ID   BEPD_BRUSU              Reviewed;         397 AA.
AC   Q8G2M7; G0K647;
DT   19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Efflux pump periplasmic linker BepD;
DE   Flags: Precursor;
GN   Name=bepD; OrderedLocusNames=BR0291, BS1330_I0292;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
RN   [3]
RP   FUNCTION IN RESISTANCE, SUBUNIT, AND INDUCTION.
RC   STRAIN=1330;
RX   PubMed=19201794; DOI=10.1128/jb.01198-08;
RA   Martin F.A., Posadas D.M., Carrica M.C., Cravero S.L., O'Callaghan D.,
RA   Zorreguieta A.;
RT   "Interplay between two RND systems mediating antimicrobial resistance in
RT   Brucella suis.";
RL   J. Bacteriol. 191:2530-2540(2009).
CC   -!- FUNCTION: Involved in resistance to several unrelated toxic compounds,
CC       such as dyes, detergents and antibiotics.
CC       {ECO:0000269|PubMed:19201794}.
CC   -!- SUBUNIT: Probably part of a tripartite efflux pump, which is composed
CC       of an outer membrane efflux protein, an inner membrane protein and a
CC       protein that expands the periplasmic space. Could form a tripartite
CC       pump with BepC and BepE. {ECO:0000269|PubMed:19201794}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- INDUCTION: Repressed by BepR. {ECO:0000305|PubMed:19201794}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; AE014291; AAN29240.1; -; Genomic_DNA.
DR   EMBL; CP002997; AEM17653.1; -; Genomic_DNA.
DR   RefSeq; WP_006189804.1; NZ_KN046804.1.
DR   AlphaFoldDB; Q8G2M7; -.
DR   SMR; Q8G2M7; -.
DR   EnsemblBacteria; AEM17653; AEM17653; BS1330_I0292.
DR   GeneID; 45051424; -.
DR   KEGG; bms:BR0291; -.
DR   KEGG; bsi:BS1330_I0292; -.
DR   PATRIC; fig|204722.21.peg.1777; -.
DR   HOGENOM; CLU_018816_2_1_5; -.
DR   OMA; RHFEEGQ; -.
DR   PhylomeDB; Q8G2M7; -.
DR   Proteomes; UP000007104; Chromosome I.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Coiled coil; Periplasm; Signal; Transport.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..397
FT                   /note="Efflux pump periplasmic linker BepD"
FT                   /id="PRO_0000390648"
FT   COILED          98..139
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   397 AA;  42672 MW;  4AF7CFBB9B7E98C8 CRC64;
     MTLNRTIRCF AAGAAFIVFA AQPALAQAPG GATPPPPQVF VVDIKPHDVP VTYEYAARIN
     AYRNVQVRAR VGGILLHRNF VEGTQVKAGE VLFEIDPAPY QAELEKAQAQ VAQAEAQYQQ
     SIRDAERAEQ LVQQKVQSAA VRDSAFATRD LNKAAVAAAK AQLRTAELNL SYTKVTAPIS
     GITSQEQVNE GSLIGTDASS SLLTSVTQLD PVYVNFSFTD TEAAEIAKLR AERGATGEDA
     DRLKIKILFG DGKAYDHEGT IDFTSSSLDT ETGTLGVRAV VENPNHRLIP GQFVRAEILD
     IQVKDAITVP KAALMQSAQG QFVYVVNKDN VVEVRPVTGA RELKNDWLIS QGLNSGDRVI
     TEGVIKAVPG RPVQPVVQGV DDKAQAEAGK EQAADKK