S52A2_RAT
ID S52A2_RAT Reviewed; 450 AA.
AC B5MEV3; A3KN99;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Solute carrier family 52, riboflavin transporter, member 2;
DE AltName: Full=Porcine endogenous retrovirus A receptor 2;
DE AltName: Full=Protein GPR172B;
DE AltName: Full=Riboflavin transporter 1;
DE Short=rRFT1;
GN Name=Slc52a2; Synonyms=Gpr172a, Gpr172b, Rft1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Kidney;
RX PubMed=18632736; DOI=10.1152/ajpcell.00019.2008;
RA Yonezawa A., Masuda S., Katsura T., Inui K.;
RT "Identification and functional characterization of a novel human and rat
RT riboflavin transporter, RFT1.";
RL Am. J. Physiol. 295:C632-C641(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=19122205; DOI=10.1093/jb/mvn181;
RA Yamamoto S., Inoue K., Ohta K.Y., Fukatsu R., Maeda J.Y., Yoshida Y.,
RA Yuasa H.;
RT "Identification and functional characterization of rat riboflavin
RT transporter 2.";
RL J. Biochem. 145:437-443(2009).
CC -!- FUNCTION: Plasma membrane transporter mediating the uptake by cells of
CC the water soluble vitamin B2/riboflavin that plays a key role in
CC biochemical oxidation-reduction reactions of the carbohydrate, lipid,
CC and amino acid metabolism. May also act as a receptor for 4-
CC hydroxybutyrate. {ECO:0000250|UniProtKB:Q9HAB3}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=riboflavin(in) = riboflavin(out); Xref=Rhea:RHEA:35015,
CC ChEBI:CHEBI:57986; Evidence={ECO:0000250|UniProtKB:Q9HAB3};
CC -!- ACTIVITY REGULATION: Riboflavin transport is Na(+)-independent but
CC moderately pH-sensitive (By similarity). Activity is strongly inhibited
CC by riboflavin analogs, such as lumiflavin (By similarity). Weakly
CC inhibited by flavin adenine dinucleotide (FAD) and flavin
CC mononucleotide (FMN) (By similarity). {ECO:0000250|UniProtKB:Q9HAB3}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18632736,
CC ECO:0000269|PubMed:19122205}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the placenta and small
CC intestine, moderately in the kidney, colon, lung, prostate, uterus, and
CC thymus, and weakly in all other tissues. {ECO:0000269|PubMed:18632736}.
CC -!- SIMILARITY: Belongs to the riboflavin transporter family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB362535; BAG71130.1; -; mRNA.
DR EMBL; BC133726; AAI33727.1; -; mRNA.
DR EMBL; CH473950; EDM15963.1; -; Genomic_DNA.
DR RefSeq; NP_001103140.1; NM_001109670.1.
DR RefSeq; XP_006241888.1; XM_006241826.3.
DR AlphaFoldDB; B5MEV3; -.
DR STRING; 10116.ENSRNOP00000046721; -.
DR GlyGen; B5MEV3; 1 site.
DR PhosphoSitePlus; B5MEV3; -.
DR PaxDb; B5MEV3; -.
DR Ensembl; ENSRNOT00000047380; ENSRNOP00000046721; ENSRNOG00000032561.
DR GeneID; 362942; -.
DR KEGG; rno:362942; -.
DR UCSC; RGD:1560410; rat.
DR CTD; 79581; -.
DR RGD; 1560410; Slc52a2.
DR eggNOG; KOG4255; Eukaryota.
DR GeneTree; ENSGT00390000003774; -.
DR HOGENOM; CLU_034789_1_0_1; -.
DR InParanoid; B5MEV3; -.
DR OMA; WCGISIQ; -.
DR OrthoDB; 757564at2759; -.
DR PhylomeDB; B5MEV3; -.
DR TreeFam; TF314820; -.
DR Reactome; R-RNO-196843; Vitamin B2 (riboflavin) metabolism.
DR PRO; PR:B5MEV3; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Proteomes; UP000234681; Chromosome 7.
DR Bgee; ENSRNOG00000032561; Expressed in jejunum and 18 other tissues.
DR Genevisible; B5MEV3; RN.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0062124; F:4-hydroxybutyrate receptor activity; ISS:UniProtKB.
DR GO; GO:0032217; F:riboflavin transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0032218; P:riboflavin transport; IDA:UniProtKB.
DR InterPro; IPR009357; Riboflavin_transptr.
DR PANTHER; PTHR12929; PTHR12929; 1.
DR Pfam; PF06237; DUF1011; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..450
FT /note="Solute carrier family 52, riboflavin transporter,
FT member 2"
FT /id="PRO_0000399789"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 230..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 178
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 426
FT /note="I -> V (in Ref. 1; BAG71130)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 450 AA; 46956 MW; 1D4ABA0DC5C5B955 CRC64;
MAAPPLGRLV LTHLLVALFG MGSWIAVNGI WVELPVVVKE LPEGWSLPSY LSVLVALGNL
GLLLVTLWRR LAPGKSERIP IQVVQGLSIV GTGLLAPLWS NMALVAGQLH SVAFLTLAFV
LALSCCASNV TFLPFLSHLP PPFLRSFFLG QGLSALLPCV LALAQGVGRL ECLHVPANGT
TGPPIKVSPI NFPERFSAGT FFWVLTALLG TSAAAFQGLL LLLPSPPPEA TMGTGLRVET
PGTEEEEEEE EASPLQEPPG QVASIVSSPD PKAHRLFSSR SACLLGLLAI TNALTNGVLP
AVQSFSCLPY GRLAYHLAVV LGSSANPLAC FLAMAVLCRS LAGLYGLCLL GMFFGTYLMT
LAVLSPCPPL VGTSAGVVLV VLSWVLCAGV FSYIKVATSS MLHSGGRPAL LAAGVAIQVG
SLLGAIAMFP PTSVYPVFRS GEDCVDQCGP
