S3AD_ECOLX
ID S3AD_ECOLX Reviewed; 263 AA.
AC P0AG05; P04826;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Aminoglycoside (3'') (9) adenylyltransferase;
DE EC=2.7.7.47;
DE AltName: Full=Aminoglycoside 3''-adenylyltransferase {ECO:0000303|PubMed:2986186};
DE Short=AAD(3'') (9) adenylyltransferase {ECO:0000303|PubMed:2986186, ECO:0000303|PubMed:2997737};
DE AltName: Full=Streptomycin 3''-adenylyltransferase;
GN Name=aadA {ECO:0000303|PubMed:2986186, ECO:0000303|PubMed:2997737};
OS Escherichia coli.
OG Plasmid IncFII R538-1, Plasmid IncN R46, Plasmid RGN238, Plasmid pLMO229,
OG and Plasmid IncN pKM101.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TRANSPOSON=Tn21;
RX PubMed=3054482; DOI=10.1007/bf00339581;
RA Sundstroem L., Radstroem P., Swedberg G., Skoeld O.;
RT "Site-specific recombination promotes linkage between trimethoprim- and
RT sulfonamide resistance genes. Sequence characterization of dhfrV and sulI
RT and a recombination active locus of Tn21.";
RL Mol. Gen. Genet. 213:191-201(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN SPECTINOMYCIN AND
RP STREPTOMYCIN RESISTANCE, AND DISRUPTION PHENOTYPE.
RC TRANSPOSON=Tn7;
RX PubMed=2997737; DOI=10.1093/nar/13.19.7095;
RA Fling M.E., Kopf J., Richards C.;
RT "Nucleotide sequence of the transposon Tn7 gene encoding an aminoglycoside-
RT modifying enzyme, 3''(9)-O-nucleotidyltransferase.";
RL Nucleic Acids Res. 13:7095-7106(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN SPECTINOMYCIN AND
RP STREPTOMYCIN RESISTANCE, AND DISRUPTION PHENOTYPE.
RC PLASMID=IncFII R538-1;
RX PubMed=2986186; DOI=10.1016/0147-619x(85)90052-6;
RA Hollingshead S., Vapnek D.;
RT "Nucleotide sequence analysis of a gene encoding a
RT streptomycin/spectinomycin adenylyltransferase.";
RL Plasmid 13:17-30(1985).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
RC PLASMID=IncN pKM101;
RX PubMed=3037494; DOI=10.1093/nar/15.13.5479;
RA Hall R.M.;
RT "pKM101 is an IS46-promoted deletion of R46.";
RL Nucleic Acids Res. 15:5479-5479(1987).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
RC PLASMID=IncN R46;
RX PubMed=2821509; DOI=10.1093/nar/15.18.7491;
RA Hall R.M., Vockler C.;
RT "The region of the IncN plasmid R46 coding for resistance to beta-lactam
RT antibiotics, streptomycin/spectinomycin and sulphonamides is closely
RT related to antibiotic resistance segments found in IncW plasmids and in
RT Tn21-like transposons.";
RL Nucleic Acids Res. 15:7491-7501(1987).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
RC PLASMID=RGN238;
RX PubMed=2823258; DOI=10.1073/pnas.84.21.7378;
RA Ouellette M., Bissonnette L., Roy P.H.;
RT "Precise insertion of antibiotic resistance determinants into Tn21-like
RT transposons: nucleotide sequence of the OXA-1 beta-lactamase gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:7378-7382(1987).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72 AND 188-263.
RC PLASMID=pLMO229;
RX PubMed=2188588; DOI=10.1128/aac.34.4.642;
RA Sundstroem L., Skoeld O.;
RT "The dhfrI trimethoprim resistance gene of Tn7 can be found at specific
RT sites in other genetic surroundings.";
RL Antimicrob. Agents Chemother. 34:642-650(1990).
CC -!- FUNCTION: Mediates bacterial resistance to the antibiotics streptomycin
CC and spectinomycin. {ECO:0000269|PubMed:2986186,
CC ECO:0000269|PubMed:2997737}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + streptomycin = 3''-O-adenylylstreptomycin + diphosphate;
CC Xref=Rhea:RHEA:20245, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58007, ChEBI:CHEBI:58605; EC=2.7.7.47;
CC Evidence={ECO:0000305|PubMed:2986186, ECO:0000305|PubMed:2997737};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate;
CC Xref=Rhea:RHEA:63228, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:146260, ChEBI:CHEBI:146261;
CC Evidence={ECO:0000305|PubMed:2986186, ECO:0000305|PubMed:2997737};
CC -!- DISRUPTION PHENOTYPE: Loss of resistance to streptomycin and
CC spectinomycin. {ECO:0000269|PubMed:2986186,
CC ECO:0000269|PubMed:2997737}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA26199.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X03043; CAA26848.1; -; Genomic_DNA.
DR EMBL; M86913; AAA72106.1; -; Genomic_DNA.
DR EMBL; X02340; CAA26199.1; ALT_INIT; Genomic_DNA.
DR EMBL; Y00358; CAA68436.1; -; Genomic_DNA.
DR EMBL; X12870; CAA31362.1; -; Genomic_DNA.
DR EMBL; J02967; AAA91587.1; -; Genomic_DNA.
DR EMBL; X17478; CAA35513.1; -; Genomic_DNA.
DR EMBL; X17479; CAA35514.1; -; Genomic_DNA.
DR PIR; A24273; XUECSA.
DR PIR; I40986; I40986.
DR PIR; JH0244; JH0244.
DR PIR; S05476; S05476.
DR PIR; S11704; S11704.
DR PIR; S11707; S11707.
DR RefSeq; NP_065311.1; NC_002525.1.
DR RefSeq; NP_863002.1; NC_004998.1.
DR RefSeq; WP_001206316.1; NZ_WVVS01000006.1.
DR RefSeq; YP_006903505.1; NC_019043.1.
DR RefSeq; YP_006952405.1; NC_019062.1.
DR RefSeq; YP_009068492.1; NC_025141.1.
DR AlphaFoldDB; P0AG05; -.
DR SMR; P0AG05; -.
DR GeneID; 31892376; -.
DR KEGG; ag:CAA26199; -.
DR OMA; VRPWRYP; -.
DR OrthoDB; 1607767at2; -.
DR GO; GO:0070566; F:adenylyltransferase activity; IEA:InterPro.
DR GO; GO:0009012; F:aminoglycoside 3''-adenylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 3.30.460.10; -; 1.
DR InterPro; IPR024172; AadA/Aad9.
DR InterPro; IPR025184; DUF4111.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR002934; Polymerase_NTP_transf_dom.
DR Pfam; PF13427; DUF4111; 1.
DR Pfam; PF01909; NTP_transf_2; 1.
DR PIRSF; PIRSF000819; Streptomycin_3-adenylyltransf; 1.
DR SUPFAM; SSF81301; SSF81301; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; ATP-binding; Nucleotide-binding;
KW Nucleotidyltransferase; Plasmid; Transferase; Transposable element.
FT CHAIN 1..263
FT /note="Aminoglycoside (3'') (9) adenylyltransferase"
FT /id="PRO_0000068579"
FT VARIANT 96
FT /note="I -> M (in transposon Tn21)"
FT VARIANT 128
FT /note="D -> E (in transposon Tn21)"
FT VARIANT 201
FT /note="K -> R (in plasmid pLMO229)"
FT VARIANT 236
FT /note="Missing (in transposon Tn7)"
SQ SEQUENCE 263 AA; 29332 MW; D2CEAC8B7D403FD2 CRC64;
MREAVIAEVS TQLSEVVGVI ERHLEPTLLA VHLYGSAVDG GLKPHSDIDL LVTVTVRLDE
TTRRALINDL LETSASPGES EILRAVEVTI VVHDDIIPWR YPAKRELQFG EWQRNDILAG
IFEPATIDID LAILLTKARE HSVALVGPAA EELFDPVPEQ DLFEALNETL TLWNSPPDWA
GDERNVVLTL SRIWYSAVTG KIAPKDVAAD WAMERLPAQY QPVILEARQA YLGQEEDRLA
SRADQLEEFV HYVKGEITKV VGK
