BEPE_BRUSU
ID BEPE_BRUSU Reviewed; 1051 AA.
AC Q8G2M6; G0K648;
DT 19-JAN-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Efflux pump membrane transporter BepE;
GN Name=bepE; OrderedLocusNames=BR0292, BS1330_I0293;
OS Brucella suis biovar 1 (strain 1330).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=204722;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=12271122; DOI=10.1073/pnas.192319099;
RA Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT "The Brucella suis genome reveals fundamental similarities between animal
RT and plant pathogens and symbionts.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=22038969; DOI=10.1128/jb.06181-11;
RA Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT "Revised genome sequence of Brucella suis 1330.";
RL J. Bacteriol. 193:6410-6410(2011).
RN [3]
RP FUNCTION IN RESISTANCE, SUBUNIT, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=1330;
RX PubMed=19201794; DOI=10.1128/jb.01198-08;
RA Martin F.A., Posadas D.M., Carrica M.C., Cravero S.L., O'Callaghan D.,
RA Zorreguieta A.;
RT "Interplay between two RND systems mediating antimicrobial resistance in
RT Brucella suis.";
RL J. Bacteriol. 191:2530-2540(2009).
CC -!- FUNCTION: Involved in resistance to several unrelated toxic compounds,
CC such as dyes, detergents and antibiotics.
CC {ECO:0000269|PubMed:19201794}.
CC -!- SUBUNIT: Probably part of a tripartite efflux pump, which is composed
CC of an outer membrane efflux protein, an inner membrane protein and a
CC protein that expands the periplasmic space. Could form a tripartite
CC pump with BepC and BepD. {ECO:0000269|PubMed:19201794}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: Repressed by BepR. {ECO:0000305|PubMed:19201794}.
CC -!- DISRUPTION PHENOTYPE: Shows increased sensitivity to deoxycholate,
CC ethidium bromide and crystal violet. {ECO:0000269|PubMed:19201794}.
CC -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC (TC 2.A.6) family. {ECO:0000305}.
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DR EMBL; AE014291; AAN29241.1; -; Genomic_DNA.
DR EMBL; CP002997; AEM17654.1; -; Genomic_DNA.
DR RefSeq; WP_006189806.1; NZ_KN046804.1.
DR AlphaFoldDB; Q8G2M6; -.
DR SMR; Q8G2M6; -.
DR EnsemblBacteria; AEM17654; AEM17654; BS1330_I0293.
DR GeneID; 45051425; -.
DR KEGG; bms:BR0292; -.
DR KEGG; bsi:BS1330_I0293; -.
DR PATRIC; fig|204722.21.peg.1778; -.
DR HOGENOM; CLU_002755_1_1_5; -.
DR OMA; FRYNEMG; -.
DR PhylomeDB; Q8G2M6; -.
DR Proteomes; UP000007104; Chromosome I.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR Gene3D; 3.30.2090.10; -; 2.
DR InterPro; IPR027463; AcrB_DN_DC_subdom.
DR InterPro; IPR001036; Acrflvin-R.
DR InterPro; IPR004764; HAE1.
DR PANTHER; PTHR32063; PTHR32063; 1.
DR Pfam; PF00873; ACR_tran; 1.
DR PRINTS; PR00702; ACRIFLAVINRP.
DR SUPFAM; SSF82714; SSF82714; 2.
DR TIGRFAMs; TIGR00915; 2A0602; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1051
FT /note="Efflux pump membrane transporter BepE"
FT /id="PRO_0000390650"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 537..557
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 867..887
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 892..912
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 928..948
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 971..991
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1002..1022
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1032..1051
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1051 AA; 113118 MW; BBFBF40D4E4AB70A CRC64;
MNRFFVDRPV FAAVISIVLV LAGLICIRIL PVAQYPELTP PQVVVSATYP GASAETVAQT
VAAPLEQQIN GVENMLYMQS SSLGSGTMQL TVTFALGTDP DQATINVNNR VQRATSSLPQ
EVQRLGVTVD KRFTTILGMV AMFATTDRYD RTYVGNYALL NVVDDLKRLP GVGDVQLLGN
IDYSMRVWLR PDKLAQYNLT PSDVSAAIQE QNAQFAAGRF GDQPDPHAGP FTYTATTQGR
LPDAAAFENI ILRSSSQNAA TLRLKDVARV ELGTESYLVD SNLNGTPAVP IAIYLQPGAN
ALNTMELIQN RMNELKASFP AGIDYAIPFD TTKFIKVSIE EVVHTFIEAI ILVVLVVFIF
LQNWRATLIP VIAVPISIIG TFAGMYVLGF SINLLTLFGL VLAIGIVVDD AIVVLENVER
IMTTEKLSPR KAAIKAMGEV TGPVIAIVLV LCAVFIPVAF MGGLVGEMYK QFAVTIAISV
TLSGLVALTL TPALCALILK PGHHEPILPF RIFNRAFERV TSGYTRGVRF FLKRATIGLI
IFAGLLGSTY YLFERVPGSL LPDEDQGFLF GVAVLPPAAS LERTTVVLDQ VSENIRKNPA
VDNVFAVSGF DLLSGGLKTS AGTMFIMLKD WKERTTPDAD ARNLPRTIMG MNAGIKDGMV
LAFNPPPIMG LSTTGGFELY VQDRTGGGVE SLTQATKLIT EAAAKRPELQ GVRTTFDPNV
PQYDIQLDRE KAKAMGVPIN SVFTAMQATF GSLYVNDFTL YGRNYQVNLQ SEAEFRRDPG
DLKHVFVRAD SGSMIPLDAL VTVKRIVGPD QLERFNAFNA AKVTGNPAPG YTSGDAIKAM
QEVAAQVLPQ GYQIAWTGSA YQEVSTSGTG SQAMIFGLIM VFLILAAQYE RWSLPLAVIT
AVPFAIFGAL LATDLRGLTN DVYFQIGLVT LIGLAAKNAI LIVEFAVLER ESGKSAIEAA
ASAARLRFRP IVMTSLAFIL GVVPLAVSTG AGSASRHSIG TGVIGGMLAA TFIATFFIPM
FYSLIARKPP KKHEDETADT PETPGGTGGG I
