S4A4_PIG
ID S4A4_PIG Reviewed; 1079 AA.
AC Q4U116; Q4U115; Q5EC51; Q5EC52; Q864R7;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Electrogenic sodium bicarbonate cotransporter 1;
DE Short=Sodium bicarbonate cotransporter;
DE AltName: Full=Na(+)/HCO3(-) cotransporter;
DE AltName: Full=Solute carrier family 4 member 4;
GN Name=SLC4A4; Synonyms=NBC1, NBCE1;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RA Pierucci-Alves F., O'Leary T.L., Lorenzen M.D., Schultz B.D.;
RT "Na(+)-HCO cotransporters in porcine vas deferens epithelia.";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 894-985, AND TISSUE SPECIFICITY.
RC TISSUE=Vas deferens;
RX PubMed=12225967; DOI=10.1152/ajpcell.00493.2001;
RA Carlin R.W., Quesnell R.R., Zheng L., Mitchell K.E., Schultz B.D.;
RT "Functional and molecular evidence for Na(+)-HCO cotransporter in porcine
RT vas deferens epithelia.";
RL Am. J. Physiol. 283:C1033-C1044(2002).
CC -!- FUNCTION: Electrogenic sodium/bicarbonate cotransporter with a
CC Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate
CC bicarbonate influx/efflux at the basolateral membrane of cells and
CC regulate intracellular pH. {ECO:0000250|UniProtKB:Q9Y6R1}.
CC -!- FUNCTION: [Isoform 2]: May have a higher activity than isoform 1.
CC {ECO:0000250|UniProtKB:Q9Y6R1}.
CC -!- ACTIVITY REGULATION: Inhibited by stilbene derivatives and regulated by
CC cyclic AMP. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts with CA2/carbonic anhydrase 2 and
CC CA4/carbonic anhydrase 4 which may regulate transporter activity.
CC Isoform 1 but not isoform 2 interacts with AHCYL1 (via PEST domain when
CC phosphorylated); the interaction increases SLC4A4 isoform 1 activity.
CC Interacts with AHCYL2. {ECO:0000250|UniProtKB:O88343,
CC ECO:0000250|UniProtKB:Q9GL77, ECO:0000250|UniProtKB:Q9Y6R1}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane
CC {ECO:0000250|UniProtKB:O88343, ECO:0000250|UniProtKB:Q9Y6R1}; Multi-
CC pass membrane protein {ECO:0000250|UniProtKB:Q9Y6R1}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=NBCe1B, pNBC1;
CC IsoId=Q4U116-1; Sequence=Displayed;
CC Name=2; Synonyms=kNBC1, NBCe1A;
CC IsoId=Q4U116-2; Sequence=VSP_016713, VSP_016714;
CC -!- TISSUE SPECIFICITY: Expressed in vas deferens epithelia (at protein
CC level). {ECO:0000269|PubMed:12225967}.
CC -!- PTM: Phosphorylation of Ser-1026 by PKA increases the binding of CA2
CC and changes the Na(+):HCO3(-) stoichiometry of the transporter from 3:1
CC to 2:1. Phosphorylated in presence of STK39 and dephosphorylated in
CC presence of PP1 phosphatase; phosphorylation seems to inhibit SLC4A4
CC activity. {ECO:0000250|UniProtKB:O88343, ECO:0000250|UniProtKB:Q9Y6R1}.
CC -!- PTM: N-glycosylation is not necessary for the transporter basic
CC functions. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the anion exchanger (TC 2.A.31) family.
CC {ECO:0000305}.
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DR EMBL; AY911643; AAX09639.2; -; mRNA.
DR EMBL; AY911644; AAX09640.1; -; mRNA.
DR EMBL; DQ020174; AAY43214.1; -; mRNA.
DR EMBL; DQ020175; AAY43215.1; -; mRNA.
DR EMBL; AY253302; AAP03886.1; -; mRNA.
DR RefSeq; NP_001025704.1; NM_001030533.1. [Q4U116-2]
DR AlphaFoldDB; Q4U116; -.
DR SMR; Q4U116; -.
DR STRING; 9823.ENSSSCP00000009544; -.
DR PaxDb; Q4U116; -.
DR PeptideAtlas; Q4U116; -.
DR PRIDE; Q4U116; -.
DR GeneID; 503546; -.
DR KEGG; ssc:503546; -.
DR CTD; 8671; -.
DR eggNOG; KOG1172; Eukaryota.
DR InParanoid; Q4U116; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR GO; GO:0016323; C:basolateral plasma membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005452; F:inorganic anion exchanger activity; IEA:InterPro.
DR GO; GO:0008510; F:sodium:bicarbonate symporter activity; ISS:UniProtKB.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015701; P:bicarbonate transport; IBA:GO_Central.
DR GO; GO:0050801; P:ion homeostasis; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; ISS:UniProtKB.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 3.40.930.10; -; 1.
DR InterPro; IPR013769; Band3_cytoplasmic_dom.
DR InterPro; IPR011531; HCO3_transpt-like_TM_dom.
DR InterPro; IPR003020; HCO3_transpt_euk.
DR InterPro; IPR003024; Na/HCO3_transpt.
DR InterPro; IPR016152; PTrfase/Anion_transptr.
DR PANTHER; PTHR11453; PTHR11453; 1.
DR Pfam; PF07565; Band_3_cyto; 1.
DR Pfam; PF00955; HCO3_cotransp; 1.
DR PRINTS; PR01231; HCO3TRNSPORT.
DR PRINTS; PR01232; NAHCO3TRSPRT.
DR SUPFAM; SSF55804; SSF55804; 1.
DR TIGRFAMs; TIGR00834; ae; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Disulfide bond; Glycoprotein;
KW Ion transport; Membrane; Phosphoprotein; Reference proteome; Sodium;
KW Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1079
FT /note="Electrogenic sodium bicarbonate cotransporter 1"
FT /id="PRO_0000079229"
FT TOPO_DOM 1..466
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 467..491
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 492..501
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 502..520
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 521
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 522..542
FT /note="Discontinuously helical; Name=3"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 543..550
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 551..571
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 572..585
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 586..609
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 610..692
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 693..710
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 711..725
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 726..745
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 746..779
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 780..807
FT /note="Helical; Name=8"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 808..819
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 820..836
FT /note="Helical; Name=9"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 837
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 838..855
FT /note="Discontinuously helical; Name=10"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 856..877
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 878..894
FT /note="Helical; Name=11"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 895..901
FT /note="Extracellular"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TRANSMEM 902..918
FT /note="Helical; Name=12"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 919..960
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT INTRAMEM 961..986
FT /note="Discontinuously helical"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT TOPO_DOM 987..1079
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT REGION 1..62
FT /note="Required for interaction with AHCYL1"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT REGION 39..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 238..265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 748..779
FT /note="Interaction with CA4"
FT /evidence="ECO:0000250"
FT REGION 1002..1004
FT /note="CA2-binding"
FT /evidence="ECO:0000250"
FT REGION 1012..1079
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1030..1033
FT /note="CA2-binding"
FT /evidence="ECO:0000250"
FT REGION 1057..1059
FT /note="Required for basolateral targeting"
FT /evidence="ECO:0000250"
FT COMPBIAS 39..53
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 54..73
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 238..260
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1012..1039
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1060..1079
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 30
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 65
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 68
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 223
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 232
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 233
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 245
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 249
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9JI66"
FT MOD_RES 254
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT MOD_RES 256
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 257
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT MOD_RES 262
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 1026
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT MOD_RES 1029
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 1034
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT MOD_RES 1044
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O88343"
FT MOD_RES 1069
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9JI66"
FT CARBOHYD 641
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 661
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
FT DISULFID 627..629
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT DISULFID 674..686
FT /evidence="ECO:0000250|UniProtKB:Q9Y6R1"
FT VAR_SEQ 1..44
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_016713"
FT VAR_SEQ 45..85
FT /note="HKRKAGHREKKEKERVSENYSDKSDVENADESSSSILKPLI -> MSTESVE
FT GKSNSSGDRGRARSYSFLRVVQPMFNHSIFTSAV (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_016714"
SQ SEQUENCE 1079 AA; 121410 MW; 8DA4F2AB3734A4F9 CRC64;
MEDEAALDRG ASFLKHVCDE EEVEGHHTIY IGVHVPKSYR RRRRHKRKAG HREKKEKERV
SENYSDKSDV ENADESSSSI LKPLISPAAE RIRFILGEED DSPAPPQLFT ELDELLAVDG
QEMEWKETAR WIKFEEKVEQ GGERWSKPHV ATLSLHSLFE LRTCMEKGSI MLDREAASLP
QLVEMIVDHQ IETGLLKPDL KDKVTYTLLR KHRHQTKKSN LRSLADIGKT VSSASRMFTS
PENGSPAMTH RNLTSSSLND ISDKPEKDQL KNKFMKKLPR DAEASNVLVG EVDFLDSPFI
AFVRLQQAVM LGALTEVPVP TRFLFILLGP KGKAKSYHEI GRAIATLMSD EVFHDIAYKA
KDRQDLIAGI DEFLDEVIVL PPGEWDPAIR IEPPKSLPSS DKRKNMYSGG ENVQMNGDTP
HDGGHGGGGH ADCEELQRTG RFCGGLIKDI KRKAPFFASD FYDALNIQAL SAILFIYLAT
VTNAITFGGL LGDATDNMQG VLESFLGTAV SGAVFCLFAG QPLTILSSTG PVLVFERLLF
NFSKDHNFDY LEFRLWIGLW SAFLCLILVA TDASFLVQYF TRFTEEGFSS LISFIFIYDA
FKKMIKLADY YPINSNFKVG YNTQFSCVCV PPNPVNISVS NDTTLAPEDL QTVSSADMYH
NATFDWALLT KKECLKYEGK LVGNNCDFVP DITLMSFILF LGTYTSSMAL KKFKTSRYFP
TTARKLISDF AIILSILIFC VIDALVGVDT PKLIVPSEFK PTSPNRGWFV PPFGGNPWWV
YLAAAIPALL VTILIFMDQQ ITAVIVNRKE HKLKKGAGYH LDLFWVAILM VVCSFMALPW
YVAATVISIA HIDSLKMETE TSAPGEQPKF LGVREQRVTG TLVFILTGLS VFMAPILKFI
PMPVLYGVFL YMGVASLNGV QFMDRLKLLL MPLKHQPDFI YLRHVPLRRV HLFTFLQVLC
LALLWILKST VAAIIFPVMI LALVAVRKGM DYLFSQHDLS FLDDVIPEKD KKKKEDEKKK
KKKKGSLDSD SDDSDCPYSE KVPSIKIPMD IMEQQPFLSD SKPSDRERSP TFLERHTSC
