S66A2_MOUSE
ID S66A2_MOUSE Reviewed; 271 AA.
AC Q80XM9; Q3U6E6; Q9CYA7; Q9D2D3; Q9D2V1; Q9D7G6;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Solute carrier family 66 member 2 {ECO:0000305};
DE AltName: Full=PQ-loop repeat-containing protein 1;
GN Name=Slc66a2; Synonyms=Pqlc1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4).
RC STRAIN=C57BL/6J;
RC TISSUE=Bone marrow, Cerebellum, Embryo, Liver, Testis, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Liver, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, and Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q80XM9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80XM9-2; Sequence=VSP_024146;
CC Name=3;
CC IsoId=Q80XM9-3; Sequence=VSP_024146, VSP_024149;
CC Name=4;
CC IsoId=Q80XM9-4; Sequence=VSP_024147, VSP_024148;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB31886.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK009256; BAB26173.1; -; mRNA.
DR EMBL; AK017854; BAB30976.1; -; mRNA.
DR EMBL; AK018759; BAB31391.1; -; mRNA.
DR EMBL; AK019858; BAB31886.1; ALT_FRAME; mRNA.
DR EMBL; AK047446; BAC33061.1; -; mRNA.
DR EMBL; AK153023; BAE31657.1; -; mRNA.
DR EMBL; AK153176; BAE31779.1; -; mRNA.
DR EMBL; BC008231; AAH08231.1; -; mRNA.
DR EMBL; BC029179; AAH29179.1; -; mRNA.
DR EMBL; BC043686; AAH43686.1; -; mRNA.
DR CCDS; CCDS29367.1; -. [Q80XM9-1]
DR CCDS; CCDS50334.1; -. [Q80XM9-2]
DR CCDS; CCDS89285.1; -. [Q80XM9-4]
DR RefSeq; NP_001157892.1; NM_001164420.1. [Q80XM9-2]
DR RefSeq; NP_001157893.1; NM_001164421.1. [Q80XM9-2]
DR RefSeq; NP_001157894.1; NM_001164422.1. [Q80XM9-2]
DR RefSeq; NP_001157895.1; NM_001164423.1. [Q80XM9-4]
DR RefSeq; NP_001157896.1; NM_001164424.1. [Q80XM9-4]
DR RefSeq; NP_080137.2; NM_025861.3. [Q80XM9-1]
DR RefSeq; XP_006526582.1; XM_006526519.1. [Q80XM9-1]
DR RefSeq; XP_006526583.1; XM_006526520.3. [Q80XM9-1]
DR AlphaFoldDB; Q80XM9; -.
DR STRING; 10090.ENSMUSP00000121890; -.
DR iPTMnet; Q80XM9; -.
DR PhosphoSitePlus; Q80XM9; -.
DR jPOST; Q80XM9; -.
DR MaxQB; Q80XM9; -.
DR PaxDb; Q80XM9; -.
DR PRIDE; Q80XM9; -.
DR ProteomicsDB; 291733; -. [Q80XM9-1]
DR ProteomicsDB; 291734; -. [Q80XM9-2]
DR ProteomicsDB; 291735; -. [Q80XM9-3]
DR ProteomicsDB; 291736; -. [Q80XM9-4]
DR Antibodypedia; 23501; 77 antibodies from 16 providers.
DR DNASU; 66943; -.
DR Ensembl; ENSMUST00000070135; ENSMUSP00000069986; ENSMUSG00000034006. [Q80XM9-2]
DR Ensembl; ENSMUST00000091798; ENSMUSP00000089402; ENSMUSG00000034006. [Q80XM9-1]
DR Ensembl; ENSMUST00000123750; ENSMUSP00000121890; ENSMUSG00000034006. [Q80XM9-2]
DR Ensembl; ENSMUST00000129043; ENSMUSP00000118670; ENSMUSG00000034006. [Q80XM9-2]
DR Ensembl; ENSMUST00000131780; ENSMUSP00000117166; ENSMUSG00000034006. [Q80XM9-1]
DR Ensembl; ENSMUST00000134545; ENSMUSP00000157740; ENSMUSG00000034006. [Q80XM9-4]
DR Ensembl; ENSMUST00000140594; ENSMUSP00000119083; ENSMUSG00000034006. [Q80XM9-1]
DR Ensembl; ENSMUST00000151677; ENSMUSP00000157569; ENSMUSG00000034006. [Q80XM9-4]
DR GeneID; 66943; -.
DR KEGG; mmu:66943; -.
DR UCSC; uc008fsu.2; mouse. [Q80XM9-1]
DR UCSC; uc008fsv.2; mouse. [Q80XM9-2]
DR UCSC; uc008fsw.2; mouse. [Q80XM9-4]
DR CTD; 80148; -.
DR MGI; MGI:1914193; Slc66a2.
DR VEuPathDB; HostDB:ENSMUSG00000034006; -.
DR eggNOG; KOG2913; Eukaryota.
DR GeneTree; ENSGT00390000002381; -.
DR HOGENOM; CLU_049047_2_0_1; -.
DR InParanoid; Q80XM9; -.
DR OMA; FKMWFFF; -.
DR OrthoDB; 1259147at2759; -.
DR PhylomeDB; Q80XM9; -.
DR TreeFam; TF313072; -.
DR BioGRID-ORCS; 66943; 1 hit in 71 CRISPR screens.
DR PRO; PR:Q80XM9; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q80XM9; protein.
DR Bgee; ENSMUSG00000034006; Expressed in granulocyte and 251 other tissues.
DR ExpressionAtlas; Q80XM9; baseline and differential.
DR Genevisible; Q80XM9; MM.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0045332; P:phospholipid translocation; IBA:GO_Central.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IBA:GO_Central.
DR InterPro; IPR006603; PQ-loop_rpt.
DR Pfam; PF04193; PQ-loop; 2.
DR SMART; SM00679; CTNS; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Phosphoprotein; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..271
FT /note="Solute carrier family 66 member 2"
FT /id="PRO_0000282431"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 14..80
FT /note="PQ-loop 1"
FT DOMAIN 178..233
FT /note="PQ-loop 2"
FT MOD_RES 110
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 113..130
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024146"
FT VAR_SEQ 131..134
FT /note="DFDP -> GMYY (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024147"
FT VAR_SEQ 135..271
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024148"
FT VAR_SEQ 143..271
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024149"
FT CONFLICT 33
FT /note="Y -> F (in Ref. 1; BAB31391)"
FT /evidence="ECO:0000305"
FT CONFLICT 42
FT /note="R -> T (in Ref. 1; BAB31391)"
FT /evidence="ECO:0000305"
FT CONFLICT 46
FT /note="A -> P (in Ref. 1; BAB31391)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="R -> P (in Ref. 1; BAB31886)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="D -> G (in Ref. 1; BAE31779)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 271 AA; 30580 MW; 5E2BB52892E43FB1 CRC64;
MEAEGLGWLL VPLHQLVSWV AAGAMVFGGV VPYIPQYRDI RRTQNADGFS THVCLVLLVA
NILRILFWFG RHFESPLLWQ SIVMILTMLL MLKLCTEVRV ANELNIKRRS FAATDSKDEE
LRVPPRRPYL DFDPHHFWHW SSFSDYVQCV LAFTGVAGYI TYLSIDSALF VETLGFLAVL
TEAMLGVPQL YRNYCHRSTE GMSLKMVLMW TSGDTFKTAY FLLNGAPLQF SVCGLLQVMV
DLVILGQAYA FAHHPQKPAA HAVHPASTKA L
