BET1_MOUSE
ID BET1_MOUSE Reviewed; 118 AA.
AC O35623;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=BET1 homolog;
DE Short=mBET1;
DE AltName: Full=Golgi vesicular membrane-trafficking protein p18;
GN Name=Bet1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9382863; DOI=10.1083/jcb.139.5.1157;
RA Zhang T., Wong S.H., Tang B.L., Xu Y., Peter F., Subramaniam V.N., Hong W.;
RT "The mammalian protein (rbet1) homologous to yeast Bet1p is primarily
RT associated with the pre-Golgi intermediate compartment and is involved in
RT vesicular transport from the endoplasmic reticulum to the Golgi
RT apparatus.";
RL J. Cell Biol. 139:1157-1168(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Required for vesicular transport from the ER to the Golgi
CC complex. Functions as a SNARE involved in the docking process of ER-
CC derived vesicles with the cis-Golgi membrane (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with STX17. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type IV membrane protein {ECO:0000250}. Golgi apparatus,
CC cis-Golgi network membrane {ECO:0000250}. Golgi apparatus membrane
CC {ECO:0000250}. Note=Concentrated most in the intermediate
CC compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2.
CC Greatly reduced in concentration at the trans end of the Golgi
CC apparatus (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the BET1 family. {ECO:0000305}.
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DR EMBL; AF007552; AAB62942.1; -; mRNA.
DR EMBL; BC005572; AAH05572.1; -; mRNA.
DR CCDS; CCDS19895.1; -.
DR RefSeq; NP_033878.1; NM_009748.2.
DR AlphaFoldDB; O35623; -.
DR SMR; O35623; -.
DR BioGRID; 198337; 1.
DR STRING; 10090.ENSMUSP00000044877; -.
DR iPTMnet; O35623; -.
DR PhosphoSitePlus; O35623; -.
DR SwissPalm; O35623; -.
DR EPD; O35623; -.
DR jPOST; O35623; -.
DR MaxQB; O35623; -.
DR PaxDb; O35623; -.
DR PeptideAtlas; O35623; -.
DR PRIDE; O35623; -.
DR ProteomicsDB; 273671; -.
DR TopDownProteomics; O35623; -.
DR DNASU; 12068; -.
DR Ensembl; ENSMUST00000049166; ENSMUSP00000044877; ENSMUSG00000032757.
DR GeneID; 12068; -.
DR KEGG; mmu:12068; -.
DR UCSC; uc009avk.1; mouse.
DR CTD; 10282; -.
DR MGI; MGI:1343104; Bet1.
DR VEuPathDB; HostDB:ENSMUSG00000032757; -.
DR eggNOG; KOG3385; Eukaryota.
DR GeneTree; ENSGT00940000163414; -.
DR HOGENOM; CLU_086133_2_1_1; -.
DR InParanoid; O35623; -.
DR OMA; DENERMT; -.
DR OrthoDB; 1450835at2759; -.
DR PhylomeDB; O35623; -.
DR TreeFam; TF323307; -.
DR Reactome; R-MMU-204005; COPII-mediated vesicle transport.
DR Reactome; R-MMU-6807878; COPI-mediated anterograde transport.
DR BioGRID-ORCS; 12068; 5 hits in 71 CRISPR screens.
DR ChiTaRS; Bet1; mouse.
DR PRO; PR:O35623; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; O35623; protein.
DR Bgee; ENSMUSG00000032757; Expressed in gastrula and 250 other tissues.
DR Genevisible; O35623; MM.
DR GO; GO:0005801; C:cis-Golgi network; ISO:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000137; C:Golgi cis cisterna; ISO:MGI.
DR GO; GO:0031985; C:Golgi cisterna; ISO:MGI.
DR GO; GO:0000139; C:Golgi membrane; IDA:MGI.
DR GO; GO:0000138; C:Golgi trans cisterna; IBA:GO_Central.
DR GO; GO:0030173; C:integral component of Golgi membrane; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; ISO:MGI.
DR GO; GO:0031201; C:SNARE complex; ISO:MGI.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0019905; F:syntaxin binding; ISO:MGI.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISO:MGI.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0048280; P:vesicle fusion with Golgi apparatus; ISO:MGI.
DR GO; GO:0016192; P:vesicle-mediated transport; TAS:MGI.
DR CDD; cd15853; SNARE_Bet1; 1.
DR InterPro; IPR039897; BET1.
DR InterPro; IPR039899; BET1_SNARE.
DR InterPro; IPR000727; T_SNARE_dom.
DR PANTHER; PTHR12791; PTHR12791; 1.
DR SMART; SM00397; t_SNARE; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus;
KW Membrane; Phosphoprotein; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..118
FT /note="BET1 homolog"
FT /id="PRO_0000206885"
FT TOPO_DOM 1..94
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..118
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT DOMAIN 26..88
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT MOD_RES 50
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
SQ SEQUENCE 118 AA; 13274 MW; CB81C325119BA152 CRC64;
MRRAGLGDGA PPGSYGNYGY ANTGYNACEE ENDRLTESLR SKVTAIKSLS IEIGHEVKNQ
NKLLAEMDSQ FDSTTGFLGK TMGRLKILSR GSQTKLLCYM MLFSLFVFFV IYWIIKLR
