S6A12_MOUSE
ID S6A12_MOUSE Reviewed; 614 AA.
AC P31651;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Sodium- and chloride-dependent betaine transporter;
DE AltName: Full=Na(+)/Cl(-) betaine/GABA transporter;
DE AltName: Full=Sodium- and chloride-dependent GABA transporter 2;
DE Short=GAT-2;
DE AltName: Full=Solute carrier family 6 member 12;
GN Name=Slc6a12; Synonyms=Gabt2, Gat-2, Gat2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=1517200; DOI=10.1016/s0021-9258(19)37067-x;
RA Lopez-Corcuera B., Liu Q.-R., Mandiyan S., Nelson H., Nelson N.;
RT "Expression of a mouse brain cDNA encoding novel gamma-aminobutyric acid
RT transporter.";
RL J. Biol. Chem. 267:17491-17493(1992).
CC -!- FUNCTION: Transports betaine and GABA. May have a role in regulation of
CC GABAergic transmission in the brain through the reuptake of GABA into
CC presynaptic terminals, as well as in osmotic regulation (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with LIN7C. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Brain, liver and kidney.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A12 subfamily. {ECO:0000305}.
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DR EMBL; M97632; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS90118.1; -.
DR PIR; A43390; A43390.
DR AlphaFoldDB; P31651; -.
DR SMR; P31651; -.
DR STRING; 10090.ENSMUSP00000032200; -.
DR BindingDB; P31651; -.
DR ChEMBL; CHEMBL4228; -.
DR DrugCentral; P31651; -.
DR GuidetoPHARMACOLOGY; 932; -.
DR GlyGen; P31651; 2 sites.
DR iPTMnet; P31651; -.
DR jPOST; P31651; -.
DR MaxQB; P31651; -.
DR PaxDb; P31651; -.
DR PRIDE; P31651; -.
DR ProteomicsDB; 253389; -.
DR MGI; MGI:95628; Slc6a12.
DR eggNOG; KOG3660; Eukaryota.
DR InParanoid; P31651; -.
DR Reactome; R-MMU-352230; Amino acid transport across the plasma membrane.
DR Reactome; R-MMU-442660; Na+/Cl- dependent neurotransmitter transporters.
DR Reactome; R-MMU-71288; Creatine metabolism.
DR Reactome; R-MMU-888593; Reuptake of GABA.
DR PRO; PR:P31651; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P31651; protein.
DR GO; GO:0016021; C:integral component of membrane; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005332; F:gamma-aminobutyric acid:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0008028; F:monocarboxylic acid transmembrane transporter activity; ISO:MGI.
DR GO; GO:0009992; P:cellular water homeostasis; ISO:MGI.
DR GO; GO:0015718; P:monocarboxylic acid transport; ISO:MGI.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002983; Na/ntran_symport_betaine.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01198; BETTRANSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Membrane; Neurotransmitter transport;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..614
FT /note="Sodium- and chloride-dependent betaine transporter"
FT /id="PRO_0000214789"
FT TOPO_DOM 1..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..92
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..210
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..229
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..255
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..308
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..341
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..393
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..441
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 499..518
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 538..556
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 557..614
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 574..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 585..602
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 157..166
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
SQ SEQUENCE 614 AA; 69614 MW; 9A6B49EA3503725B CRC64;
MDRKVAVHED GYPVVSWVPE EGEMMDQKGK DQVKDRGQWT NKMEFVLSVA GEIIGLGNVW
RFPYLCYKNG GGAFFIPYFI FFFSCGIPVF FLEVALGQYS SQGSVTAWRK ICPLLQGIGM
ASVVIESYLN IYYIIILAWA LFYLFSSFTW ELPWTTCTNS WNTEHCVDFL NHSSARGVSS
SENFTSPVME FWERRVLGIT SGIHDLGSLR WELALCLLLA WIICYFCIWK GVKSTGKVVY
FTATFPYLML IILLIRGVTL PGAYQGIVFY LKPDLLRLKD PQVWMDAGTQ IFFSFAICQG
CLTALGSYNK YHNNCYRDSI ALCFLNSATS FVAGFVVFSI LGFMSQEQGI PISEVAESGP
GLAFIAFPKA VTMMPLSQLW SCLFFIMLLF LGLDSQFVCM ECLVTASMDM FPQQLRKSGR
RDVLILAISV LCYLMGLLLV TEGGMYIFQL FDYYASSGIC LLFLSLFEVI CIGWVYGADR
FYDNVEDMIG YRPWPLVKIS WLFLTPGLCL ATFFFSLSKY TPLKYNNVYM YPSWGYSIGW
LLAFSSMACV PLFIIITFLK TQGSFKKRLR RLITPDPSLP QPGRRPPQDG SSAQNCSSSP
AKQELIAWEK ETHL
