S35F5_HUMAN
ID S35F5_HUMAN Reviewed; 523 AA.
AC Q8WV83; Q9H6P8; Q9H7D8;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Solute carrier family 35 member F5;
DE AltName: Full=Hepatitis C virus NS5A-transactivated protein 3;
DE Short=HCV NS5A-transactivated protein 3;
GN Name=SLC35F5; Synonyms=NS5ATP3; ORFNames=UNQ2545/PRO6097;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Liu Y., Cheng J., Wang G., Duan H., Zhang L., Chen J., Li L.;
RT "Cloning and identification of human gene 3 transactivated by hepatitis C
RT virus NS5A protein.";
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 154-523 (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=16477629; DOI=10.1002/ijc.21843;
RA Matsuyama R., Togo S., Shimizu D., Momiyama N., Ishikawa T., Ichikawa Y.,
RA Endo I., Kunisaki C., Suzuki H., Hayasizaki Y., Shimada H.;
RT "Predicting 5-fluorouracil chemosensitivity of liver metastases from
RT colorectal cancer using primary tumor specimens: three-gene expression
RT model predicts clinical response.";
RL Int. J. Cancer 119:406-413(2006).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-207, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Putative solute transporter. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8WV83-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8WV83-2; Sequence=VSP_029665, VSP_029666, VSP_029667,
CC VSP_029668;
CC -!- TISSUE SPECIFICITY: Expressed in colorectal cancer cells.
CC {ECO:0000269|PubMed:16477629}.
CC -!- SIMILARITY: Belongs to the SLC35F solute transporter family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB14954.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF529364; AAQ09598.1; -; mRNA.
DR EMBL; AY358620; AAQ88983.1; -; mRNA.
DR EMBL; AK024666; BAB14954.1; ALT_INIT; mRNA.
DR EMBL; AK025657; BAB15206.1; -; mRNA.
DR EMBL; AC017074; AAY14808.1; -; Genomic_DNA.
DR EMBL; CH471103; EAW95176.1; -; Genomic_DNA.
DR EMBL; BC018537; AAH18537.1; -; mRNA.
DR CCDS; CCDS2119.1; -. [Q8WV83-1]
DR RefSeq; NP_001317246.1; NM_001330317.1.
DR RefSeq; NP_079457.2; NM_025181.4. [Q8WV83-1]
DR AlphaFoldDB; Q8WV83; -.
DR BioGRID; 123201; 30.
DR IntAct; Q8WV83; 11.
DR MINT; Q8WV83; -.
DR STRING; 9606.ENSP00000245680; -.
DR TCDB; 2.A.7.24.6; the drug/metabolite transporter (dmt) superfamily.
DR iPTMnet; Q8WV83; -.
DR PhosphoSitePlus; Q8WV83; -.
DR BioMuta; SLC35F5; -.
DR DMDM; 74730818; -.
DR EPD; Q8WV83; -.
DR MassIVE; Q8WV83; -.
DR MaxQB; Q8WV83; -.
DR PaxDb; Q8WV83; -.
DR PeptideAtlas; Q8WV83; -.
DR PRIDE; Q8WV83; -.
DR ProteomicsDB; 74760; -. [Q8WV83-1]
DR ProteomicsDB; 74761; -. [Q8WV83-2]
DR Antibodypedia; 33350; 88 antibodies from 21 providers.
DR DNASU; 80255; -.
DR Ensembl; ENST00000245680.7; ENSP00000245680.2; ENSG00000115084.15. [Q8WV83-1]
DR GeneID; 80255; -.
DR KEGG; hsa:80255; -.
DR MANE-Select; ENST00000245680.7; ENSP00000245680.2; NM_025181.5; NP_079457.2.
DR UCSC; uc002tku.2; human. [Q8WV83-1]
DR CTD; 80255; -.
DR DisGeNET; 80255; -.
DR GeneCards; SLC35F5; -.
DR HGNC; HGNC:23617; SLC35F5.
DR HPA; ENSG00000115084; Low tissue specificity.
DR neXtProt; NX_Q8WV83; -.
DR OpenTargets; ENSG00000115084; -.
DR PharmGKB; PA134953009; -.
DR VEuPathDB; HostDB:ENSG00000115084; -.
DR eggNOG; KOG2765; Eukaryota.
DR GeneTree; ENSGT00390000005949; -.
DR HOGENOM; CLU_026578_2_1_1; -.
DR InParanoid; Q8WV83; -.
DR OMA; KVCRCHK; -.
DR OrthoDB; 1084257at2759; -.
DR PhylomeDB; Q8WV83; -.
DR TreeFam; TF314261; -.
DR PathwayCommons; Q8WV83; -.
DR SignaLink; Q8WV83; -.
DR BioGRID-ORCS; 80255; 8 hits in 1075 CRISPR screens.
DR ChiTaRS; SLC35F5; human.
DR GenomeRNAi; 80255; -.
DR Pharos; Q8WV83; Tdark.
DR PRO; PR:Q8WV83; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8WV83; protein.
DR Bgee; ENSG00000115084; Expressed in adrenal tissue and 183 other tissues.
DR ExpressionAtlas; Q8WV83; baseline and differential.
DR Genevisible; Q8WV83; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..523
FT /note="Solute carrier family 35 member F5"
FT /id="PRO_0000311956"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 420..440
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 452..472
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 252..316
FT /note="EamA"
FT MOD_RES 207
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..6
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_029665"
FT VAR_SEQ 7..13
FT /note="HRGAGRP -> MDSSGWR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_029666"
FT VAR_SEQ 248..250
FT /note="CFV -> ALW (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_029667"
FT VAR_SEQ 251..523
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_029668"
FT CONFLICT 320
FT /note="E -> G (in Ref. 3; BAB14954)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 523 AA; 58887 MW; 1B8CEDF0F1F33179 CRC64;
MVPPRRHRGA GRPGVLSSSP PFRLRSAKFS GIALEDLRRA LKTRLQMVCV FVMNRMNSQN
SGFTQRRRMA LGIVILLLVD VIWVASSELT SYVFTQYNKP FFSTFAKTSM FVLYLLGFII
WKPWRQQCTR GLRGKHAAFF ADAEGYFAAC TTDTTMNSSL SEPLYVPVKF HDLPSEKPES
TNIDTEKTPK KSRVRFSNIM EIRQLPSSHA LEAKLSRMSY PVKEQESILK TVGKLTATQV
AKISFFFCFV WFLANLSYQE ALSDTQVAIV NILSSTSGLF TLILAAVFPS NSGDRFTLSK
LLAVILSIGG VVLVNLAGSE KPAGRDTVGS IWSLAGAMLY AVYIVMIKRK VDREDKLDIP
MFFGFVGLFN LLLLWPGFFL LHYTGFEDFE FPNKVVLMCI IINGLIGTVL SEFLWLWGCF
LTSSLIGTLA LSLTIPLSII ADMCMQKVQF SWLFFAGAIP VFFSFFIVTL LCHYNNWDPV
MVGIRRIFAF ICRKHRIQRV PEDSEQCESL ISMHSVSQED GAS
