDKWFGL RTPDGR