S5A4A_MOUSE
ID S5A4A_MOUSE Reviewed; 656 AA.
AC Q9ET37;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Solute carrier family 5 member 4A {ECO:0000312|MGI:MGI:1927848};
GN Name=Slc5a4a {ECO:0000312|MGI:MGI:1927848};
GN Synonyms=Sglt3a {ECO:0000303|PubMed:22301059};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=129/Sv;
RX PubMed=11042146; DOI=10.1101/gr.146600;
RA Pletcher M.T., Roe B.A., Chen F., Do T., Do A., Malaj E., Reeves R.H.;
RT "Chromosome evolution: the junction of mammalian chromosomes in the
RT formation of mouse chromosome 10.";
RL Genome Res. 10:1463-1467(2000).
RN [2]
RP FUNCTION, LACK OF SUGAR TRANSPORT ACTIVITY, ACTIVITY REGULATION, TISSUE
RP SPECIFICITY, AND MUTAGENESIS OF GLU-457.
RX PubMed=22301059; DOI=10.1152/ajpcell.00436.2011;
RA Barcelona S., Menegaz D., Diez-Sampedro A.;
RT "Mouse SGLT3a generates proton-activated currents but does not transport
RT sugar.";
RL Am. J. Physiol. 302:C1073-C1082(2012).
CC -!- FUNCTION: Has sugar-induced sodium-independent electrogenic activity.
CC Generation of glucose-induced inward currents is pH-dependent, with
CC activity in acidic conditions (pH 5) but not neutral conditions. Does
CC not have sugar transport activity. {ECO:0000269|PubMed:22301059}.
CC -!- ACTIVITY REGULATION: Not inhibited by phlorizin.
CC {ECO:0000269|PubMed:22301059}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:22301059};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in small intestine.
CC {ECO:0000269|PubMed:22301059}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; AF251267; AAG01741.1; -; mRNA.
DR CCDS; CCDS35941.1; -.
DR AlphaFoldDB; Q9ET37; -.
DR SMR; Q9ET37; -.
DR STRING; 10090.ENSMUSP00000020450; -.
DR GlyGen; Q9ET37; 1 site.
DR iPTMnet; Q9ET37; -.
DR PhosphoSitePlus; Q9ET37; -.
DR PaxDb; Q9ET37; -.
DR PRIDE; Q9ET37; -.
DR ProteomicsDB; 256905; -.
DR MGI; MGI:1927848; Slc5a4a.
DR eggNOG; KOG2349; Eukaryota.
DR InParanoid; Q9ET37; -.
DR PhylomeDB; Q9ET37; -.
DR Reactome; R-MMU-189200; Cellular hexose transport.
DR PRO; PR:Q9ET37; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q9ET37; protein.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005412; F:glucose:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0015078; F:proton transmembrane transporter activity; IDA:MGI.
DR GO; GO:1902600; P:proton transmembrane transport; IDA:MGI.
DR GO; GO:0006814; P:sodium ion transport; IBA:GO_Central.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR InterPro; IPR018212; Na/solute_symporter_CS.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS00457; NA_SOLUT_SYMP_2; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..656
FT /note="Solute carrier family 5 member 4A"
FT /id="PRO_0000105377"
FT TOPO_DOM 1..28
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..64
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..105
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..171
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 192..208
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..270
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..314
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..423
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..455
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..476
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 477..526
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 527..547
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 548..634
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 635..655
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 574..593
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 248
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 457
FT /note="E->Q: Confers sodium-dependent sugar transport
FT activity not found in the wild type protein."
FT /evidence="ECO:0000269|PubMed:22301059"
SQ SEQUENCE 656 AA; 71838 MW; A6668E815204D39B CRC64;
MASTASVSTS TASSELSSLS NNINNAADIS VIVIYFVVVM AVGVWAMLKT NRSTVGGFFL
AGRSMTWWPM GASLFASNIG SGHFVGLAGT GAASGIAVTA FESHSFALLL VLGWIFVPIY
IKAGVMTMPE YLKKRFGGKR LQIYLSILFL FICVILTISA DIFSGAIFIK LALGLNLYLA
ILILLAITAI FTITGGLASV IYTDTVQAVI MLVGSFILMV FAFVEVGGYE SFTEKFMNAI
PSVVEGDNLT INSRCYTPQP DSFHIFRDPV TGDIPWPGTA FGMPITALWY WCINQVIVQR
CLCGKNLSHV KAACILCGYL KLLPLFFMVM PGMISRILYT DMVACVVPSE CVKHCGVDVG
CTNYAYPMLV LKLMPPGLRG LMLSVMLASL MSSLTSVFNS ASTLFTIDLY TKIRKKASER
ELLIAGRLFV SVLIVTSILW VPIVEVSQGG QLVHYTEAIS SYLGPPIAAV FLVAVFCKRA
NEQGAFWGLM VGLVMGLIRM IAEFSYGTGS CLAPSSCPKI ICGVHYLYFA IILFFVCILV
ILGVSYLTKP IPDVHLHRLC WALRNSKEER IDLDAEDKEE NGADDRTEED QTEKPRGCLK
KTCDLFCGLQ RAEFKLTKVE EEALTDTTEK PFWRTVMNVN VILLLAVAAF FYGYFA
