S36A3_HUMAN
ID S36A3_HUMAN Reviewed; 470 AA.
AC Q495N2; Q495N3; Q6ZMU7; Q6ZRU4; Q7Z6B4;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Proton-coupled amino acid transporter 3;
DE Short=Proton/amino acid transporter 3;
DE AltName: Full=Solute carrier family 36 member 3;
DE AltName: Full=Tramdorin-2;
GN Name=SLC36A3; Synonyms=PAT3, TRAMD2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=12809675; DOI=10.1016/s0888-7543(03)00099-5;
RA Boll M., Foltz M., Rubio-Aliaga I., Daniel H.;
RT "A cluster of proton/amino acid transporter genes in the human and mouse
RT genomes.";
RL Genomics 82:47-56(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP GLU-167.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT GLU-167.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=15058382; DOI=10.1007/s00335-003-2319-3;
RA Bermingham J.R. Jr., Pennington J.;
RT "Organization and expression of the SLC36 cluster of amino acid transporter
RT genes.";
RL Mamm. Genome 15:114-125(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q495N2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q495N2-2; Sequence=VSP_032601, VSP_032602;
CC Name=3;
CC IsoId=Q495N2-3; Sequence=VSP_032602;
CC -!- TISSUE SPECIFICITY: Specifically expressed in testis.
CC {ECO:0000269|PubMed:15058382}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC {ECO:0000305}.
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DR EMBL; AY162215; AAO11789.1; -; mRNA.
DR EMBL; AK127978; BAC87215.1; -; mRNA.
DR EMBL; AK131483; BAD18628.1; -; mRNA.
DR EMBL; CH471062; EAW61679.1; -; Genomic_DNA.
DR EMBL; BC101092; AAI01093.1; -; mRNA.
DR EMBL; BC101093; AAI01094.1; -; mRNA.
DR EMBL; BC101094; AAI01095.1; -; mRNA.
DR EMBL; BC101095; AAI01096.1; -; mRNA.
DR CCDS; CCDS4314.1; -. [Q495N2-1]
DR CCDS; CCDS47316.1; -. [Q495N2-3]
DR RefSeq; NP_001138489.1; NM_001145017.1. [Q495N2-3]
DR RefSeq; NP_861439.3; NM_181774.3. [Q495N2-1]
DR AlphaFoldDB; Q495N2; -.
DR BioGRID; 130167; 33.
DR IntAct; Q495N2; 1.
DR STRING; 9606.ENSP00000366942; -.
DR TCDB; 2.A.18.8.4; the amino acid/auxin permease (aaap) family.
DR iPTMnet; Q495N2; -.
DR PhosphoSitePlus; Q495N2; -.
DR BioMuta; SLC36A3; -.
DR DMDM; 172046109; -.
DR PeptideAtlas; Q495N2; -.
DR PRIDE; Q495N2; -.
DR ProteomicsDB; 61968; -. [Q495N2-2]
DR ProteomicsDB; 61969; -. [Q495N2-3]
DR Antibodypedia; 28190; 50 antibodies from 14 providers.
DR DNASU; 285641; -.
DR Ensembl; ENST00000335230.8; ENSP00000334750.3; ENSG00000186334.10. [Q495N2-1]
DR Ensembl; ENST00000377713.3; ENSP00000366942.3; ENSG00000186334.10. [Q495N2-3]
DR GeneID; 285641; -.
DR KEGG; hsa:285641; -.
DR MANE-Select; ENST00000335230.8; ENSP00000334750.3; NM_181774.4; NP_861439.3.
DR UCSC; uc003ltw.3; human. [Q495N2-1]
DR CTD; 285641; -.
DR GeneCards; SLC36A3; -.
DR HGNC; HGNC:19659; SLC36A3.
DR HPA; ENSG00000186334; Tissue enriched (testis).
DR MIM; 608332; gene.
DR neXtProt; NX_Q495N2; -.
DR PharmGKB; PA134875494; -.
DR VEuPathDB; HostDB:ENSG00000186334; -.
DR eggNOG; KOG1304; Eukaryota.
DR GeneTree; ENSGT00940000162406; -.
DR HOGENOM; CLU_009646_0_2_1; -.
DR InParanoid; Q495N2; -.
DR OMA; HAVWGRY; -.
DR OrthoDB; 464614at2759; -.
DR PhylomeDB; Q495N2; -.
DR TreeFam; TF314873; -.
DR PathwayCommons; Q495N2; -.
DR SignaLink; Q495N2; -.
DR BioGRID-ORCS; 285641; 4 hits in 1058 CRISPR screens.
DR GenomeRNAi; 285641; -.
DR Pharos; Q495N2; Tdark.
DR PRO; PR:Q495N2; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q495N2; protein.
DR Bgee; ENSG00000186334; Expressed in testis and 9 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005280; F:amino acid:proton symporter activity; IBA:GO_Central.
DR GO; GO:0015187; F:glycine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015180; F:L-alanine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015193; F:L-proline transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015816; P:glycine transport; IBA:GO_Central.
DR GO; GO:0015808; P:L-alanine transport; IBA:GO_Central.
DR GO; GO:0035524; P:proline transmembrane transport; IBA:GO_Central.
DR GO; GO:1902600; P:proton transmembrane transport; IBA:GO_Central.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..470
FT /note="Proton-coupled amino acid transporter 3"
FT /id="PRO_0000326204"
FT TOPO_DOM 1..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..71
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..185
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 207..210
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 232..252
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..284
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..337
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 359..367
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 389..392
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..425
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 447..470
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 10..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..73
FT /note="MSLLGRDYNSELNSLDNGPQSPSESSSSITSENVHPAGEAGLSMMQTLIHLL
FT KCNIGTGLLGLPLAIKNAGLL -> MGVIISGSLPPFSSPLQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_032601"
FT VAR_SEQ 135
FT /note="R -> RWNLALSPRLECSGKISAHCNPHLQGSSNSPAQASRVAGIYR (in
FT isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_032602"
FT VARIANT 167
FT /note="K -> E (in dbSNP:rs978012)"
FT /evidence="ECO:0000269|PubMed:14702039, ECO:0000269|Ref.3"
FT /id="VAR_040006"
FT VARIANT 185
FT /note="P -> S (in dbSNP:rs12520516)"
FT /id="VAR_040007"
FT VARIANT 190
FT /note="R -> H (in dbSNP:rs17660042)"
FT /id="VAR_040008"
FT VARIANT 415
FT /note="E -> D (in dbSNP:rs13155282)"
FT /id="VAR_040009"
FT VARIANT 421
FT /note="S -> F (in dbSNP:rs13155520)"
FT /id="VAR_040010"
FT CONFLICT 314
FT /note="Q -> R (in Ref. 4; AAI01095)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 470 AA; 51735 MW; AA192584E6BFE848 CRC64;
MSLLGRDYNS ELNSLDNGPQ SPSESSSSIT SENVHPAGEA GLSMMQTLIH LLKCNIGTGL
LGLPLAIKNA GLLVGPVSLL AIGVLTVHCM VILLNCAQHL SQRLQKTFVN YGEATMYGLE
TCPNTWLRAH AVWGRYTVSF LLVITQLGFC SVYFMFMADN LQQMVEKAHV TSNICQPREI
LTLTPILDIR FYMLIILPFL ILLVFIQNLK VLSVFSTLAN ITTLGSMALI FEYIMEGIPY
PSNLPLMANW KTFLLFFGTA IFTFEGVGMV LPLKNQMKHP QQFSFVLYLG MSIVIILYIL
LGTLGYMKFG SDTQASITLN LPNCWLYQSV KLMYSIGIFF TYALQFHVPA EIIIPFAISQ
VSESWALFVD LSVRSALVCL TCVSAILIPR LDLVISLVGS VSSSALALII PALLEIVIFY
SEDMSCVTIA KDIMISIVGL LGCIFGTYQA LYELPQPISH SMANSTGVHA
