S6A12_RAT
ID S6A12_RAT Reviewed; 614 AA.
AC P48056;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Sodium- and chloride-dependent betaine transporter;
DE AltName: Full=Na(+)/Cl(-) betaine/GABA transporter;
DE AltName: Full=Solute carrier family 6 member 12;
GN Name=Slc6a12;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=8865807; DOI=10.1016/0005-2736(96)00118-6;
RA Burnham C.E., Buerk B., Schmidt C., Bucuvalas J.C.;
RT "A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA
RT sequence and organ distribution.";
RL Biochim. Biophys. Acta 1284:4-8(1996).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Transports betaine and GABA. May have a role in regulation of
CC GABAergic transmission in the brain through the reuptake of GABA into
CC presynaptic terminals, as well as in osmotic regulation (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with LIN7C. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A12 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC52867.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U28927; AAC52867.1; ALT_INIT; mRNA.
DR RefSeq; NP_059031.1; NM_017335.1.
DR AlphaFoldDB; P48056; -.
DR SMR; P48056; -.
DR STRING; 10116.ENSRNOP00000018821; -.
DR BindingDB; P48056; -.
DR ChEMBL; CHEMBL2111478; -.
DR GlyGen; P48056; 2 sites.
DR iPTMnet; P48056; -.
DR PhosphoSitePlus; P48056; -.
DR PaxDb; P48056; -.
DR PRIDE; P48056; -.
DR GeneID; 50676; -.
DR KEGG; rno:50676; -.
DR UCSC; RGD:620255; rat.
DR CTD; 6539; -.
DR RGD; 620255; Slc6a12.
DR eggNOG; KOG3660; Eukaryota.
DR HOGENOM; CLU_006855_9_5_1; -.
DR InParanoid; P48056; -.
DR OrthoDB; 250396at2759; -.
DR PhylomeDB; P48056; -.
DR TreeFam; TF343812; -.
DR Reactome; R-RNO-352230; Amino acid transport across the plasma membrane.
DR Reactome; R-RNO-442660; Na+/Cl- dependent neurotransmitter transporters.
DR Reactome; R-RNO-71288; Creatine metabolism.
DR Reactome; R-RNO-888593; Reuptake of GABA.
DR PRO; PR:P48056; -.
DR Proteomes; UP000002494; Chromosome 4.
DR Bgee; ENSRNOG00000013547; Expressed in liver and 10 other tissues.
DR ExpressionAtlas; P48056; baseline and differential.
DR Genevisible; P48056; RN.
DR GO; GO:0016021; C:integral component of membrane; IDA:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005332; F:gamma-aminobutyric acid:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0008028; F:monocarboxylic acid transmembrane transporter activity; ISO:RGD.
DR GO; GO:0071474; P:cellular hyperosmotic response; IEP:RGD.
DR GO; GO:0009992; P:cellular water homeostasis; IDA:RGD.
DR GO; GO:0015718; P:monocarboxylic acid transport; ISO:RGD.
DR GO; GO:0006836; P:neurotransmitter transport; TAS:RGD.
DR GO; GO:0010033; P:response to organic substance; IEP:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002983; Na/ntran_symport_betaine.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01198; BETTRANSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Neurotransmitter transport; Phosphoprotein;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..614
FT /note="Sodium- and chloride-dependent betaine transporter"
FT /id="PRO_0000214791"
FT TOPO_DOM 1..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..92
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 138..210
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..229
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..255
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..308
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..341
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..393
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..441
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 499..518
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 538..556
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 557..614
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 574..600
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 584..600
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 586
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 614 AA; 69749 MW; 14C78DE5E1ED808B CRC64;
MDRKVTVHED GCPVVSWVPE EGEMMDQKDK DQVKDRGQWT NKMEFVLSVA GEIIGLGNVW
RFPYLCYKNG GGAFFIPYFI FFFSCGIPVF FLEVALGQYS SQGSVTAWRK ICPLLQGIGM
ASVVIESYLN IYYIIILAWA LFYLFSSFTW ELPWTTCTNS WNTEHCVDFL NYSSTRAASY
SENFTSPVME FWERRVLGIT SGIHDLGSLR WELALCLLLA WIICYFCIWK GVKSTGKVVY
FTATFPYLML IILLIRGVTL PGAYQGIVFY LKPDLLRLKD PQVWMDAGTQ IFFSFAICQG
CLTALGSYNK YHNNCYRDSI ALCFLNSATS FVAGFVVFSI LGFMAQEQGV PISEVAESGP
GLAFIAFPKA VTMMPLSQLW SCLFFLMLLF LGLDSQFVCM ECLVTASMDM FPQQLRKRGR
RELLILAVAI VCYLMGLLLV TEGGMYIFQL FDYYASSGIC LLFLSLFEVI CIGWVYGADR
FYDNVEDMIG YRPWPLVKIS WLFLTPGLCL ATFFFSLSKY TPLKYNNVYI YPSWGYSIGW
LLAFSSMACV PLFIIITLLK TQGSFKKRLQ RLITPDPSLP QPGRRSPQDG SSAQNCSTSP
VKQELIAWEK ETHL
