S6A13_MACFA
ID S6A13_MACFA Reviewed; 609 AA.
AC Q2PG55;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 03-AUG-2022, entry version 52.
DE RecName: Full=Sodium- and chloride-dependent GABA transporter 2;
DE Short=GAT-2;
DE AltName: Full=Solute carrier family 6 member 13;
GN Name=SLC6A13; ORFNames=QbsB-10818;
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain stem;
RA Kobayashi M., Tanuma R., Hirata M., Osada N., Kusuda J., Sugano S.,
RA Hashimoto K.;
RT "Analysis of gene expression in cynomolgus monkey tissues by macaque cDNA
RT oligo-chips.";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mediates sodium- and chloride-dependent transport of gamma-
CC aminobutyric acid (GABA) (By similarity). Can also mediate transport of
CC beta-alanine, taurine and hypotaurine (By similarity).
CC {ECO:0000250|UniProtKB:P31649}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-aminobutanoate(out) + chloride(out) + 2 Na(+)(out) = 4-
CC aminobutanoate(in) + chloride(in) + 2 Na(+)(in);
CC Xref=Rhea:RHEA:70687, ChEBI:CHEBI:17996, ChEBI:CHEBI:29101,
CC ChEBI:CHEBI:59888; Evidence={ECO:0000250|UniProtKB:P31649};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70688;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + 2 Na(+)(out) + taurine(out) = chloride(in) + 2
CC Na(+)(in) + taurine(in); Xref=Rhea:RHEA:71223, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:507393;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71224;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-alanine(out) + chloride(out) + 2 Na(+)(out) = beta-
CC alanine(in) + chloride(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71247,
CC ChEBI:CHEBI:17996, ChEBI:CHEBI:29101, ChEBI:CHEBI:57966;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71248;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chloride(out) + hypotaurine(out) + 2 Na(+)(out) = chloride(in)
CC + hypotaurine(in) + 2 Na(+)(in); Xref=Rhea:RHEA:71243,
CC ChEBI:CHEBI:17996, ChEBI:CHEBI:29101, ChEBI:CHEBI:57853;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:71244;
CC Evidence={ECO:0000250|UniProtKB:P31649};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P31649};
CC Multi-pass membrane protein {ECO:0000255}. Basolateral cell membrane
CC {ECO:0000250|UniProtKB:P31649}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A13 subfamily. {ECO:0000305}.
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DR EMBL; AB220382; BAE72915.1; -; mRNA.
DR AlphaFoldDB; Q2PG55; -.
DR SMR; Q2PG55; -.
DR STRING; 9541.XP_005569772.1; -.
DR Ensembl; ENSMFAT00000029624; ENSMFAP00000001446; ENSMFAG00000038990.
DR eggNOG; KOG3660; Eukaryota.
DR GeneTree; ENSGT00940000157478; -.
DR Proteomes; UP000233100; Chromosome 11.
DR Bgee; ENSMFAG00000038990; Expressed in adult mammalian kidney and 12 other tissues.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0005332; F:gamma-aminobutyric acid:sodium symporter activity; IEA:Ensembl.
DR GO; GO:0089718; P:amino acid import across plasma membrane; IEA:Ensembl.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0010940; P:positive regulation of necrotic cell death; IEA:Ensembl.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002981; Na/ntran_symport_GABA_GAT2.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR01196; GAT2TRNSPORT.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Membrane;
KW Neurotransmitter transport; Phosphoprotein; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..609
FT /note="Sodium- and chloride-dependent GABA transporter 2"
FT /id="PRO_0000351504"
FT TOPO_DOM 1..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..206
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 528..548
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 549..609
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 594
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P31649"
FT MOD_RES 598
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P31649"
FT CARBOHYD 169
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 269
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 153..162
FT /evidence="ECO:0000250|UniProtKB:Q7K4Y6"
SQ SEQUENCE 609 AA; 68703 MW; 6A8B78D19CAB377E CRC64;
MDSRASGTAS NGETKPVYPV MEKEEEEGTL ERGHWNNKME FVLSVAGEII GLGNVWRFPY
LCYKNGGGAF FIPYLVFLFT CGVPVFLLET ALGQYTSQGG VTAWRKICPI FEGIGYASQM
IVILLNVYYI IVLAWALFYL FSSFTIDLPW GGCHHEWNTE HCVEFQKTNG SLNGTSENAT
SPVIEFWERR VLKISDGIQH LGALRWELAL CLLLAWVICY FCIWKGVKST GKVVYFTATF
PYLMLVVLLI RGVTLPGAAQ GIQFYLYPNL TRLWDPQVWM DAGTQIFFSF AICLGCLTAL
GSYNKYHNNC YRDCLALCFL NSGTSFVAGF AIFSILGFMS QEQGVPISEV AESGPGLAFI
AYPRAVVMLP FSPLWACCFF FMVVLLGLDS QFVCVESLVT ALVDMYPRVF RKKNRREVLI
LGVSVVSFLV GLVMLTEGGM YVFQLFDYYA ASGMCLLFVA IFESLCVAWV YGARRFYDNI
EDMIGYRPWP LIKYCWLFLT PAVCTATFLF SLIKYTPLTY NKKYTYPWWG DALGWLLALS
SMVCIPAWSL YRLGTLKGPF REPPPRSLQR IRQLMCPAED LPQRNPAGPS APATPRTSLL
RLTELESHC
