S6A15_RAT
ID S6A15_RAT Reviewed; 729 AA.
AC Q08469; Q63838;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Sodium-dependent neutral amino acid transporter B(0)AT2;
DE AltName: Full=Solute carrier family 6 member 15;
DE AltName: Full=Transporter v7-3;
DE AltName: Full=sodium- and chloride-dependent neurotransmitter transporter NTT73;
GN Name=Slc6a15; Synonyms=Ntt73;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=1363329; DOI=10.1016/0169-328x(92)90246-8;
RA Uhl G.R., Kitayama S., Gregor P., Nanthakumar E., Persico A.M., Shimada S.;
RT "Neurotransmitter transporter family cDNAs in a rat midbrain library:
RT 'orphan transporters' suggest sizable structural variations.";
RL Brain Res. Mol. Brain Res. 16:353-359(1992).
RN [2]
RP TISSUE SPECIFICITY.
RX PubMed=8738154; DOI=10.1016/0169-328x(95)00298-7;
RA Inoue K., Sato K., Tohyama M., Shimada S., Uhl G.R.;
RT "Widespread brain distribution of mRNA encoding the orphan neurotransmitter
RT transporter v7-3.";
RL Brain Res. Mol. Brain Res. 37:217-223(1996).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-699 AND SER-701, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Functions as a sodium-dependent neutral amino acid
CC transporter. Exhibits preference for methionine and for the branched-
CC chain amino acids, particularly leucine, valine and isoleucine.
CC Mediates the saturable, pH-sensitive and electrogenic cotransport of
CC proline and sodium ions with a stoichiometry of 1:1. May have a role as
CC transporter for neurotransmitter precursors into neurons. In contrast
CC to other members of the neurotransmitter transporter family, does not
CC appear to be chloride-dependent (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Widely distributed in the central nervous system,
CC including the olfactory bulb, the hypothalamus, the cerebral cortex,
CC the hippocampus, and the cerebellum. In addition, intense expression is
CC found in the motor nuclei including the oculomotor nucleus, abducens
CC nucleus, trigeminal motor nucleus, facial nucleus, hypoglossal nucleus
CC and ventral horn of spinal cord. Intense hybridization signals are also
CC observed in the nuclei containing monoaminergic neurons, such as locus
CC coeruleus, the substantia nigra pars compacta, the ventral tegmental
CC area, the dorsal raphe nucleus and the median raphe nucleus.
CC {ECO:0000269|PubMed:8738154}.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A15 subfamily. {ECO:0000305}.
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DR EMBL; L22022; AAA41729.1; -; mRNA.
DR EMBL; S56968; AAB25532.1; -; mRNA.
DR PIR; I65413; I65413.
DR RefSeq; NP_758824.1; NM_172321.1.
DR AlphaFoldDB; Q08469; -.
DR SMR; Q08469; -.
DR STRING; 10116.ENSRNOP00000032996; -.
DR GlyGen; Q08469; 3 sites.
DR iPTMnet; Q08469; -.
DR PhosphoSitePlus; Q08469; -.
DR PaxDb; Q08469; -.
DR Ensembl; ENSRNOT00000029208; ENSRNOP00000032996; ENSRNOG00000027468.
DR GeneID; 282712; -.
DR KEGG; rno:282712; -.
DR UCSC; RGD:628664; rat.
DR CTD; 55117; -.
DR RGD; 628664; Slc6a15.
DR eggNOG; KOG3659; Eukaryota.
DR GeneTree; ENSGT00940000157277; -.
DR HOGENOM; CLU_006855_7_1_1; -.
DR InParanoid; Q08469; -.
DR OMA; VVFCLRY; -.
DR OrthoDB; 250396at2759; -.
DR PhylomeDB; Q08469; -.
DR TreeFam; TF352709; -.
DR Reactome; R-RNO-352230; Amino acid transport across the plasma membrane.
DR Reactome; R-RNO-442660; Na+/Cl- dependent neurotransmitter transporters.
DR PRO; PR:Q08469; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Bgee; ENSRNOG00000027468; Expressed in Ammon's horn and 9 other tissues.
DR Genevisible; Q08469; RN.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005298; F:proline:sodium symporter activity; ISS:UniProtKB.
DR GO; GO:0015820; P:leucine transport; ISO:RGD.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0015804; P:neutral amino acid transport; ISS:UniProtKB.
DR GO; GO:0015824; P:proline transport; ISO:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR CDD; cd11522; SLC6sbd_SBAT1; 1.
DR InterPro; IPR042934; B(0)AT2.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002438; Neutral_aa_SLC6.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR00176; NANEUSMPORT.
DR PRINTS; PR01206; ORPHTRNSPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Glycoprotein; Ion transport; Membrane;
KW Neurotransmitter transport; Phosphoprotein; Reference proteome; Sodium;
KW Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..729
FT /note="Sodium-dependent neutral amino acid transporter
FT B(0)AT2"
FT /id="PRO_0000214800"
FT TOPO_DOM 1..69
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..117
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..225
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..244
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..270
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..323
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..356
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 357..452
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..472
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..514
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..550
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 571..592
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 620..642
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 643..729
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 25
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H2J7"
FT MOD_RES 55
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H2J7"
FT MOD_RES 687
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H2J7"
FT MOD_RES 699
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 701
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CARBOHYD 187
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 383
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 394
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 1..3
FT /note="MPK -> IP (in Ref. 1; AAB25532)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 729 AA; 81596 MW; ECDDC6403741B53E CRC64;
MPKNSKVVKR DLDDDVIESV KDLLSNEDSV EDVSKKSELI VDVQEEKDTD AEDGSEVDDE
RPAWNSKLQY ILAQVGFSVG LGNVWRFPYL CQKNGGGAYL LPYLILLLVI GIPLFFLELS
VGQRIRRGSI GVWNYISPKL GGIGFASCVV CYFVALYYNV IIGWTLFYFS QSFQQPLPWD
QCPLVKNASH TYIEPECEKS SATTYYWYRE ALAISSSISE SGGLNWKMTG CLLAAWVMVC
LAMIKGIQSS GKIMYFSSLF PYVVLICFLI RSLLLNGSID GIRHMFTPKL EMMLEPKVWR
EAATQVFFAL GLGFGGVIAF SSYNKRDNNC HFDAVLVSFI NFFTSVLATL VVFAVLGFKA
NIVNEKCISQ NSEMILKLLK TGNVSWDVIP RHINLSAVTA EDYHVVYDII QKVKEEEFAV
LHLKACQIED ELNKAVQGTG LAFIAFTEAM THFPASPFWS VMFFLMLINL GLGSMFGTIE
GIITPVVDTF KVRKEILTVI CCLLAFCIGL MFVQRSGNYF VTMFDDYSAT LPLLIVVILE
NIAVSFVYGI DKFLEDLTDM LGFAPSKYYY YMWKYISPLM LVTLLIASIV NMGLSPPGYN
AWIKEKASEE FLSYPMWGMV VCFSLMVLAI LPVPVVFVIR RCNLIDDSSG NLASVTYKRG
RVLKEPVNLD GDDASLIHGK IPSEMSSPNF GKNIYRKQSG SPTLDTAPNG RYGIGYLMAD
MPDMPESDL
