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BESA_BACSU
ID   BESA_BACSU              Reviewed;         289 AA.
AC   O32102;
DT   15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Ferri-bacillibactin esterase BesA;
DE            EC=3.1.-.-;
DE   AltName: Full=Bacillibactin trilactone hydrolase;
GN   Name=besA; Synonyms=yuiI; OrderedLocusNames=BSU32010;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [2]
RP   INDUCTION BY FUR.
RC   STRAIN=168;
RX   PubMed=12354229; DOI=10.1046/j.1365-2958.2002.03113.x;
RA   Baichoo N., Wang T., Ye R., Helmann J.D.;
RT   "Global analysis of the Bacillus subtilis Fur regulon and the iron
RT   starvation stimulon.";
RL   Mol. Microbiol. 45:1613-1629(2002).
RN   [3]
RP   FUNCTION AS AN ESTERASE, KINETIC PARAMETERS, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 21332 / IAM 1213;
RX   PubMed=16889643; DOI=10.1111/j.1365-2958.2006.05321.x;
RA   Miethke M., Klotz O., Linne U., May J.J., Beckering C.L., Marahiel M.A.;
RT   "Ferri-bacillibactin uptake and hydrolysis in Bacillus subtilis.";
RL   Mol. Microbiol. 61:1413-1427(2006).
CC   -!- FUNCTION: Catalyzes the hydrolysis of the trilactone cycle of ferri-
CC       bacillibactin (ferri-BB) complex, leading to the formation of
CC       bacillibactin monomers and to cytosolic iron release, thus making iron
CC       available for metabolic use. Can also hydrolyze bacillibactin (BB),
CC       however the catalytic efficiency for ferri-BB hydrolysis is much higher
CC       than for BB. {ECO:0000269|PubMed:16889643}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.54 uM for ferri-bacillibactin complex
CC         {ECO:0000269|PubMed:16889643};
CC         KM=24.4 uM for bacillibactin {ECO:0000269|PubMed:16889643};
CC         KM=221 uM for enterobactin {ECO:0000269|PubMed:16889643};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:16889643}.
CC   -!- INDUCTION: Repressed by fur in the presence of iron.
CC       {ECO:0000269|PubMed:12354229}.
CC   -!- DISRUPTION PHENOTYPE: Impaired growth during iron limitation conditions
CC       and a strong accumulation of intracellular ferri-bacillibactin.
CC       {ECO:0000269|PubMed:16889643}.
CC   -!- SIMILARITY: Belongs to the esterase D family. {ECO:0000305}.
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DR   EMBL; AL009126; CAB15191.2; -; Genomic_DNA.
DR   RefSeq; NP_391081.2; NC_000964.3.
DR   RefSeq; WP_003228740.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O32102; -.
DR   SMR; O32102; -.
DR   STRING; 224308.BSU32010; -.
DR   ESTHER; bacsu-YUII; A85-IroE-IroD-Fes-Yiel.
DR   PaxDb; O32102; -.
DR   PRIDE; O32102; -.
DR   EnsemblBacteria; CAB15191; CAB15191; BSU_32010.
DR   GeneID; 936592; -.
DR   KEGG; bsu:BSU32010; -.
DR   PATRIC; fig|224308.179.peg.3467; -.
DR   eggNOG; COG2819; Bacteria.
DR   InParanoid; O32102; -.
DR   OMA; KPWVEAN; -.
DR   PhylomeDB; O32102; -.
DR   BioCyc; BSUB:BSU32010-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000801; Esterase-like.
DR   Pfam; PF00756; Esterase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Hydrolase; Reference proteome; Serine esterase.
FT   CHAIN           1..289
FT                   /note="Ferri-bacillibactin esterase BesA"
FT                   /id="PRO_0000389644"
FT   ACT_SITE        163
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        225
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        263
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   289 AA;  32439 MW;  44780F01199BC510 CRC64;
     MKEQTTDRTN GGTSNAFTIP GTEVRMMSSR NENRTYHIFI SKPSTPPPPA GYPVIYLLDA
     NSVFGTMTEA VRIQGRRPEK TGVIPAVIVG IGYETAEPFS SARHRDFTMP TAQSKLPERP
     DGREWPEHGG AEGFFRFIEE DLKPEIERDY QIDKKRQTIF GHSLGGLFVL QVLLTKPDAF
     QTYIAGSPSI HWNKPFILKK TDHFVSLTKK NNQPINILLA AGELEQHHKS RMNDNARELY
     ERLAVLSEQG IRAEFCEFSG EGHISVLPVL VSRALRFALH PDGPHLSMG
 
 
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