S35B4_MOUSE
ID S35B4_MOUSE Reviewed; 331 AA.
AC Q8CIA5; Q3TQU7; Q5U681; Q9JJF7;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=UDP-xylose and UDP-N-acetylglucosamine transporter;
DE AltName: Full=Solute carrier family 35 member B4;
GN Name=Slc35b4; ORFNames=MNCb-4414;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.;
RT "Isolation of full-length cDNA clones from mouse brain cDNA library made by
RT oligo-capping method.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Diencephalon, Egg, Spinal cord, Testis, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Sugar transporter that specifically mediates the transport of
CC UDP-xylose (UDP-Xyl) and UDP-N-acetylglucosamine (UDP-GlcNAc) from
CC cytosol into Golgi. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CIA5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CIA5-2; Sequence=VSP_016203;
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35B
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB041549; BAA95034.1; -; mRNA.
DR EMBL; AK029969; BAC26707.1; -; mRNA.
DR EMBL; AK033913; BAC28512.1; -; mRNA.
DR EMBL; AK042150; BAC31186.1; -; mRNA.
DR EMBL; AK138591; BAE23707.1; -; mRNA.
DR EMBL; AK163299; BAE37285.1; -; mRNA.
DR EMBL; BC033512; AAH33512.1; -; mRNA.
DR CCDS; CCDS19989.1; -. [Q8CIA5-1]
DR RefSeq; NP_067410.1; NM_021435.3. [Q8CIA5-1]
DR AlphaFoldDB; Q8CIA5; -.
DR SMR; Q8CIA5; -.
DR STRING; 10090.ENSMUSP00000019143; -.
DR PhosphoSitePlus; Q8CIA5; -.
DR PaxDb; Q8CIA5; -.
DR PeptideAtlas; Q8CIA5; -.
DR PRIDE; Q8CIA5; -.
DR ProteomicsDB; 256883; -. [Q8CIA5-1]
DR ProteomicsDB; 256884; -. [Q8CIA5-2]
DR Antibodypedia; 53731; 39 antibodies from 12 providers.
DR DNASU; 58246; -.
DR Ensembl; ENSMUST00000019143; ENSMUSP00000019143; ENSMUSG00000018999. [Q8CIA5-1]
DR GeneID; 58246; -.
DR KEGG; mmu:58246; -.
DR UCSC; uc009bgw.1; mouse. [Q8CIA5-2]
DR UCSC; uc009bgx.1; mouse. [Q8CIA5-1]
DR CTD; 84912; -.
DR MGI; MGI:1931249; Slc35b4.
DR VEuPathDB; HostDB:ENSMUSG00000018999; -.
DR eggNOG; KOG1583; Eukaryota.
DR GeneTree; ENSGT00390000002915; -.
DR HOGENOM; CLU_033007_1_0_1; -.
DR InParanoid; Q8CIA5; -.
DR OMA; NYAFDFN; -.
DR OrthoDB; 1209974at2759; -.
DR PhylomeDB; Q8CIA5; -.
DR TreeFam; TF312926; -.
DR Reactome; R-MMU-727802; Transport of nucleotide sugars.
DR BioGRID-ORCS; 58246; 4 hits in 71 CRISPR screens.
DR PRO; PR:Q8CIA5; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8CIA5; protein.
DR Bgee; ENSMUSG00000018999; Expressed in ear vesicle and 261 other tissues.
DR ExpressionAtlas; Q8CIA5; baseline and differential.
DR Genevisible; Q8CIA5; MM.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005462; F:UDP-N-acetylglucosamine transmembrane transporter activity; ISO:MGI.
DR GO; GO:0005464; F:UDP-xylose transmembrane transporter activity; ISO:MGI.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0006111; P:regulation of gluconeogenesis; IMP:MGI.
DR GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; ISO:MGI.
DR GO; GO:0015790; P:UDP-xylose transmembrane transport; ISO:MGI.
DR InterPro; IPR013657; UAA.
DR PANTHER; PTHR10778; PTHR10778; 1.
DR Pfam; PF08449; UAA; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Golgi apparatus; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..331
FT /note="UDP-xylose and UDP-N-acetylglucosamine transporter"
FT /id="PRO_0000213387"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..172
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_016203"
FT CONFLICT 238
FT /note="V -> I (in Ref. 3; AAH33512)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 331 AA; 37517 MW; 72AA645C083C8BDA CRC64;
MRPAFAVGLV FAGCCSNVIF LELLARTHPG CGNIVTFAQF LFIAVEGFLF EANLGRKPPA
IPIRYYAIMV TMFFTVSVVN NYALNLNIAM PLHMIFRSGS LIANMILGII ILKKRYSMFK
YTSIALVSAG IFICTFMSAK QVTVQTGLSD KDGFQAFAWW LLGIAALTFA LLMSARMGIF
QETLYRQFGK HSKEALFYNH ALPLPGFIFL ASDIYDHVVL FNKSELYQVP VIGVTMPVMW
FYLLMNVVTQ YVCIRGVFIL TTECTSLTVT LVVTLRKFVS LIFSILYFQN QFTMWHWLGT
SFVFIGTLMY TEVWKNLGTT KSELQKDDKK D
