S35G1_HUMAN
ID S35G1_HUMAN Reviewed; 365 AA.
AC Q2M3R5; Q86YG5; Q8NBA5;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2006, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Solute carrier family 35 member G1;
DE AltName: Full=Partner of STIM1;
DE AltName: Full=Transmembrane protein 20;
GN Name=SLC35G1; Synonyms=C10orf60, POST, TMEM20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164054; DOI=10.1038/nature02462;
RA Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L.,
RA Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K.,
RA Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L.,
RA Taylor A., Battles J., Bird C.P., Ainscough R., Almeida J.P.,
RA Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N.,
RA Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A.,
RA Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C.,
RA Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D.,
RA Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C.,
RA Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K.,
RA Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A.,
RA Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S.,
RA McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S.,
RA Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V.,
RA Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A.,
RA Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M.,
RA Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A.,
RA Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P.,
RA Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y.,
RA Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D.,
RA Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 10.";
RL Nature 429:375-381(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, INTERACTION WITH ATP1A1; ATP2A2; ATP2B1; ATP2B4; KPNB1; ORAI1;
RP STIM1 AND XPO1, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=22084111; DOI=10.1073/pnas.1117231108;
RA Krapivinsky G., Krapivinsky L., Stotz S.C., Manasian Y., Clapham D.E.;
RT "POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to
RT multiple transporters.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:19234-19239(2011).
CC -!- FUNCTION: May play a role in intracellular calcium sensing and
CC homeostasis. May act as a negative regulator of plasma membrane
CC calcium-transporting ATPases preventing calcium efflux from the cell.
CC {ECO:0000269|PubMed:22084111}.
CC -!- SUBUNIT: Interacts with STIM1; stimulated by depletion of intracellular
CC calcium. Interacts with ORAI1. Interacts with the plasma membrane
CC calcium-transporting ATPases ATP2B1 and ATP2B4. Interacts with ATP1A1,
CC ATP2A2, KPNB1 and XPO1. {ECO:0000269|PubMed:22084111}.
CC -!- INTERACTION:
CC Q2M3R5; Q15125: EBP; NbExp=3; IntAct=EBI-13311257, EBI-3915253;
CC Q2M3R5; Q96KR6: FAM210B; NbExp=3; IntAct=EBI-13311257, EBI-18938272;
CC Q2M3R5; P54219-3: SLC18A1; NbExp=3; IntAct=EBI-13311257, EBI-17595455;
CC Q2M3R5; Q9NUH8: TMEM14B; NbExp=3; IntAct=EBI-13311257, EBI-8638294;
CC Q2M3R5; Q96Q45-2: TMEM237; NbExp=3; IntAct=EBI-13311257, EBI-10982110;
CC Q2M3R5; Q9Y548: YIPF1; NbExp=3; IntAct=EBI-13311257, EBI-7850136;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22084111};
CC Multi-pass membrane protein {ECO:0000269|PubMed:22084111}. Endoplasmic
CC reticulum membrane {ECO:0000269|PubMed:22084111}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:22084111}. Note=Translocates from the
CC endoplasmic reticulum to the cell membrane in response to a depletion
CC of intracellular calcium and is detected at punctae corresponding to
CC junctions between the endoplasmic reticulum and the cell membrane.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q2M3R5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q2M3R5-2; Sequence=VSP_019566;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:22084111}.
CC -!- SIMILARITY: Belongs to the TMEM20 family. {ECO:0000305}.
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DR EMBL; AK091309; BAC03633.1; -; mRNA.
DR EMBL; AL138923; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC041432; AAH41432.1; -; mRNA.
DR EMBL; BC104814; AAI04815.1; -; mRNA.
DR CCDS; CCDS44459.1; -. [Q2M3R5-1]
DR CCDS; CCDS7432.1; -. [Q2M3R5-2]
DR RefSeq; NP_001128130.1; NM_001134658.2. [Q2M3R5-1]
DR RefSeq; NP_694958.1; NM_153226.3. [Q2M3R5-2]
DR AlphaFoldDB; Q2M3R5; -.
DR SMR; Q2M3R5; -.
DR BioGRID; 127741; 36.
DR IntAct; Q2M3R5; 8.
DR STRING; 9606.ENSP00000400932; -.
DR TCDB; 2.A.7.28.8; the drug/metabolite transporter (dmt) superfamily.
DR iPTMnet; Q2M3R5; -.
DR PhosphoSitePlus; Q2M3R5; -.
DR BioMuta; SLC35G1; -.
DR DMDM; 109895215; -.
DR MassIVE; Q2M3R5; -.
DR MaxQB; Q2M3R5; -.
DR PaxDb; Q2M3R5; -.
DR PeptideAtlas; Q2M3R5; -.
DR PRIDE; Q2M3R5; -.
DR DNASU; 159371; -.
DR Ensembl; ENST00000371408.7; ENSP00000360462.3; ENSG00000176273.15. [Q2M3R5-2]
DR Ensembl; ENST00000427197.2; ENSP00000400932.1; ENSG00000176273.15. [Q2M3R5-1]
DR GeneID; 159371; -.
DR KEGG; hsa:159371; -.
DR MANE-Select; ENST00000427197.2; ENSP00000400932.1; NM_001134658.3; NP_001128130.1.
DR UCSC; uc001kjf.3; human. [Q2M3R5-1]
DR CTD; 159371; -.
DR DisGeNET; 159371; -.
DR GeneCards; SLC35G1; -.
DR HGNC; HGNC:26607; SLC35G1.
DR HPA; ENSG00000176273; Tissue enriched (intestine).
DR MIM; 617167; gene.
DR neXtProt; NX_Q2M3R5; -.
DR OpenTargets; ENSG00000176273; -.
DR PharmGKB; PA134933626; -.
DR VEuPathDB; HostDB:ENSG00000176273; -.
DR eggNOG; KOG4510; Eukaryota.
DR GeneTree; ENSGT00940000153249; -.
DR HOGENOM; CLU_032828_1_2_1; -.
DR InParanoid; Q2M3R5; -.
DR OMA; MGVCVKL; -.
DR OrthoDB; 630849at2759; -.
DR PhylomeDB; Q2M3R5; -.
DR TreeFam; TF323956; -.
DR PathwayCommons; Q2M3R5; -.
DR SignaLink; Q2M3R5; -.
DR BioGRID-ORCS; 159371; 11 hits in 1079 CRISPR screens.
DR GenomeRNAi; 159371; -.
DR Pharos; Q2M3R5; Tbio.
DR PRO; PR:Q2M3R5; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q2M3R5; protein.
DR Bgee; ENSG00000176273; Expressed in secondary oocyte and 123 other tissues.
DR ExpressionAtlas; Q2M3R5; baseline and differential.
DR Genevisible; Q2M3R5; HS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:1990034; P:calcium ion export across plasma membrane; IMP:UniProtKB.
DR GO; GO:0051480; P:regulation of cytosolic calcium ion concentration; IMP:UniProtKB.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Endoplasmic reticulum; Membrane;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..365
FT /note="Solute carrier family 35 member G1"
FT /id="PRO_0000244463"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 80..202
FT /note="EamA 1"
FT DOMAIN 233..357
FT /note="EamA 2"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 60
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_019566"
SQ SEQUENCE 365 AA; 39836 MW; B2B19AEFF5DFFB93 CRC64;
MRPQDSTGVA ELQEPGLPLT DDAPPGATEE PAAAEAAGAP DRGRCWLCLS SPCCSRTEPE
AKKKAPCPGL GLFYTLLSAF LFSVGSLFVK KVQDVHAVEI SAFRCVFQML VVIPCLIYRK
TGFIGPKGQR IFLILRGVLG STAMMLIYYA YQTMSLADAT VITFSSPVFT SIFAWICLKE
KYSPWDALFT VFTITGVILI VRPPFLFGSD TSGMEESYSG HLKGTFAAIG SAVFAASTLV
ILRKMGKSVD YFLSIWYYVV LGLVESVIIL SVLGEWSLPY CGLDRLFLIF IGLFGLGGQI
FITKALQIEK AGPVAIMKTM DVVFAFIFQI IFFNNVPTWW TVGGALCVVA SNVGAAIRKW
YQSSK
