S35B2_MOUSE
ID S35B2_MOUSE Reviewed; 431 AA.
AC Q91ZN5; Q3U221; Q8BTH2;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Adenosine 3'-phospho 5'-phosphosulfate transporter 1;
DE AltName: Full=PAPS transporter 1;
DE AltName: Full=Solute carrier family 35 member B2;
GN Name=Slc35b2; Synonyms=J207, Papst1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=BALB/cJ;
RA Yang X., Wang J.-S., Han H., Li R., Wang Y.L.;
RT "Cloning and characterization of a novel gene J207.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Brain, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N, and FVB/N-3; TISSUE=Liver, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Mediates the transport of adenosine 3'-phospho 5'-
CC phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal
CC sulfuryl donor for sulfation events that take place in the Golgi (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q91ZN5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q91ZN5-2; Sequence=VSP_014085;
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC41173.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF414190; AAL07487.1; -; mRNA.
DR EMBL; AK090329; BAC41173.1; ALT_FRAME; mRNA.
DR EMBL; AK144403; BAE25873.1; -; mRNA.
DR EMBL; AK155551; BAE33321.1; -; mRNA.
DR EMBL; BC025875; AAH25875.1; -; mRNA.
DR EMBL; BC036992; AAH36992.1; -; mRNA.
DR CCDS; CCDS37630.2; -. [Q91ZN5-1]
DR RefSeq; XP_006525059.1; XM_006524996.1.
DR AlphaFoldDB; Q91ZN5; -.
DR BioGRID; 216293; 1.
DR STRING; 10090.ENSMUSP00000037834; -.
DR iPTMnet; Q91ZN5; -.
DR PhosphoSitePlus; Q91ZN5; -.
DR SwissPalm; Q91ZN5; -.
DR EPD; Q91ZN5; -.
DR MaxQB; Q91ZN5; -.
DR PaxDb; Q91ZN5; -.
DR PRIDE; Q91ZN5; -.
DR ProteomicsDB; 256881; -. [Q91ZN5-1]
DR ProteomicsDB; 256882; -. [Q91ZN5-2]
DR Antibodypedia; 30649; 101 antibodies from 24 providers.
DR Ensembl; ENSMUST00000041353; ENSMUSP00000037834; ENSMUSG00000037089. [Q91ZN5-2]
DR Ensembl; ENSMUST00000223987; ENSMUSP00000153631; ENSMUSG00000037089. [Q91ZN5-2]
DR Ensembl; ENSMUST00000224905; ENSMUSP00000153367; ENSMUSG00000037089. [Q91ZN5-1]
DR UCSC; uc008cqx.1; mouse. [Q91ZN5-1]
DR MGI; MGI:1921086; Slc35b2.
DR VEuPathDB; HostDB:ENSMUSG00000037089; -.
DR eggNOG; KOG1581; Eukaryota.
DR GeneTree; ENSGT00940000157927; -.
DR HOGENOM; CLU_036019_3_1_1; -.
DR InParanoid; Q91ZN5; -.
DR OMA; CGAIGQV; -.
DR OrthoDB; 820879at2759; -.
DR PhylomeDB; Q91ZN5; -.
DR TreeFam; TF105926; -.
DR Reactome; R-MMU-174362; Transport and synthesis of PAPS.
DR Reactome; R-MMU-727802; Transport of nucleotide sugars.
DR BioGRID-ORCS; 73836; 7 hits in 73 CRISPR screens.
DR ChiTaRS; Slc35b2; mouse.
DR PRO; PR:Q91ZN5; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q91ZN5; protein.
DR Bgee; ENSMUSG00000037089; Expressed in prostate gland ventral lobe and 271 other tissues.
DR ExpressionAtlas; Q91ZN5; baseline and differential.
DR Genevisible; Q91ZN5; MM.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0046964; F:3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity; ISO:MGI.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046963; P:3'-phosphoadenosine 5'-phosphosulfate transport; ISO:MGI.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Golgi apparatus; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..431
FT /note="Adenosine 3'-phospho 5'-phosphosulfate transporter
FT 1"
FT /id="PRO_0000213375"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..49
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_014085"
FT CONFLICT 61
FT /note="R -> K (in Ref. 2; BAC41173)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 431 AA; 47370 MW; 9C83961CF403FCEB CRC64;
MDARWWAVVV LATLPSLGAG GESPEAPPQS WTQLWLFRFL LNVAGYASFM VPGYLLVQYL
RRKNYLETGR GLCFPLVKAC VFGNEPKAPD EVLLAPRTET AESTPSWQVL KLVFCASGLQ
VSYLTWGILQ ERVMTGSYGA TATSPGEHFT DSQFLVLMNR VLALVVAGLY CVLRKQPRHG
APMYRYSFAS LSNVLSSWCQ YEALKFVSFP TQVLAKASKV IPVMMMGKLV SRRSYEHWEY
LTAGLISIGV SMFLLSSGPE PRSSPATTLS GLVLLAGYIA FDSFTSNWQD ALFAYKMSSV
QMMFGVNLFS CLFTVGSLLE QGALLEGARF MGRHSEFALH ALLLSICSAF GQLFIFYTIG
QFGAAVFTII MTLRQAIAIL LSCLLYGHTV TVVGGLGVAV VFTALLLRVY ARGRKQRGKK
AVPTEPPVQK V
