S39AE_XENTR
ID S39AE_XENTR Reviewed; 462 AA.
AC A4IGY6;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Metal cation symporter ZIP14 {ECO:0000250|UniProtKB:Q15043};
DE AltName: Full=Solute carrier family 39 member 14 {ECO:0000250|UniProtKB:Q15043};
DE AltName: Full=Zrt- and Irt-like protein 14 {ECO:0000250|UniProtKB:Q15043};
DE Short=ZIP-14 {ECO:0000250|UniProtKB:Q15043};
DE Flags: Precursor;
GN Name=slc39a14 {ECO:0000250|UniProtKB:Q15043};
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Electroneutral transporter of the plasma membrane mediating
CC the cellular uptake of the divalent metal cations zinc, manganese and
CC iron that are important for tissue homeostasis, metabolism, development
CC and immunity (By similarity). Functions as an energy-dependent
CC symporter, transporting through the membranes an electroneutral complex
CC composed of a divalent metal cation and two bicarbonate anions. Beside
CC these endogenous cellular substrates, can also import cadmium a non-
CC essential metal which is cytotoxic and carcinogenic (By similarity).
CC {ECO:0000250|UniProtKB:Q15043, ECO:0000250|UniProtKB:Q75N73}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 hydrogencarbonate(out) + Zn(2+)(out) = 2
CC hydrogencarbonate(in) + Zn(2+)(in); Xref=Rhea:RHEA:62252,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:Q75N73};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62253;
CC Evidence={ECO:0000250|UniProtKB:Q75N73};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 hydrogencarbonate(out) + Mn(2+)(out) = 2
CC hydrogencarbonate(in) + Mn(2+)(in); Xref=Rhea:RHEA:62260,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:29035;
CC Evidence={ECO:0000250|UniProtKB:Q75N73};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62261;
CC Evidence={ECO:0000250|UniProtKB:Q75N73};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Fe(2+)(out) + 2 hydrogencarbonate(out) = Fe(2+)(in) + 2
CC hydrogencarbonate(in); Xref=Rhea:RHEA:62368, ChEBI:CHEBI:17544,
CC ChEBI:CHEBI:29033; Evidence={ECO:0000250|UniProtKB:Q75N73};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62369;
CC Evidence={ECO:0000250|UniProtKB:Q75N73};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Cd(2+)(out) + 2 hydrogencarbonate(out) = Cd(2+)(in) + 2
CC hydrogencarbonate(in); Xref=Rhea:RHEA:62256, ChEBI:CHEBI:17544,
CC ChEBI:CHEBI:48775; Evidence={ECO:0000250|UniProtKB:Q75N73};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62257;
CC Evidence={ECO:0000250|UniProtKB:Q75N73};
CC -!- SUBUNIT: Homotrimer. {ECO:0000250|UniProtKB:Q15043}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q15043};
CC Multi-pass membrane protein {ECO:0000255}. Apical cell membrane
CC {ECO:0000250|UniProtKB:Q15043}; Multi-pass membrane protein
CC {ECO:0000255}. Basolateral cell membrane
CC {ECO:0000250|UniProtKB:Q15043}; Multi-pass membrane protein
CC {ECO:0000255}. Early endosome membrane {ECO:0000250|UniProtKB:Q15043};
CC Multi-pass membrane protein {ECO:0000255}. Late endosome membrane
CC {ECO:0000250|UniProtKB:Q15043}; Multi-pass membrane protein
CC {ECO:0000255}. Lysosome membrane {ECO:0000250|UniProtKB:Q15043}; Multi-
CC pass membrane protein {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family.
CC {ECO:0000305}.
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DR EMBL; BC135298; AAI35299.1; -; mRNA.
DR RefSeq; NP_001090864.1; NM_001097395.1.
DR AlphaFoldDB; A4IGY6; -.
DR SMR; A4IGY6; -.
DR PaxDb; A4IGY6; -.
DR GeneID; 100038282; -.
DR KEGG; xtr:100038282; -.
DR CTD; 23516; -.
DR Xenbase; XB-GENE-982219; slc39a14.
DR eggNOG; KOG2693; Eukaryota.
DR HOGENOM; CLU_015114_13_0_1; -.
DR InParanoid; A4IGY6; -.
DR OrthoDB; 657777at2759; -.
DR Reactome; R-XTR-442380; Zinc influx into cells by the SLC39 gene family.
DR Proteomes; UP000008143; Chromosome 3.
DR Proteomes; UP000790000; Unplaced.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR GO; GO:0031901; C:early endosome membrane; ISS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:UniProtKB.
DR GO; GO:0031902; C:late endosome membrane; ISS:UniProtKB.
DR GO; GO:0005765; C:lysosomal membrane; ISS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0015296; F:anion:cation symporter activity; ISS:UniProtKB.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; ISS:UniProtKB.
DR GO; GO:0071333; P:cellular response to glucose stimulus; ISS:UniProtKB.
DR GO; GO:0032869; P:cellular response to insulin stimulus; ISS:UniProtKB.
DR GO; GO:0006882; P:cellular zinc ion homeostasis; ISS:UniProtKB.
DR GO; GO:0098739; P:import across plasma membrane; ISS:UniProtKB.
DR GO; GO:0098662; P:inorganic cation transmembrane transport; ISS:UniProtKB.
DR GO; GO:0033212; P:iron import into cell; ISS:UniProtKB.
DR GO; GO:0034755; P:iron ion transmembrane transport; ISS:UniProtKB.
DR GO; GO:0055071; P:manganese ion homeostasis; ISS:UniProtKB.
DR GO; GO:0071421; P:manganese ion transmembrane transport; ISS:UniProtKB.
DR GO; GO:0051344; P:negative regulation of cyclic-nucleotide phosphodiesterase activity; ISS:UniProtKB.
DR GO; GO:0045745; P:positive regulation of G protein-coupled receptor signaling pathway; ISS:UniProtKB.
DR GO; GO:0071578; P:zinc ion import across plasma membrane; ISS:UniProtKB.
DR GO; GO:0071577; P:zinc ion transmembrane transport; ISS:UniProtKB.
DR InterPro; IPR003689; ZIP.
DR Pfam; PF02535; Zip; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Endosome; Glycoprotein; Ion transport; Lysosome; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport;
KW Zinc; Zinc transport.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..462
FT /note="Metal cation symporter ZIP14"
FT /id="PRO_0000312197"
FT TOPO_DOM 17..138
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 160..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..201
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..322
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 344..367
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 389..396
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 418..431
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..452
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 453..462
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 235..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 230..237
FT /note="HHHGHXHX-motif"
FT /evidence="ECO:0000250|UniProtKB:Q15043"
FT MOTIF 346..351
FT /note="XEXPHE-motif"
FT /evidence="ECO:0000250|UniProtKB:Q15043"
FT COMPBIAS 235..255
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..279
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 42
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 68
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 83
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 119
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 462 AA; 50190 MW; CE028E55D5702EAD CRC64;
MPLLLLSALL PFSLMAGPTP STGKELSAAS FLQDILQRYG ENESLSMPQL QSLLENLEVG
KGGGNQRNMS QCLSSSTLFA AHNLTSGSVV DAEGFQSFCP TILQQLETRA CQESPAFQNE
TTPGAEGRPS PGEVWGYGFL CVTVISLCSL FGAGVVPFMK KACYKRLLLF CIALAIGTLF
SNALFQLIPE AFGFNPLEDS YVFTSSVIFG GFYLFFFTEK VLKMMLKQKH EHGHSHYSAD
TSKRDAEEGV TEKLQNGDLD HMIPPPHGSE SDLRGDEKAV QQQDLPGQQS SCYWLKGIRY
SDIGTLAWMI TLSDGLHNFI DGLAIGASFT VSVFQGVSTS IAILCEEFPH ELGDFVILLN
AGMSIPQALF FNFLSACCCY LGLAFGILAG SHFSSNWIFA LAGGMFLYIA LSDMFPEMNE
VSKEDEEGGR AFSAFMIQNA GLLTGFAIML LLTTFSGQIQ LG
