BEST3_MOUSE
ID BEST3_MOUSE Reviewed; 669 AA.
AC Q6H1V1;
DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Bestrophin-3;
DE AltName: Full=Vitelliform macular dystrophy 2-like protein 3;
GN Name=Best3; Synonyms=Vmd2l3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Heart;
RX PubMed=15218265; DOI=10.1159/000078016;
RA Kraemer F., Stoehr H., Weber B.H.F.;
RT "Cloning and characterization of the murine Vmd2 RFP-TM gene family.";
RL Cytogenet. Genome Res. 105:107-114(2004).
CC -!- FUNCTION: Forms calcium-sensitive chloride channels. Permeable to
CC bicarbonate (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the bestrophin family. {ECO:0000305}.
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DR EMBL; AY450426; AAS09921.1; -; mRNA.
DR CCDS; CCDS24188.1; -.
DR RefSeq; NP_001007584.1; NM_001007583.1.
DR AlphaFoldDB; Q6H1V1; -.
DR SMR; Q6H1V1; -.
DR STRING; 10090.ENSMUSP00000020378; -.
DR TCDB; 1.A.46.1.4; the anion channel-forming bestrophin (bestrophin) family.
DR PhosphoSitePlus; Q6H1V1; -.
DR PaxDb; Q6H1V1; -.
DR PRIDE; Q6H1V1; -.
DR ProteomicsDB; 265208; -.
DR Antibodypedia; 29432; 303 antibodies from 26 providers.
DR DNASU; 382427; -.
DR Ensembl; ENSMUST00000020378; ENSMUSP00000020378; ENSMUSG00000020169.
DR GeneID; 382427; -.
DR KEGG; mmu:382427; -.
DR UCSC; uc007hcp.1; mouse.
DR CTD; 144453; -.
DR MGI; MGI:3580298; Best3.
DR VEuPathDB; HostDB:ENSMUSG00000020169; -.
DR eggNOG; KOG3547; Eukaryota.
DR GeneTree; ENSGT00940000157777; -.
DR HOGENOM; CLU_018069_5_1_1; -.
DR InParanoid; Q6H1V1; -.
DR OMA; DWLWNYE; -.
DR OrthoDB; 279144at2759; -.
DR PhylomeDB; Q6H1V1; -.
DR TreeFam; TF315803; -.
DR Reactome; R-MMU-2672351; Stimuli-sensing channels.
DR BioGRID-ORCS; 382427; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Best3; mouse.
DR PRO; PR:Q6H1V1; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q6H1V1; protein.
DR Bgee; ENSMUSG00000020169; Expressed in hindlimb stylopod muscle and 27 other tissues.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005254; F:chloride channel activity; IDA:MGI.
DR GO; GO:0015698; P:inorganic anion transport; IDA:MGI.
DR GO; GO:0043271; P:negative regulation of ion transport; IDA:MGI.
DR InterPro; IPR000615; Bestrophin.
DR InterPro; IPR021134; Bestrophin/UPF0187.
DR PANTHER; PTHR10736; PTHR10736; 1.
DR Pfam; PF01062; Bestrophin; 1.
PE 2: Evidence at transcript level;
KW Calcium; Cell membrane; Chloride; Chloride channel; Ion channel;
KW Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..669
FT /note="Bestrophin-3"
FT /id="PRO_0000143122"
FT TOPO_DOM 1..25
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..228
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT INTRAMEM 229..249
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..270
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..669
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 399..496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 533..560
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 591..627
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 646..669
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 399..416
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 450..494
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 669 AA; 76431 MW; 83E66FD62BA492A9 CRC64;
MTVTYSSKVA NATFFGFHRL LLKWRGSIYK LLYREFIVFA VLYTAISLVY RLLLTGAQKR
YFEKLSIYCD RYAEQIPVTF VLGFYVTLVV NRWWNQFVNL PWPDRLMLLI SSSVHGSDQH
GRLLRRTLMR YVNLTSLLIF RSVSTAVYKR FPTMDHVVEA GFMTADERKL FDHLKSPHLK
YWVPFIWFGN LATKARNEGR IRDSVDLQSL MTEMNRYRSW CSLLFGYDWV GIPLVYTQVV
TLAVYTFFFA CLIGRQFLDP TKGYVGHDLD LYVPIFTLLQ FFFYAGWLKV AEQLINPFGE
DDDDFETNWC IDRNLQVSLL AVDEMHMSLP KMKKDIYWDD SAARPPYTLA AADYCIPSFL
GSTIQMGLSG SNFPAEDWLW NYEKHGNRHS VMRRVKRFLS THEHPGSPRR RRSFGRQASD
SSMFLPPSPA RDLLDVPSRN PHRGSPTRKQ SRSQEGSPKL HSSMGELSTI RETSRTSTLQ
SLSPQSSVRS SPTKMPQVPE VLITAAEAPA FSADSHQHDS TTSILSLEFT GVQPSGTEQQ
VEPSGTPPGD PNPQTTSAST ERDLFKFEED LEDDRFPKRW SLPEFLESRH TSLGNLGPDP
VSPRDALLLP DTETPSETNG IHPGAGSALA PDILYLMESL DKETDILEFN NEHTGESPKG
TPQRPRTWF
