ID   BEST1_MACFA             Reviewed;         585 AA.
AC   Q6UY87; Q4R348;
DT   12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Bestrophin-1;
DE   AltName: Full=Vitelliform macular dystrophy protein 2;
GN   Name=BEST1; Synonyms=VMD2; ORFNames=QtsA-19642;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RA   Okamoto H., Umeda S., Suzuki M.T., Yoshikawa Y., Tanaka Y., Iwata T.;
RT   "Molecular cloning of VMD2 gene from cynomolgus Monkey (Macaca
RT   fascicularis).";
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Forms calcium-sensitive chloride channels. Permeable to
CC       bicarbonate (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Tetramer or pentamers. May interact with PPP2CB and PPP2R1B
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}. Basolateral cell membrane {ECO:0000250}.
CC   -!- PTM: Phosphorylated by PP2A. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the bestrophin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY357936; AAQ56048.1; -; Genomic_DNA.
DR   EMBL; AY357927; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357928; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357929; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357931; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357933; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357935; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357934; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357932; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357930; AAQ56048.1; JOINED; Genomic_DNA.
DR   EMBL; AY357925; AAQ56049.1; -; mRNA.
DR   EMBL; AB179420; BAE02471.1; -; mRNA.
DR   RefSeq; NP_001270510.1; NM_001283581.1.
DR   AlphaFoldDB; Q6UY87; -.
DR   SMR; Q6UY87; -.
DR   STRING; 9541.XP_005577659.1; -.
DR   GeneID; 101925702; -.
DR   CTD; 7439; -.
DR   eggNOG; KOG3547; Eukaryota.
DR   Proteomes; UP000233100; Unplaced.
DR   GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR   GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR   GO; GO:0005254; F:chloride channel activity; IEA:UniProtKB-KW.
DR   GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR   InterPro; IPR033041; Best1.
DR   InterPro; IPR000615; Bestrophin.
DR   InterPro; IPR021134; Bestrophin/UPF0187.
DR   PANTHER; PTHR10736; PTHR10736; 1.
DR   PANTHER; PTHR10736:SF4; PTHR10736:SF4; 1.
DR   Pfam; PF01062; Bestrophin; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Cell membrane; Chloride; Chloride channel; Ion channel;
KW   Ion transport; Membrane; Phosphoprotein; Reference proteome;
KW   Sensory transduction; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..585
FT                   /note="Bestrophin-1"
FT                   /id="PRO_0000143115"
FT   TOPO_DOM        1..25
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        47..70
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        71..91
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        92..178
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        179..199
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        200..228
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   INTRAMEM        229..249
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        250..270
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        271..291
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        292..585
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        369
FT                   /note="N -> Y (in Ref. 2; BAE02471)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   585 AA;  67488 MW;  5301A65E664268F9 CRC64;
     MTITYTSQVA NARLGSFSRL LLCWRGSIYK LLYGEFFIFL LCYYIIRFIY RLALTEEQQL
     MFEKLTLYCD SYIQLIPISF VLGFYVTLVV TRWWNQYENL PWPDRLMSLV SGFVEGKDEQ
     GRLLRRTLIR YANLGNVLIL RSVSTAVYKR FPSAQHLVQA GFMTPAEHKQ LEKLSLPHNM
     FWVPWVWFAN LSMKAWLGGR IRDPILLQSL LNEMNTLRTQ CGHLYAYDWI SIPLVYTQVV
     TVAVYSFFLT CLVGRQFLNP AKAYPGHELD LVVPVFTFLQ FFFYVGWLKV AEQLINPFGE
     DDDDFETNWI VDRNLQVSLL AVDEMHQDLP RMEPDMYWNE PEPHPPYTAA SAQFRRASFM
     GSTFNISLNK EEMEFQPNQE DKEDAHTGII GRFLGLQSHD HHPPGANSRT KLLWPKRESL
     LHEGLPKNHK AVKQNVRGLE DNKAWKLKAV DAFKSAPLYQ RPGYYSAPQT PLSPTPMFFP
     PEPSVLSKLH SVTGIDTKDK SLKTVSSGAK NSSELLSGSD GALMEHPEVS HVRRKTVEFN
     LTDMPEIPEN HLKEPLELST TNIHATLKDH VDPYWALENR DEAHS