S6A12_CANLF
ID S6A12_CANLF Reviewed; 614 AA.
AC P27799;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Sodium- and chloride-dependent betaine transporter;
DE AltName: Full=Na(+)/Cl(-) betaine/GABA transporter;
DE AltName: Full=Solute carrier family 6 member 12;
GN Name=SLC6A12;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Cocker spaniel; TISSUE=Kidney;
RX PubMed=1370453; DOI=10.1016/s0021-9258(18)48543-2;
RA Yamauchi A., Uchida S., Kwon H.M., Preston A.S., Robey R.B.,
RA Garcia-Perez A., Burg M.B., Handler J.S.;
RT "Cloning of a Na(+)- and Cl(-)-dependent betaine transporter that is
RT regulated by hypertonicity.";
RL J. Biol. Chem. 267:649-652(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7862636; DOI=10.1073/pnas.92.4.1072;
RA Takenaka M., Bagnasco S.M., Preston A.S., Uchida S., Yamauchi A.,
RA Kwon H.M., Handler J.S.;
RT "The canine betaine gamma-amino-n-butyric acid transporter gene: diverse
RT mRNA isoforms are regulated by hypertonicity and are expressed in a tissue-
RT specific manner.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:1072-1076(1995).
RN [3]
RP INTERACTION WITH LIN7C.
RX PubMed=10710551; DOI=10.1152/ajprenal.2000.278.3.f464;
RA Straight S.W., Karnak D., Borg J.-P., Kamberov E., Dare H., Margolis B.,
RA Wade J.B.;
RT "mLin-7 is localized to the basolateral surface of renal epithelia via its
RT NH(2) terminus.";
RL Am. J. Physiol. 278:F464-F475(2000).
CC -!- FUNCTION: Transports betaine and GABA. May have a role in regulation of
CC GABAergic transmission in the brain through the reuptake of GABA into
CC presynaptic terminals, as well as in osmotic regulation.
CC -!- SUBUNIT: Interacts with LIN7C. {ECO:0000269|PubMed:10710551}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Kidney.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A12 subfamily. {ECO:0000305}.
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DR EMBL; M80403; AAA30877.1; -; mRNA.
DR EMBL; D42037; BAA22547.1; -; Genomic_DNA.
DR PIR; A41757; A41757.
DR RefSeq; NP_001003322.1; NM_001003322.3.
DR RefSeq; XP_005636741.1; XM_005636684.2.
DR RefSeq; XP_005636742.1; XM_005636685.2.
DR RefSeq; XP_005636743.1; XM_005636686.2.
DR RefSeq; XP_005636744.1; XM_005636687.2.
DR RefSeq; XP_005636745.1; XM_005636688.2.
DR RefSeq; XP_013963726.1; XM_014108251.1.
DR RefSeq; XP_013963727.1; XM_014108252.1.
DR AlphaFoldDB; P27799; -.
DR SMR; P27799; -.
DR MINT; P27799; -.
DR STRING; 9615.ENSCAFP00000023174; -.
DR iPTMnet; P27799; -.
DR PaxDb; P27799; -.
DR Ensembl; ENSCAFT00040046662; ENSCAFP00040040723; ENSCAFG00040024982.
DR Ensembl; ENSCAFT00845047370; ENSCAFP00845037173; ENSCAFG00845026822.
DR GeneID; 404013; -.
DR KEGG; cfa:404013; -.
DR CTD; 6539; -.
DR VEuPathDB; HostDB:ENSCAFG00845026822; -.
DR eggNOG; KOG3660; Eukaryota.
DR GeneTree; ENSGT00940000161075; -.
DR HOGENOM; CLU_006855_9_5_1; -.
DR InParanoid; P27799; -.
DR OMA; QDPQVWM; -.
DR OrthoDB; 250396at2759; -.
DR TreeFam; TF343812; -.
DR Reactome; R-CFA-352230; Amino acid transport across the plasma membrane.
DR Reactome; R-CFA-442660; Na+/Cl- dependent neurotransmitter transporters.
DR Reactome; R-CFA-71288; Creatine metabolism.
DR Reactome; R-CFA-888593; Reuptake of GABA.
DR Proteomes; UP000002254; Chromosome 27.
DR Bgee; ENSCAFG00000015756; Expressed in renal medulla and 44 other tissues.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005332; F:gamma-aminobutyric acid:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002983; Na/ntran_symport_betaine.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01198; BETTRANSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Glycoprotein; Membrane; Neurotransmitter transport;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..614
FT /note="Sodium- and chloride-dependent betaine transporter"
FT /id="PRO_0000214787"
FT TOPO_DOM 1..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..92
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..210
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..229
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..255
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..308
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..341
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..393
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..441
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 499..518
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 538..556
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 557..614
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 591..614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 157..166
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
SQ SEQUENCE 614 AA; 69292 MW; B19A9589843183CC CRC64;
MDRKVAVPED GPPVVSWLPE EGEKLDQEGE DQVKDRGQWT NKMEFVLSVA GEIIGLGNVW
RFPYLCYKNG GGAFFIPYFI FFFTCGIPVF FLEVALGQYT SQGSVTAWRK ICPLLQGIGL
ASVVIESYLN IYYIIILAWA LFYLFSSFTS ELPWTTCTNT WNTEHCMDFL NHSGARTATS
SENFTSPVME FWERRVLGIT SGIHDLGALR WELALCLLLA WLICYFCIWK GVKTTGKVVY
FTATFPYLML VILLIRGITL PGAYQGVIYY LKPDLLRLKD PQVWMDAGTQ IFFSFAICQG
CLTALGSYNK YHNNCYRDSI ALCFLNSATS FAAGFVVFSI LGFMAQEQGL PISEVAESGP
GLAFIAFPKA VTMMPLSQLW SCLFFIMLIF LGLDSQFVCV ECLVTASMDM FPSQLRKSGR
RELLILAIAV FCYLAGLFLV TEGGMYIFQL FDYYASSGIC LLFLAMFEVI CISWVYGADR
FYDNIEDMIG YRPWPLVKIS WLFLTPGLCL ATFLFSLSQY TPLKYNNIYV YPPWGYSIGW
FLALSSMICV PLFVIITLLK TRGSFKKRLR QLTTPDPSLP QPKQHLYLDG GTSQDCGPSP
TKEGLIVGEK ETHL
