S5A1_BOVIN
ID S5A1_BOVIN Reviewed; 257 AA.
AC A5PJS2;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=3-oxo-5-alpha-steroid 4-dehydrogenase 1;
DE EC=1.3.1.22 {ECO:0000250|UniProtKB:P18405};
DE AltName: Full=SR type 1;
DE AltName: Full=Steroid 5-alpha-reductase 1;
DE Short=S5AR 1;
GN Name=SRD5A1;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal skin;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Converts testosterone into 5-alpha-dihydrotestosterone and
CC progesterone or corticosterone into their corresponding 5-alpha-3-
CC oxosteroids. It plays a central role in sexual differentiation and
CC androgen physiology. {ECO:0000250|UniProtKB:P24008}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid +
CC H(+) + NADPH; Xref=Rhea:RHEA:54384, ChEBI:CHEBI:13601,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47909, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:54386;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5alpha-pregnane-3,20-dione + NADP(+) = H(+) + NADPH +
CC progesterone; Xref=Rhea:RHEA:21952, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17026, ChEBI:CHEBI:28952, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:21954;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=17beta-hydroxy-5alpha-androstan-3-one + NADP(+) = H(+) + NADPH
CC + testosterone; Xref=Rhea:RHEA:50820, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16330, ChEBI:CHEBI:17347, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50822;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=androst-4-ene-3,17-dione + H(+) + NADPH = 5alpha-androstan-
CC 3,17-dione + NADP(+); Xref=Rhea:RHEA:50816, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15994, ChEBI:CHEBI:16422, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; Evidence={ECO:0000250|UniProtKB:P24008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50817;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC -!- SUBCELLULAR LOCATION: Microsome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Endoplasmic reticulum membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the steroid 5-alpha reductase family.
CC {ECO:0000305}.
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DR EMBL; BC142219; AAI42220.1; -; mRNA.
DR RefSeq; NP_001092607.1; NM_001099137.1.
DR RefSeq; XP_015314613.1; XM_015459127.1.
DR AlphaFoldDB; A5PJS2; -.
DR SMR; A5PJS2; -.
DR STRING; 9913.ENSBTAP00000020570; -.
DR PaxDb; A5PJS2; -.
DR PRIDE; A5PJS2; -.
DR Ensembl; ENSBTAT00000020570; ENSBTAP00000020570; ENSBTAG00000015478.
DR GeneID; 614612; -.
DR KEGG; bta:614612; -.
DR CTD; 6715; -.
DR VEuPathDB; HostDB:ENSBTAG00000015478; -.
DR VGNC; VGNC:35271; SRD5A1.
DR eggNOG; KOG1638; Eukaryota.
DR GeneTree; ENSGT00950000182886; -.
DR HOGENOM; CLU_065395_1_1_1; -.
DR InParanoid; A5PJS2; -.
DR OMA; WLEWLGF; -.
DR OrthoDB; 1574389at2759; -.
DR TreeFam; TF314668; -.
DR Reactome; R-BTA-193048; Androgen biosynthesis.
DR Proteomes; UP000009136; Chromosome 20.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003865; F:3-oxo-5-alpha-steroid 4-dehydrogenase activity; ISS:UniProtKB.
DR GO; GO:0047751; F:cholestenone 5-alpha-reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0050213; F:progesterone 5-alpha-reductase activity; IEA:RHEA.
DR GO; GO:0006702; P:androgen biosynthetic process; IEA:UniProt.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006694; P:steroid biosynthetic process; IBA:GO_Central.
DR InterPro; IPR016636; 3-oxo-5-alpha-steroid_4-DH.
DR InterPro; IPR001104; 3-oxo-5_a-steroid_4-DH_C.
DR InterPro; IPR039357; SRD5A/TECR.
DR PANTHER; PTHR10556; PTHR10556; 1.
DR Pfam; PF02544; Steroid_dh; 1.
DR PIRSF; PIRSF015596; 5_alpha-SR2; 1.
DR PROSITE; PS50244; S5A_REDUCTASE; 1.
PE 2: Evidence at transcript level;
KW Differentiation; Endoplasmic reticulum; Lipid metabolism; Membrane;
KW Microsome; NADP; Oxidoreductase; Reference proteome;
KW Sexual differentiation; Transmembrane; Transmembrane helix.
FT CHAIN 1..257
FT /note="3-oxo-5-alpha-steroid 4-dehydrogenase 1"
FT /id="PRO_0000317710"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 257 AA; 29186 MW; 2A49937DFB45FFB0 CRC64;
MELAERFLLD ALAYLECALG VVCYVLLKLV GSPYGRYASS GSAFGLPARA AWTVQELPSL
ALPLLACAGA GAPAERLNRW PNCILLAMFL VHYAQRSLVF PFLIRGGKPM PLYAFLLAFI
FCTYNGYLQS RYLSQYAVYA DDWLSDPRFL TGSALWLIGM LINIHSDHVL RNLRKPGETG
YKIPRGGLFE YISAANYFGE VVEWCGYALA SWSIQGWAFA VFTFCVLFTR AQQHHKWYHE
KFEDYPKFRK IMIPFLV
