S5A1_MOUSE
ID S5A1_MOUSE Reviewed; 255 AA.
AC Q68FF9; Q505K7;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 2.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=3-oxo-5-alpha-steroid 4-dehydrogenase 1 {ECO:0000305};
DE EC=1.3.1.22 {ECO:0000250|UniProtKB:P18405};
DE AltName: Full=SR type 1;
DE AltName: Full=Steroid 5-alpha-reductase 1;
DE Short=S5AR 1;
GN Name=Srd5a1 {ECO:0000312|MGI:MGI:98400};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Converts testosterone into 5-alpha-dihydrotestosterone and
CC progesterone or corticosterone into their corresponding 5-alpha-3-
CC oxosteroids. It plays a central role in sexual differentiation and
CC androgen physiology. {ECO:0000250|UniProtKB:P24008}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-5alpha-steroid + NADP(+) = a 3-oxo-Delta(4)-steroid +
CC H(+) + NADPH; Xref=Rhea:RHEA:54384, ChEBI:CHEBI:13601,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47909, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:54386;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5alpha-pregnane-3,20-dione + NADP(+) = H(+) + NADPH +
CC progesterone; Xref=Rhea:RHEA:21952, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17026, ChEBI:CHEBI:28952, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:21954;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=17beta-hydroxy-5alpha-androstan-3-one + NADP(+) = H(+) + NADPH
CC + testosterone; Xref=Rhea:RHEA:50820, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16330, ChEBI:CHEBI:17347, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.3.1.22;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50822;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=androst-4-ene-3,17-dione + H(+) + NADPH = 5alpha-androstan-
CC 3,17-dione + NADP(+); Xref=Rhea:RHEA:50816, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15994, ChEBI:CHEBI:16422, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; Evidence={ECO:0000250|UniProtKB:P24008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50817;
CC Evidence={ECO:0000250|UniProtKB:P24008};
CC -!- SUBCELLULAR LOCATION: Microsome membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Endoplasmic reticulum membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the steroid 5-alpha reductase family.
CC {ECO:0000305}.
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DR EMBL; BC079863; AAH79863.1; -; mRNA.
DR EMBL; BC094503; AAH94503.1; -; mRNA.
DR CCDS; CCDS26624.1; -.
DR RefSeq; NP_780492.2; NM_175283.3.
DR AlphaFoldDB; Q68FF9; -.
DR SMR; Q68FF9; -.
DR STRING; 10090.ENSMUSP00000089097; -.
DR iPTMnet; Q68FF9; -.
DR PhosphoSitePlus; Q68FF9; -.
DR jPOST; Q68FF9; -.
DR MaxQB; Q68FF9; -.
DR PaxDb; Q68FF9; -.
DR PRIDE; Q68FF9; -.
DR ProteomicsDB; 260801; -.
DR DNASU; 78925; -.
DR GeneID; 78925; -.
DR KEGG; mmu:78925; -.
DR UCSC; uc007rck.1; mouse.
DR CTD; 6715; -.
DR MGI; MGI:98400; Srd5a1.
DR eggNOG; KOG1638; Eukaryota.
DR InParanoid; Q68FF9; -.
DR OrthoDB; 1574389at2759; -.
DR PhylomeDB; Q68FF9; -.
DR TreeFam; TF314668; -.
DR Reactome; R-MMU-193048; Androgen biosynthesis.
DR BioGRID-ORCS; 78925; 0 hits in 75 CRISPR screens.
DR ChiTaRS; Srd5a1; mouse.
DR PRO; PR:Q68FF9; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q68FF9; protein.
DR GO; GO:0070852; C:cell body fiber; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0043209; C:myelin sheath; ISO:MGI.
DR GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR GO; GO:0003865; F:3-oxo-5-alpha-steroid 4-dehydrogenase activity; IMP:MGI.
DR GO; GO:0033218; F:amide binding; ISO:MGI.
DR GO; GO:0047751; F:cholestenone 5-alpha-reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0070402; F:NADPH binding; ISO:MGI.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0050213; F:progesterone 5-alpha-reductase activity; IEA:RHEA.
DR GO; GO:0006702; P:androgen biosynthetic process; IMP:MGI.
DR GO; GO:0006710; P:androgen catabolic process; ISO:MGI.
DR GO; GO:0008209; P:androgen metabolic process; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0042448; P:progesterone metabolic process; ISO:MGI.
DR GO; GO:0009410; P:response to xenobiotic stimulus; ISO:MGI.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR GO; GO:0006694; P:steroid biosynthetic process; ISO:MGI.
DR InterPro; IPR016636; 3-oxo-5-alpha-steroid_4-DH.
DR InterPro; IPR001104; 3-oxo-5_a-steroid_4-DH_C.
DR InterPro; IPR039357; SRD5A/TECR.
DR PANTHER; PTHR10556; PTHR10556; 1.
DR Pfam; PF02544; Steroid_dh; 1.
DR PIRSF; PIRSF015596; 5_alpha-SR2; 1.
DR PROSITE; PS50244; S5A_REDUCTASE; 1.
PE 2: Evidence at transcript level;
KW Differentiation; Endoplasmic reticulum; Lipid metabolism; Membrane;
KW Microsome; NADP; Oxidoreductase; Reference proteome;
KW Sexual differentiation; Transmembrane; Transmembrane helix.
FT CHAIN 1..255
FT /note="3-oxo-5-alpha-steroid 4-dehydrogenase 1"
FT /id="PRO_0000317711"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 7
FT /note="C -> R (in Ref. 1; AAH79863)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="V -> I (in Ref. 1; AAH94503)"
FT /evidence="ECO:0000305"
FT CONFLICT 176
FT /note="E -> D (in Ref. 1; AAH94503)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 255 AA; 29344 MW; 80A5624B25D46D1A CRC64;
MELDELCLLD ALVYLEGFLA FVAFVGLQMV GSSYGRYSSQ WSGRRVPARP AWFLQELPSM
AWPLYECIRP AAARLGNLPN RVLLAMFLIH YVQRTLVFPV LIRGGKPTLL FTFVLAFLFC
TLNGYLQSRY LSQFAVYAED WVTHPCFLTG FALWLVGMVI NIHSDHILRN LRKPGETGYK
IPRGGLFEYV SSANYFGELV EWCGFALASW SLQGVVFALF TLCALFTRAR QHHQWYLEKF
EDYPKTRKIL IPFLL
