S36A4_MOUSE
ID S36A4_MOUSE Reviewed; 500 AA.
AC Q8CH36; Q8C077;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Proton-coupled amino acid transporter 4;
DE Short=Proton/amino acid transporter 4;
DE AltName: Full=Solute carrier family 36 member 4;
GN Name=Slc36a4; Synonyms=Pat4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RA Boll M., Foltz M., Rubio-Aliaga I., Kottra G., Daniel H.;
RT "Identification and characterization a mammalian proton dependent small
RT amino acid cotransporter family.";
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Functions as a sodium-independent electroneutral transporter
CC for tryptophan, proline and alanine. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC {ECO:0000305}.
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DR EMBL; AF453746; AAN76274.1; -; mRNA.
DR EMBL; AK032074; BAC27683.1; -; mRNA.
DR EMBL; BC115964; AAI15965.1; -; mRNA.
DR CCDS; CCDS22840.1; -.
DR RefSeq; NP_758493.2; NM_172289.4.
DR AlphaFoldDB; Q8CH36; -.
DR IntAct; Q8CH36; 1.
DR MINT; Q8CH36; -.
DR STRING; 10090.ENSMUSP00000057355; -.
DR GlyGen; Q8CH36; 1 site.
DR iPTMnet; Q8CH36; -.
DR PhosphoSitePlus; Q8CH36; -.
DR SwissPalm; Q8CH36; -.
DR MaxQB; Q8CH36; -.
DR PaxDb; Q8CH36; -.
DR PRIDE; Q8CH36; -.
DR ProteomicsDB; 256567; -.
DR DNASU; 234967; -.
DR GeneID; 234967; -.
DR KEGG; mmu:234967; -.
DR UCSC; uc009ogg.1; mouse.
DR CTD; 120103; -.
DR MGI; MGI:2442595; Slc36a4.
DR eggNOG; KOG1304; Eukaryota.
DR InParanoid; Q8CH36; -.
DR OrthoDB; 697331at2759; -.
DR PhylomeDB; Q8CH36; -.
DR TreeFam; TF314873; -.
DR Reactome; R-MMU-352230; Amino acid transport across the plasma membrane.
DR Reactome; R-MMU-71240; Tryptophan catabolism.
DR BioGRID-ORCS; 234967; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Slc36a4; mouse.
DR PRO; PR:Q8CH36; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8CH36; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015180; F:L-alanine transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015193; F:L-proline transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015196; F:L-tryptophan transmembrane transporter activity; ISO:MGI.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015808; P:L-alanine transport; ISS:UniProtKB.
DR GO; GO:0015824; P:proline transport; ISO:MGI.
DR GO; GO:0015827; P:tryptophan transport; ISS:UniProtKB.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Amino-acid transport; Glycoprotein; Membrane;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..500
FT /note="Proton-coupled amino acid transporter 4"
FT /id="PRO_0000308319"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q6YBV0"
FT CARBOHYD 492
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 150
FT /note="Q -> S (in Ref. 2; BAC27683 and 3; AAI15965)"
FT /evidence="ECO:0000305"
FT CONFLICT 400
FT /note="R -> C (in Ref. 2; BAC27683 and 3; AAI15965)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 55448 MW; DDD849B6FAAE7E0B CRC64;
MEAPAPAETA GCEELDMDVM RPLINEQNFD GSSDEEQEQT LVPIQKHYQL DGQHGISFLQ
TLVHLLKGNI GTGLLGLPLA IKNAGIVLGP ISLVFIGIIS VHCMHILVRC SHFLCQRFKK
STLGYSDTVS FAMEASPWSC LQRQAAWGRQ VVDFFLVITQ LGFCSVYIVF LAENVKQVHE
GFLGSTPIVS NGSDLSHACE RRSVDLRVYM LCFLPLIILL VFIRELKNLF VLSFLANISM
AASLVIIYQY VVRNMPDPHN LPIVAGWKKY PLFFGTAVFA FEGIGVVLPL ENQMRESKRF
PQALNIGMAI VTVLYISLAT LGYMCFRDEI KGSITLNLPQ DMWLYQSVKI LYSFGIFVTY
SIQFYVPAEI IIPGVTARLH AKWKRICEFG IRSLLVSITR AGAILIPRLD IVISFVGAVS
SSTLALILPP LVEILTFSKD HYNIWMILKN ISIAFTGVVG FLLGTYVTVE EIIYPTTAVV
AGTSQSPFLN VNSTCITSGL
