S35B4_DROME
ID S35B4_DROME Reviewed; 352 AA.
AC Q9W429; Q5BIK7;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=UDP-xylose and UDP-N-acetylglucosamine transporter;
DE AltName: Full=ER GDP-fucose transporter;
DE AltName: Full=Solute carrier family 35 member B4 homolog;
GN Name=Efr; ORFNames=CG3774;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND MOTIF.
RX PubMed=19948734; DOI=10.1074/jbc.m109.016964;
RA Ishikawa H.O., Ayukawa T., Nakayama M., Higashi S., Kamiyama S.,
RA Nishihara S., Aoki K., Ishida N., Sanai Y., Matsuno K.;
RT "Two pathways for importing GDP-fucose into the endoplasmic reticulum lumen
RT function redundantly in the O-fucosylation of Notch in Drosophila.";
RL J. Biol. Chem. 285:4122-4129(2010).
CC -!- FUNCTION: Sugar transporter that specifically mediates the transport of
CC UDP-N-acetylglucosamine (UDP-GlcNAc), GDP-fucose and UDP-xylose.
CC Functions redundantly with nac in the O-fucosylation of Notch,
CC positively regulating Notch signaling. Involved in the biosynthesis of
CC heparan sulfate-glycosaminoglycan (HS-GAG) and in Dpp signaling in the
CC wing imaginal disk. {ECO:0000269|PubMed:19948734}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:19948734}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19948734}.
CC -!- DEVELOPMENTAL STAGE: Expressed maternally and zygotically.
CC {ECO:0000269|PubMed:19948734}.
CC -!- DISRUPTION PHENOTYPE: Homozygous lethal. In a weak loss-of-function
CC phenotype, a gap in the fifth longitudinal vein of the adult wing is
CC observed. {ECO:0000269|PubMed:19948734}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014298; AAF46131.1; -; Genomic_DNA.
DR EMBL; BT021217; AAX33365.1; -; mRNA.
DR RefSeq; NP_572299.1; NM_132071.3.
DR RefSeq; NP_727071.1; NM_167062.3.
DR AlphaFoldDB; Q9W429; -.
DR IntAct; Q9W429; 1.
DR STRING; 7227.FBpp0070839; -.
DR PaxDb; Q9W429; -.
DR EnsemblMetazoa; FBtr0070874; FBpp0070839; FBgn0029849.
DR EnsemblMetazoa; FBtr0070875; FBpp0070840; FBgn0029849.
DR GeneID; 31553; -.
DR KEGG; dme:Dmel_CG3774; -.
DR UCSC; CG3774-RA; d. melanogaster.
DR CTD; 31553; -.
DR FlyBase; FBgn0029849; Efr.
DR VEuPathDB; VectorBase:FBgn0029849; -.
DR eggNOG; KOG1583; Eukaryota.
DR GeneTree; ENSGT00390000002915; -.
DR HOGENOM; CLU_033007_1_0_1; -.
DR InParanoid; Q9W429; -.
DR OMA; NYAFDFN; -.
DR OrthoDB; 1209974at2759; -.
DR PhylomeDB; Q9W429; -.
DR Reactome; R-DME-727802; Transport of nucleotide sugars.
DR BioGRID-ORCS; 31553; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31553; -.
DR PRO; PR:Q9W429; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0029849; Expressed in oviduct (Drosophila) and 26 other tissues.
DR ExpressionAtlas; Q9W429; baseline and differential.
DR Genevisible; Q9W429; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:FlyBase.
DR GO; GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0005457; F:GDP-fucose transmembrane transporter activity; IDA:FlyBase.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005462; F:UDP-N-acetylglucosamine transmembrane transporter activity; IDA:FlyBase.
DR GO; GO:0005464; F:UDP-xylose transmembrane transporter activity; IDA:FlyBase.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0036084; P:GDP-fucose import into endoplasmic reticulum lumen; IC:FlyBase.
DR GO; GO:0015783; P:GDP-fucose transmembrane transport; IDA:FlyBase.
DR GO; GO:0015012; P:heparan sulfate proteoglycan biosynthetic process; IDA:FlyBase.
DR GO; GO:0036066; P:protein O-linked fucosylation; IDA:FlyBase.
DR GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; IDA:FlyBase.
DR GO; GO:0015790; P:UDP-xylose transmembrane transport; IDA:FlyBase.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..352
FT /note="UDP-xylose and UDP-N-acetylglucosamine transporter"
FT /id="PRO_0000213390"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 350..352
FT /note="Prevents secretion from ER"
FT CONFLICT 178
FT /note="L -> Q (in Ref. 3; AAX33365)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 352 AA; 39494 MW; 61E36A3819ECFF53 CRC64;
MALNLKALLG MLFVFIGCCS NVVFLELIIQ IDPGAGNLIT FAQFLFIALE GLVFTSKFFT
VRPKIALKDY VILVALFFGA NVCNNYAFNF NIPMPLHMIF RSGSLMANMI MGIVLLKKRY
NLRQYSSVAM ITAGIILCTL VSSGDVKDNT HHSLKVDTSY SDFFWWTVGI GLLTIALLVT
AYMGIYQEVI YKKYGKHPSE ALFFTHMLPL PGFLIMAGNI VQHFGIAWSS EPVAVPLLGA
IGLEWKFPLM LFYLLCNVVT QYVCISAVYV LTTECASLTV TLVVTLRKFV SLLFSIIYFR
NPFTLNHWVG TILVFFGTIL FANVINQVRD AYRARSSRKT HFDTAPLAKK VE
