S40A2_ARATH
ID S40A2_ARATH Reviewed; 512 AA.
AC F4KGN5; Q9LZC8;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Solute carrier family 40 member 2;
DE AltName: Full=Ferroportin-2;
DE AltName: Full=Iron-regulated transporter 2;
DE Short=AtIREG2;
GN Name=IREG2; Synonyms=FPN2; OrderedLocusNames=At5g03570; ORFNames=F12E4.370;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16790430; DOI=10.1074/jbc.m601062200;
RA Schaaf G., Honsbein A., Meda A.R., Kirchner S., Wipf D., von Wiren N.;
RT "AtIREG2 encodes a tonoplast transport protein involved in iron-dependent
RT nickel detoxification in Arabidopsis thaliana roots.";
RL J. Biol. Chem. 281:25532-25540(2006).
CC -!- FUNCTION: Vacuolar transporter that is involved in the transport of
CC excess nickel into the vacuole under iron deficiency, increasing
CC cellular tolerance to nickel under iron deficiency stress response.
CC {ECO:0000269|PubMed:16790430}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000305|PubMed:16790430};
CC Multi-pass membrane protein {ECO:0000305|PubMed:16790430}.
CC Note=Tonoplast.
CC -!- INDUCTION: By iron deficiency in roots. {ECO:0000269|PubMed:16790430}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but plants have increased sensitivity to nickel when grown
CC in an iron-deficient environment. {ECO:0000269|PubMed:16790430}.
CC -!- MISCELLANEOUS: Plants over-expressing IREG2 show increased tolerance to
CC elevated concentrations of nickel. {ECO:0000305|PubMed:16790430}.
CC -!- SIMILARITY: Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX832179; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC Sequence=CAB83320.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL162751; CAB83320.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90628.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90629.1; -; Genomic_DNA.
DR EMBL; BX832179; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T48385; T48385.
DR RefSeq; NP_001190217.1; NM_001203288.1.
DR RefSeq; NP_195977.2; NM_120438.4.
DR AlphaFoldDB; F4KGN5; -.
DR SMR; F4KGN5; -.
DR BioGRID; 17063; 1.
DR STRING; 3702.AT5G03570.2; -.
DR PaxDb; F4KGN5; -.
DR PRIDE; F4KGN5; -.
DR EnsemblPlants; AT5G03570.1; AT5G03570.1; AT5G03570.
DR EnsemblPlants; AT5G03570.2; AT5G03570.2; AT5G03570.
DR GeneID; 831787; -.
DR Gramene; AT5G03570.1; AT5G03570.1; AT5G03570.
DR Gramene; AT5G03570.2; AT5G03570.2; AT5G03570.
DR KEGG; ath:AT5G03570; -.
DR Araport; AT5G03570; -.
DR TAIR; locus:2142649; AT5G03570.
DR eggNOG; KOG2601; Eukaryota.
DR HOGENOM; CLU_020370_1_0_1; -.
DR InParanoid; F4KGN5; -.
DR OMA; RFSSTEM; -.
DR PRO; PR:F4KGN5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KGN5; baseline and differential.
DR Genevisible; F4KGN5; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:UniProtKB.
DR GO; GO:0015087; F:cobalt ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0005381; F:iron ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0010106; P:cellular response to iron ion starvation; IMP:UniProtKB.
DR GO; GO:0055068; P:cobalt ion homeostasis; IMP:TAIR.
DR GO; GO:0006824; P:cobalt ion transport; IMP:TAIR.
DR GO; GO:0006826; P:iron ion transport; IMP:TAIR.
DR GO; GO:0035444; P:nickel cation transmembrane transport; IMP:UniProtKB.
DR GO; GO:0015675; P:nickel cation transport; IMP:TAIR.
DR GO; GO:0000041; P:transition metal ion transport; IMP:TAIR.
DR CDD; cd17480; MFS_SLC40A1_like; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR009716; Ferroportin-1.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR11660; PTHR11660; 1.
DR Pfam; PF06963; FPN1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Ion transport; Membrane; Nickel; Nickel transport; Reference proteome;
KW Stress response; Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..512
FT /note="Solute carrier family 40 member 2"
FT /id="PRO_0000415899"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 512 AA; 57094 MW; F07AA5425B4500F7 CRC64;
MEEETETRVF LSNEQHQEEE EEEEEEPSLP RSMVISLYLG YFLARWGART WEFSVALYMI
YLWPNSLFLT AMYGVVESGS ATLFGPIVGQ MIDGMSYVKV LRLWLVTQNL SFIVAGGAVV
ALLVVPDLKS QNFPVFATLV VLTNLSGAIG VLSTLAGTVL IERDWVVVMS EGHSPAVLTR
MNSVIRGIDL SSKLLSPVIT GLIISFVSLR ASAITFAAWA TITVWIEYWL FISVYNGVPA
IVQSDERRSL RSSQSQAEET DSASSFYVPL LHEEESYRNT QSRSRILRIL ERISESSFVS
AWRNYLNQEI VLPGVSLALL FFTVLSFGTL MTATLEWKGI PTYIIGIGRG ISAGVGLAAT
VLYPLMQSRI SPLRTGVWSF WSQWTCLLVC VGSIWVEKEK IASYMLMAGV AASRLGLWMF
DLAVIQQMQD LVPESDRCVV GGVQNSLQSA LDLMANLLGI IVSNPKDFWM LTLISFATVS
LAGILYTIHL YRIRKHLFHL EKIPLLNNFF AS
