S35F6_HUMAN
ID S35F6_HUMAN Reviewed; 371 AA.
AC Q8N357; D6W543; Q53GK2; Q8NBX6; Q9NWX0;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Solute carrier family 35 member F6;
DE AltName: Full=ANT2-binding protein;
DE Short=ANT2BP;
DE AltName: Full=Transport and Golgi organization 9 homolog;
DE Flags: Precursor;
GN Name=SLC35F6; Synonyms=C2orf18; ORFNames=UNQ3047/PRO9863;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-length
RT human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT libraries.";
RL DNA Res. 12:117-126(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Blood;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA Greff Z., Keri G., Stemmann O., Mann M.;
RT "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT kinome across the cell cycle.";
RL Mol. Cell 31:438-448(2008).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-365, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [11]
RP FUNCTION, INTERACTION WITH SLC25A5, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=19154410; DOI=10.1111/j.1349-7006.2008.01058.x;
RA Kashiwaya K., Hosokawa M., Eguchi H., Ohigashi H., Ishikawa O.,
RA Shinomura Y., Nakamura Y., Nakagawa H.;
RT "Identification of C2orf18, termed ANT2BP (ANT2-binding protein), as one of
RT the key molecules involved in pancreatic carcinogenesis.";
RL Cancer Sci. 100:457-464(2009).
RN [12]
RP REVIEW, AND SUBCELLULAR LOCATION.
RX PubMed=20957757; DOI=10.1002/pmic.201000196;
RA Schroeder B.A., Wrocklage C., Hasilik A., Saftig P.;
RT "The proteome of lysosomes.";
RL Proteomics 10:4053-4076(2010).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Involved in the maintenance of mitochondrial membrane
CC potential in pancreatic ductal adenocarcinoma (PDAC) cells. Promotes
CC pancreatic ductal adenocarcinoma (PDAC) cell growth. May play a role as
CC a nucleotide-sugar transporter. {ECO:0000269|PubMed:19154410}.
CC -!- SUBUNIT: Interacts with SLC25A5. {ECO:0000269|PubMed:19154410}.
CC -!- INTERACTION:
CC Q8N357; Q13520: AQP6; NbExp=3; IntAct=EBI-713484, EBI-13059134;
CC Q8N357; Q8N350-4: CBARP; NbExp=3; IntAct=EBI-713484, EBI-19051169;
CC Q8N357; Q9HA82: CERS4; NbExp=3; IntAct=EBI-713484, EBI-2622997;
CC Q8N357; P15328: FOLR1; NbExp=3; IntAct=EBI-713484, EBI-2556557;
CC Q8N357; P48165: GJA8; NbExp=3; IntAct=EBI-713484, EBI-17458373;
CC Q8N357; Q8TDT2: GPR152; NbExp=3; IntAct=EBI-713484, EBI-13345167;
CC Q8N357; P05141: SLC25A5; NbExp=2; IntAct=EBI-713484, EBI-355133;
CC Q8N357; Q9NPL8: TIMMDC1; NbExp=3; IntAct=EBI-713484, EBI-6268651;
CC Q8N357; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-713484, EBI-8638294;
CC -!- SUBCELLULAR LOCATION: Mitochondrion. Lysosome membrane; Multi-pass
CC membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed in pancreatic ductal adenocarcinoma
CC (PDAC) (at protein level). Strongly expressed in prostate and thyroid.
CC Weakly expressed in lung, heart, liver and kidney.
CC {ECO:0000269|PubMed:19154410}.
CC -!- SIMILARITY: Belongs to the SLC35F solute transporter family.
CC {ECO:0000305}.
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DR EMBL; AY358820; AAQ89179.1; -; mRNA.
DR EMBL; AK000562; BAA91255.1; -; mRNA.
DR EMBL; AK075164; BAC11445.1; -; mRNA.
DR EMBL; CR457243; CAG33524.1; -; mRNA.
DR EMBL; AK222929; BAD96649.1; -; mRNA.
DR EMBL; AC011740; AAX93265.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00673.1; -; Genomic_DNA.
DR EMBL; CH471053; EAX00676.1; -; Genomic_DNA.
DR EMBL; BC028081; AAH28081.1; -; mRNA.
DR CCDS; CCDS1728.1; -.
DR RefSeq; NP_060347.2; NM_017877.3.
DR AlphaFoldDB; Q8N357; -.
DR SMR; Q8N357; -.
DR BioGRID; 120314; 96.
DR IntAct; Q8N357; 45.
DR MINT; Q8N357; -.
DR STRING; 9606.ENSP00000345528; -.
DR TCDB; 2.A.7.12.13; the drug/metabolite transporter (dmt) superfamily.
DR GlyGen; Q8N357; 2 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q8N357; -.
DR PhosphoSitePlus; Q8N357; -.
DR SwissPalm; Q8N357; -.
DR BioMuta; SLC35F6; -.
DR DMDM; 74728772; -.
DR EPD; Q8N357; -.
DR jPOST; Q8N357; -.
DR MassIVE; Q8N357; -.
DR MaxQB; Q8N357; -.
DR PaxDb; Q8N357; -.
DR PeptideAtlas; Q8N357; -.
DR PRIDE; Q8N357; -.
DR ProteomicsDB; 71766; -.
DR Antibodypedia; 28001; 135 antibodies from 21 providers.
DR DNASU; 54978; -.
DR Ensembl; ENST00000344420.10; ENSP00000345528.5; ENSG00000213699.9.
DR GeneID; 54978; -.
DR KEGG; hsa:54978; -.
DR MANE-Select; ENST00000344420.10; ENSP00000345528.5; NM_017877.4; NP_060347.2.
DR UCSC; uc002rhp.2; human.
DR CTD; 54978; -.
DR DisGeNET; 54978; -.
DR GeneCards; SLC35F6; -.
DR HGNC; HGNC:26055; SLC35F6.
DR HPA; ENSG00000213699; Low tissue specificity.
DR MIM; 619667; gene.
DR neXtProt; NX_Q8N357; -.
DR OpenTargets; ENSG00000213699; -.
DR PharmGKB; PA134913368; -.
DR VEuPathDB; HostDB:ENSG00000213699; -.
DR eggNOG; KOG3912; Eukaryota.
DR GeneTree; ENSGT00390000017237; -.
DR HOGENOM; CLU_025028_1_0_1; -.
DR InParanoid; Q8N357; -.
DR OMA; LWSKWQD; -.
DR OrthoDB; 1169953at2759; -.
DR PhylomeDB; Q8N357; -.
DR TreeFam; TF105890; -.
DR PathwayCommons; Q8N357; -.
DR SignaLink; Q8N357; -.
DR BioGRID-ORCS; 54978; 9 hits in 1084 CRISPR screens.
DR ChiTaRS; SLC35F6; human.
DR GeneWiki; C2orf18; -.
DR GenomeRNAi; 54978; -.
DR Pharos; Q8N357; Tbio.
DR PRO; PR:Q8N357; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8N357; protein.
DR Bgee; ENSG00000213699; Expressed in nipple and 180 other tissues.
DR ExpressionAtlas; Q8N357; baseline and differential.
DR Genevisible; Q8N357; HS.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0005765; C:lysosomal membrane; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:1901029; P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway; IMP:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:UniProtKB.
DR InterPro; IPR009262; SLC35_F1/F2/F6.
DR InterPro; IPR012404; UCP036436.
DR Pfam; PF06027; SLC35F; 1.
DR PIRSF; PIRSF036436; UCP036436; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Lysosome; Membrane; Mitochondrion; Phosphoprotein;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..371
FT /note="Solute carrier family 35 member F6"
FT /id="PRO_0000232514"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 104..160
FT /note="EamA"
FT REGION 352..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 365
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 107
FT /note="V -> A (in Ref. 5; BAD96649)"
FT /evidence="ECO:0000305"
FT CONFLICT 163
FT /note="L -> P (in Ref. 5; BAD96649)"
FT /evidence="ECO:0000305"
FT CONFLICT 215
FT /note="E -> K (in Ref. 2; BAA91255)"
FT /evidence="ECO:0000305"
FT CONFLICT 274
FT /note="A -> V (in Ref. 5; BAD96649)"
FT /evidence="ECO:0000305"
FT CONFLICT 295
FT /note="L -> S (in Ref. 3; BAC11445)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 371 AA; 40214 MW; 225AFE41FA555B12 CRC64;
MAWTKYQLFL AGLMLVTGSI NTLSAKWADN FMAEGCGGSK EHSFQHPFLQ AVGMFLGEFS
CLAAFYLLRC RAAGQSDSSV DPQQPFNPLL FLPPALCDMT GTSLMYVALN MTSASSFQML
RGAVIIFTGL FSVAFLGRRL VLSQWLGILA TIAGLVVVGL ADLLSKHDSQ HKLSEVITGD
LLIIMAQIIV AIQMVLEEKF VYKHNVHPLR AVGTEGLFGF VILSLLLVPM YYIPAGSFSG
NPRGTLEDAL DAFCQVGQQP LIAVALLGNI SSIAFFNFAG ISVTKELSAT TRMVLDSLRT
VVIWALSLAL GWEAFHALQI LGFLILLIGT ALYNGLHRPL LGRLSRGRPL AEESEQERLL
GGTRTPINDA S
