S35D2_MOUSE
ID S35D2_MOUSE Reviewed; 326 AA.
AC Q762D5; Q3TXR0; Q762D4; Q811G6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose transporter;
DE AltName: Full=Solute carrier family 35 member D2;
DE AltName: Full=UDP-galactose transporter-related protein 8;
DE Short=UGTrel8;
GN Name=Slc35d2; Synonyms=Ugtrel8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA Hiraoka S., Ogawa M., Koseki H.;
RT "Targeted inactivation of the mouse UDP-glucuronic acid/UDP-N-
RT acetylgalactosamine transporter gene.";
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J, and NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Antiporter transporting nucleotide sugars such as UDP-N-
CC acetylglucosamine (UDP-GlcNAc), UDP-glucose (UDP-Glc) and GDP-mannose
CC (GDP-Man) pooled in the cytosol into the lumen of the Golgi in exchange
CC for the corresponding nucleosides monophosphates (UMP for UDP-sugars
CC and GMP for GDP-sugars). May take part in heparan sulfate synthesis by
CC supplying UDP-Glc-NAc, the donor substrate, and thus be involved in
CC growth factor signaling (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q762D5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q762D5-2; Sequence=VSP_030007, VSP_030008;
CC -!- SIMILARITY: Belongs to the TPT transporter family. SLC35D subfamily.
CC {ECO:0000305}.
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DR EMBL; AB117931; BAD18884.1; -; mRNA.
DR EMBL; AB117932; BAD18885.1; -; mRNA.
DR EMBL; AK154257; BAE32469.1; -; mRNA.
DR EMBL; AK159148; BAE34855.1; -; mRNA.
DR EMBL; BC046402; AAH46402.1; -; mRNA.
DR EMBL; BC107211; AAI07212.1; -; mRNA.
DR CCDS; CCDS26595.1; -. [Q762D5-1]
DR RefSeq; NP_001001321.2; NM_001001321.3.
DR AlphaFoldDB; Q762D5; -.
DR SMR; Q762D5; -.
DR STRING; 10090.ENSMUSP00000097040; -.
DR MaxQB; Q762D5; -.
DR PaxDb; Q762D5; -.
DR PRIDE; Q762D5; -.
DR ProteomicsDB; 256675; -. [Q762D5-1]
DR ProteomicsDB; 256676; -. [Q762D5-2]
DR DNASU; 70484; -.
DR GeneID; 70484; -.
DR KEGG; mmu:70484; -.
DR UCSC; uc007qyg.1; mouse. [Q762D5-1]
DR UCSC; uc007qyi.1; mouse. [Q762D5-2]
DR CTD; 11046; -.
DR MGI; MGI:1917734; Slc35d2.
DR eggNOG; KOG1444; Eukaryota.
DR InParanoid; Q762D5; -.
DR OrthoDB; 1093260at2759; -.
DR PhylomeDB; Q762D5; -.
DR TreeFam; TF313307; -.
DR Reactome; R-MMU-2022854; Keratan sulfate biosynthesis.
DR Reactome; R-MMU-2022928; HS-GAG biosynthesis.
DR Reactome; R-MMU-727802; Transport of nucleotide sugars.
DR BioGRID-ORCS; 70484; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Slc35d2; mouse.
DR PRO; PR:Q762D5; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q762D5; protein.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0005461; F:UDP-glucuronic acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005463; F:UDP-N-acetylgalactosamine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005462; F:UDP-N-acetylglucosamine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Golgi apparatus; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..326
FT /note="UDP-N-acetylglucosamine/UDP-glucose/GDP-mannose
FT transporter"
FT /id="PRO_0000313081"
FT TOPO_DOM 1..15
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..130
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..155
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..253
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..280
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 304..326
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT VAR_SEQ 266..283
FT /note="NVSVAYIGMLVGGDYIFS -> HGRWFKIFLFNTQQPVET (in isoform
FT 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_030007"
FT VAR_SEQ 284..326
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_030008"
FT CONFLICT 162
FT /note="V -> I (in Ref. 2; BAE34855)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 326 AA; 35896 MW; F469DC23D635DB2F CRC64;
MEEPNAAPLP SRLARLLSAL FYGTCSFLIV LVNKALLTTY GFPSPIVLGI GQMATTIMIL
YVFKLNKIIH FPDFDKKIPG KLFPLPLLYV GNHISGLSST SKLSLPMFTV LRKFTIPFTL
LLEAIILGTQ YSLNIILSVL AIVLGAFIAA GSDLTFNLEG YVFVFLNDIF TAANGVYTKQ
KMDPKELGKY GVLFYNACFM LIPTVIISVS TGDFQQATEF RHWKNVLFII QFLLSCLLGF
LLMYSTALCS YYNSALTTAV VGAIKNVSVA YIGMLVGGDY IFSLLNFIGL NICMAGGLRY
SFLTLSSQLK PKQPVDEESI PLDLKS
