ID   BESF_STREN              Reviewed;         297 AA.
AC   F8JJ22; G8XHD9;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Probable terminal-alkyne amino-acid exporter {ECO:0000305|PubMed:30867596};
GN   Name=besF {ECO:0000303|PubMed:30867596};
GN   OrderedLocusNames=SCATT_p06920 {ECO:0000312|EMBL:AEW98885.1};
OS   Streptomyces cattleya (strain ATCC 35852 / DSM 46488 / JCM 4925 / NBRC
OS   14057 / NRRL 8057).
OG   Plasmid pSCATT.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1003195;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057;
RA   Ou H.-Y., Li P., Zhao C., O'Hagan D., Deng Z.;
RT   "Complete genome sequence of Streptomyces cattleya strain DSM 46488.";
RL   Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROBABLE FUNCTION.
RC   STRAIN=ATCC 35852 / DSM 46488 / JCM 4925 / NBRC 14057 / NRRL 8057;
RX   PubMed=30867596; DOI=10.1038/s41586-019-1020-y;
RA   Marchand J.A., Neugebauer M.E., Ing M.C., Lin C.I., Pelton J.G.,
RA   Chang M.C.Y.;
RT   "Discovery of a pathway for terminal-alkyne amino acid biosynthesis.";
RL   Nature 567:420-424(2019).
CC   -!- FUNCTION: Probably involved in the export of terminal alkyne-containing
CC       amino acids, namely L-propargylglycine (Pra) and L-beta-ethynylserine,
CC       that are antibiotics synthesized by enzymes encoded in the same gene
CC       cluster. {ECO:0000305|PubMed:30867596}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
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DR   EMBL; CP003229; AEW98885.1; -; Genomic_DNA.
DR   RefSeq; WP_014151493.1; NC_017585.1.
DR   AlphaFoldDB; F8JJ22; -.
DR   SMR; F8JJ22; -.
DR   EnsemblBacteria; AEW98885; AEW98885; SCATT_p06920.
DR   KEGG; scy:SCATT_p06920; -.
DR   PATRIC; fig|1003195.11.peg.1002; -.
DR   HOGENOM; CLU_033863_4_1_11; -.
DR   OrthoDB; 1515911at2; -.
DR   Proteomes; UP000007842; Plasmid pSCATT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   InterPro; IPR000620; EamA_dom.
DR   Pfam; PF00892; EamA; 2.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Membrane; Plasmid; Reference proteome;
KW   Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..297
FT                   /note="Probable terminal-alkyne amino-acid exporter"
FT                   /id="PRO_5003378748"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        212..232
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        249..269
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          6..137
FT                   /note="EamA 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          150..281
FT                   /note="EamA 2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   297 AA;  30538 MW;  065EBB7F28EC7EBC CRC64;
     MKLSKAVWAL VLTVVTWASA FPAIRVGLDG YGVAGLSLSR LTVASVALAI AAGPLGVRRP
     RARDLPMIAL CGATGMSAYQ VLLNWGEVHV PAGTASLLIA IAPVFSVLLA AVFLGERMTW
     AQIAGSAVAI SGAAVIAVAG GHARYTTSAW VVLAAAVVQG VYHFATKPLL ARYTGLEVAC
     YAMWAGTVFL LPLLPAMVHG FATAPVAATA STVYLGLLPS AIGFVSWGYA VARLSVASST
     ASLYLVPPVA LVVAFVWLGE VPPPLALVGG ALGIAGVMLI NRRTLGRRRE ASTPGRR