S35B3_MOUSE
ID S35B3_MOUSE Reviewed; 369 AA.
AC Q922Q5;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Adenosine 3'-phospho 5'-phosphosulfate transporter 2;
DE AltName: Full=PAPS transporter 2;
DE AltName: Full=Solute carrier family 35 member B3;
GN Name=Slc35b3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Mediates the transport of adenosine 3'-phospho 5'-
CC phosphosulfate (PAPS), from cytosol into Golgi. PAPS is a universal
CC sulfuryl donor for sulfation events that take place in the Golgi.
CC Compensates for the insufficient expression of SLC35B2/PAPST1 during
CC the synthesis of sulfated glycoconjugates in the colon (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B
CC subfamily. {ECO:0000305}.
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DR EMBL; AK076592; BAC36405.1; -; mRNA.
DR EMBL; AK077208; BAC36684.1; -; mRNA.
DR EMBL; AK141280; BAE24633.1; -; mRNA.
DR EMBL; BC006881; AAH06881.1; -; mRNA.
DR CCDS; CCDS26466.1; -.
DR RefSeq; NP_001163901.1; NM_001170430.1.
DR RefSeq; NP_001163902.1; NM_001170431.1.
DR RefSeq; NP_598821.1; NM_134060.4.
DR RefSeq; XP_017170845.1; XM_017315356.1.
DR AlphaFoldDB; Q922Q5; -.
DR SMR; Q922Q5; -.
DR STRING; 10090.ENSMUSP00000021870; -.
DR GlyGen; Q922Q5; 2 sites.
DR iPTMnet; Q922Q5; -.
DR PhosphoSitePlus; Q922Q5; -.
DR PaxDb; Q922Q5; -.
DR PRIDE; Q922Q5; -.
DR ProteomicsDB; 256556; -.
DR Antibodypedia; 24732; 9 antibodies from 5 providers.
DR DNASU; 108652; -.
DR Ensembl; ENSMUST00000167513; ENSMUSP00000126016; ENSMUSG00000021432.
DR Ensembl; ENSMUST00000225432; ENSMUSP00000153046; ENSMUSG00000021432.
DR GeneID; 108652; -.
DR KEGG; mmu:108652; -.
DR UCSC; uc007qdy.2; mouse.
DR CTD; 51000; -.
DR MGI; MGI:1913978; Slc35b3.
DR VEuPathDB; HostDB:ENSMUSG00000021432; -.
DR eggNOG; KOG1582; Eukaryota.
DR GeneTree; ENSGT00940000157040; -.
DR HOGENOM; CLU_036019_2_0_1; -.
DR InParanoid; Q922Q5; -.
DR OMA; QFGYYIG; -.
DR OrthoDB; 1116466at2759; -.
DR PhylomeDB; Q922Q5; -.
DR Reactome; R-MMU-174362; Transport and synthesis of PAPS.
DR Reactome; R-MMU-727802; Transport of nucleotide sugars.
DR BioGRID-ORCS; 108652; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Slc35b3; mouse.
DR PRO; PR:Q922Q5; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q922Q5; protein.
DR Bgee; ENSMUSG00000021432; Expressed in renal medulla collecting duct and 245 other tissues.
DR ExpressionAtlas; Q922Q5; baseline and differential.
DR Genevisible; Q922Q5; MM.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0046964; F:3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Golgi apparatus; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..369
FT /note="Adenosine 3'-phospho 5'-phosphosulfate transporter
FT 2"
FT /id="PRO_0000213380"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 222
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 369 AA; 40889 MW; F7B617947AD6476D CRC64;
MDLKFNNSRK YVSISVPSKT QAMSPHIKSV EDVVVLGVNL SKFSKLTQFL ICVAGVFVFY
LIYGYLQELI FSVEGFKPYG WYLTLVQFAF YSVFGLIELQ LTQDRRRRIP GKTYMLIAFL
TVGTMGLSNT SLGYLNYPTQ VIFKCCKLIP VMLGGVFIQG KRYNLADVSA AVCMSLGLIW
FTLADSTIAP NFNLTGVMLI SLALCADAVI GNVQEKAMKL HNASNSEMVL YSYSIGFVYI
LLGLSCTSGL GPAVAFCSKN PVGTYGYAFL FSLTGYFGIS FVLALIKIFG ALLAVTVTTG
RKAMTVVLSF LFFAKPFTFQ YIWSGLLVVL GIFLNVYSKN MDKIRLPSVY NMIKKAMDMK
KSRTLAQTV
