S6A13_BOVIN
ID S6A13_BOVIN Reviewed; 602 AA.
AC A5PJX7;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Sodium- and chloride-dependent GABA transporter 2;
DE Short=GAT-2;
DE AltName: Full=Solute carrier family 6 member 13;
GN Name=SLC6A13;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Fetal medulla;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mediates sodium- and chloride-dependent transport of gamma-
CC aminobutyric acid (GABA) (By similarity). Can also mediate transport of
CC beta-alanine, taurine and hypotaurine (By similarity).
CC {ECO:0000250|UniProtKB:P31649}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-aminobutanoate(out) + chloride(out) + 2 Na(+)(out) = 4-
CC aminobutanoate(in) + chloride(in) + 2 Na(+)(in);
CC Xref=Rhea:RHEA:70687, ChEBI:CHEBI:17996, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:59888; Evidence={ECO:0000250|UniProtKB:P31649};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70688;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + 2 Na(+)(out) + taurine(out) = chloride(in) + 2
CC Na(+)(in) + taurine(in); Xref=Rhea:RHEA:71223, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:507393;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71224;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-alanine(out) + chloride(out) + 2 Na(+)(out) = beta-
CC alanine(in) + chloride(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71247,
CC ChEBI:CHEBI:17996, ChEBI:CHEBI:29101, ChEBI:CHEBI:57966;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71248;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + hypotaurine(out) + 2 Na(+)(out) = chloride(in)
CC + hypotaurine(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71243,
CC ChEBI:CHEBI:17996, ChEBI:CHEBI:29101, ChEBI:CHEBI:57853;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71244;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P31649};
CC Multi-pass membrane protein {ECO:0000255}. Basolateral cell membrane
CC {ECO:0000250|UniProtKB:P31649}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A13 subfamily. {ECO:0000305}.
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DR EMBL; BC142276; AAI42277.1; -; mRNA.
DR RefSeq; NP_001092497.1; NM_001099027.1.
DR AlphaFoldDB; A5PJX7; -.
DR SMR; A5PJX7; -.
DR STRING; 9913.ENSBTAP00000019308; -.
DR PaxDb; A5PJX7; -.
DR Ensembl; ENSBTAT00000019308; ENSBTAP00000019308; ENSBTAG00000014525.
DR GeneID; 523784; -.
DR KEGG; bta:523784; -.
DR CTD; 6540; -.
DR VEuPathDB; HostDB:ENSBTAG00000014525; -.
DR VGNC; VGNC:34916; SLC6A13.
DR eggNOG; KOG3660; Eukaryota.
DR GeneTree; ENSGT00940000157478; -.
DR HOGENOM; CLU_006855_9_5_1; -.
DR InParanoid; A5PJX7; -.
DR OMA; TIWFVSR; -.
DR OrthoDB; 250396at2759; -.
DR TreeFam; TF343812; -.
DR Reactome; R-BTA-442660; Na+/Cl- dependent neurotransmitter transporters.
DR Reactome; R-BTA-888593; Reuptake of GABA.
DR Proteomes; UP000009136; Chromosome 5.
DR Bgee; ENSBTAG00000014525; Expressed in midbrain and 20 other tissues.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0005332; F:gamma-aminobutyric acid:sodium symporter activity; IBA:GO_Central.
DR GO; GO:0089718; P:amino acid import across plasma membrane; IEA:Ensembl.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0010940; P:positive regulation of necrotic cell death; IEA:Ensembl.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002981; Na/ntran_symport_GABA_GAT2.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01196; GAT2TRNSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Membrane;
KW Neurotransmitter transport; Phosphoprotein; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..602
FT /note="Sodium- and chloride-dependent GABA transporter 2"
FT /id="PRO_0000351503"
FT TOPO_DOM 1..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..206
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 528..548
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 549..602
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 587
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P31649"
FT MOD_RES 591
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P31649"
FT CARBOHYD 169
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 269
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 153..162
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
SQ SEQUENCE 602 AA; 67788 MW; 8B378732075B9F07 CRC64;
MDSRVSGTTS NGETKPVCPG LEKAAEDGAL QREQWSNKME FLLSVAGEII GLGNVWRFPY
LCYKNGGGAF FIPYLIFLFT CGIPVFLLET ALGQYTSQGG ITAWRKICPI FEGIGYASQM
IVTLLNIYYI IVLAWALFYL FSSFTIDLPW GSCRHDWNTE RCVEFQRTNG SLNATAENAT
SPVIEFWERR VLKISEGIQH LGALRWELAL CLLLAWVVCY FCIWKGVKST GKVVYFTATF
PYLMLVVLLI RGVTLPGAAQ GIQFYLYPNL TRLWDPQVWM DAGTQIFFSF AICLGCLTAL
GSYNKYHNNC YRDSIALCFL NSGTSFVAGF AIFSILGFMS QEQGVPISEV AESGPGLAFI
AYPRAVVMLP FSPLWACCFF FMVVLLGLDS QFVCVESLVT ALVDMYPRVF RKKNRREVLI
LGVSVTSFLV GLVMLTEGGM YVFQLFDYYA ASGMCLLFVA IFESFCVAWA YGAGRFYDNI
EDMIGYRPWP LIKYCWLFLT PAVCTATFLF SLIKYTPLTY NKKYKYPWWG DALGWLLALS
SMVCIPAWSC YKLSTLKGSF RERVRQLLCP AKDLPQGHRE GPSAPATPRT SLLILTELEP
HH
