S35B3_DROME
ID S35B3_DROME Reviewed; 396 AA.
AC Q9VVD9; Q0PCZ5; Q8T012;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Adenosine 3'-phospho 5'-phosphosulfate transporter 2;
DE AltName: Full=PAPS transporter 2;
DE AltName: Full=Solute carrier family 35 member B3 homolog;
DE AltName: Full=dPAPST2;
GN Name=Papst2; ORFNames=CG7853;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=16873373; DOI=10.1074/jbc.m605045200;
RA Goda E., Kamiyama S., Uno T., Yoshida H., Ueyama M., Kinoshita-Toyoda A.,
RA Toyoda H., Ueda R., Nishihara S.;
RT "Identification and characterization of a novel Drosophila 3'-
RT phosphoadenosine 5'-phosphosulfate transporter.";
RL J. Biol. Chem. 281:28508-28517(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37 AND SER-40, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Mediates the transport of adenosine 3'-phospho 5'-
CC phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal
CC sulfuryl donor for sulfation events that take place in the Golgi.
CC Essential for viability. Involved in glycosaminoglycan synthesis and
CC the subsequent signaling. May be involved in hh and dpp signaling by
CC controlling the sulfation of heparan sulfate (HS).
CC {ECO:0000269|PubMed:16873373}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=2.3 uM for PAPS {ECO:0000269|PubMed:16873373};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:16873373}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16873373}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. Also
CC expressed at lower levels later in embryonic development, and in larvae
CC and adults. {ECO:0000269|PubMed:16873373}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B
CC subfamily. {ECO:0000305}.
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DR EMBL; AB242867; BAF02844.1; -; mRNA.
DR EMBL; AE014296; AAF49373.2; -; Genomic_DNA.
DR EMBL; AY069640; AAL39785.1; -; mRNA.
DR RefSeq; NP_648954.1; NM_140697.4.
DR AlphaFoldDB; Q9VVD9; -.
DR SMR; Q9VVD9; -.
DR BioGRID; 65206; 1.
DR STRING; 7227.FBpp0075039; -.
DR GlyGen; Q9VVD9; 1 site.
DR iPTMnet; Q9VVD9; -.
DR PaxDb; Q9VVD9; -.
DR PRIDE; Q9VVD9; -.
DR DNASU; 39914; -.
DR EnsemblMetazoa; FBtr0075279; FBpp0075039; FBgn0036695.
DR GeneID; 39914; -.
DR KEGG; dme:Dmel_CG7853; -.
DR UCSC; CG7853-RA; d. melanogaster.
DR CTD; 39914; -.
DR FlyBase; FBgn0036695; Papst2.
DR VEuPathDB; VectorBase:FBgn0036695; -.
DR eggNOG; KOG1582; Eukaryota.
DR GeneTree; ENSGT00940000157040; -.
DR HOGENOM; CLU_036019_2_0_1; -.
DR InParanoid; Q9VVD9; -.
DR OMA; QFGYYIG; -.
DR OrthoDB; 1116466at2759; -.
DR PhylomeDB; Q9VVD9; -.
DR Reactome; R-DME-174362; Transport and synthesis of PAPS.
DR Reactome; R-DME-727802; Transport of nucleotide sugars.
DR SABIO-RK; Q9VVD9; -.
DR BioGRID-ORCS; 39914; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 39914; -.
DR PRO; PR:Q9VVD9; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036695; Expressed in oviduct (Drosophila) and 25 other tissues.
DR Genevisible; Q9VVD9; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0000137; C:Golgi cis cisterna; IDA:FlyBase.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0046964; F:3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046963; P:3'-phosphoadenosine 5'-phosphosulfate transport; IDA:UniProtKB.
DR GO; GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IMP:FlyBase.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Glycoprotein; Golgi apparatus; Membrane;
KW Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..396
FT /note="Adenosine 3'-phospho 5'-phosphosulfate transporter
FT 2"
FT /id="PRO_0000213384"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 22..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 37
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 40
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CARBOHYD 57
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 396 AA; 43883 MW; A12968233747E26B CRC64;
MYIDTSASSR MTDNKGSVIT INGGESAGNS PPSQRKSSTS ESPPELRILC FDLTYYNRTT
QFLLSCAGVF FLYILYGYLQ ELIFTVEGFK PYGWFLTLVQ FGYYIGFGLV ERRLEGYRIS
GGSFWNIEPE PRCIPMRTYL ILAALTLGTM GLSNSSLGYL NYPTQVIFKC CKLIPVLVGS
ILIQGKRYGL LDFAAATCMC IGLAWFTLAD SQMTPNFNLL GVAMISGALL CDAAIGNVQE
KAMREFKAPS SEVVFYSYGL GFVYLFVIML VTGNFFSGFA FCLEHPVETF GYGFLFSLSG
YLGIQFVLAL VRSSGAPIAA TVTTARKAVT IAFSFVLFSK PFTLQYLWSG LIVVLGIYLN
VYSKRNKLTL ADVRQRIKQF GAKVARSPSR KFLIEV
