S6A16_HUMAN
ID S6A16_HUMAN Reviewed; 736 AA.
AC Q9GZN6; Q8IYV4; Q9Y5I9;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Orphan sodium- and chloride-dependent neurotransmitter transporter NTT5;
DE AltName: Full=Solute carrier family 6 member 16;
GN Name=SLC6A16; Synonyms=NTT5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RA Sloan J.L., Mager S.;
RT "Cloning of a new member of the neurotransmitter transporter family.";
RL Abstr. - Soc. Neurosci. 24:1606-1606(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=11112352; DOI=10.1006/geno.2000.6387;
RA Farmer M.K., Robbins M.J., Medhurst A.D., Campbell D.A., Ellington K.,
RA Duckworth M., Brown A.M., Middlemiss D.N., Price G.W., Pangalos M.N.;
RT "Cloning and characterization of human NTT5 and v7-3: two orphan
RT transporters of the Na(+)/Cl(-)-dependent neurotransmitter transporter gene
RT family.";
RL Genomics 70:241-252(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057824; DOI=10.1038/nature02399;
RA Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E.,
RA Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A.,
RA Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S.,
RA Carrano A.V., Caoile C., Chan Y.M., Christensen M., Cleland C.A.,
RA Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J.,
RA Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M.,
RA Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W.,
RA Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V.,
RA Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D.,
RA McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I.,
RA Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L.,
RA Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A.,
RA She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M.,
RA Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J.,
RA Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E.,
RA Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M.,
RA Rubin E.M., Lucas S.M.;
RT "The DNA sequence and biology of human chromosome 19.";
RL Nature 428:529-535(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP VARIANT LYS-236.
RX PubMed=21248752; DOI=10.1038/nature09639;
RA Varela I., Tarpey P., Raine K., Huang D., Ong C.K., Stephens P., Davies H.,
RA Jones D., Lin M.L., Teague J., Bignell G., Butler A., Cho J.,
RA Dalgliesh G.L., Galappaththige D., Greenman C., Hardy C., Jia M.,
RA Latimer C., Lau K.W., Marshall J., McLaren S., Menzies A., Mudie L.,
RA Stebbings L., Largaespada D.A., Wessels L.F.A., Richard S., Kahnoski R.J.,
RA Anema J., Tuveson D.A., Perez-Mancera P.A., Mustonen V., Fischer A.,
RA Adams D.J., Rust A., Chan-On W., Subimerb C., Dykema K., Furge K.,
RA Campbell P.J., Teh B.T., Stratton M.R., Futreal P.A.;
RT "Exome sequencing identifies frequent mutation of the SWI/SNF complex gene
RT PBRM1 in renal carcinoma.";
RL Nature 469:539-542(2011).
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9GZN6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9GZN6-2; Sequence=VSP_056317;
CC -!- TISSUE SPECIFICITY: Highly expressed in peripheral tissues,
CC particularly in testis, pancreas, and prostate.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A16 subfamily. {ECO:0000305}.
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DR EMBL; AF151977; AAD38044.1; -; mRNA.
DR EMBL; AF265578; AAG41362.1; -; mRNA.
DR EMBL; AL136856; CAB66790.1; -; mRNA.
DR EMBL; AC011450; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC034948; AAH34948.1; -; mRNA.
DR CCDS; CCDS42590.1; -. [Q9GZN6-1]
DR RefSeq; NP_054756.2; NM_014037.2. [Q9GZN6-1]
DR RefSeq; XP_005258877.1; XM_005258820.2. [Q9GZN6-1]
DR RefSeq; XP_006723231.1; XM_006723168.3. [Q9GZN6-1]
DR AlphaFoldDB; Q9GZN6; -.
DR SMR; Q9GZN6; -.
DR STRING; 9606.ENSP00000338627; -.
DR TCDB; 2.A.22.6.5; the neurotransmitter:sodium symporter (nss) family.
DR GlyGen; Q9GZN6; 1 site.
DR iPTMnet; Q9GZN6; -.
DR PhosphoSitePlus; Q9GZN6; -.
DR BioMuta; SLC6A16; -.
DR DMDM; 18202928; -.
DR MassIVE; Q9GZN6; -.
DR PaxDb; Q9GZN6; -.
DR PeptideAtlas; Q9GZN6; -.
DR PRIDE; Q9GZN6; -.
DR ProteomicsDB; 71245; -.
DR ProteomicsDB; 80098; -. [Q9GZN6-1]
DR Antibodypedia; 31965; 84 antibodies from 20 providers.
DR DNASU; 28968; -.
DR Ensembl; ENST00000335875.9; ENSP00000338627.3; ENSG00000063127.16. [Q9GZN6-1]
DR Ensembl; ENST00000454748.7; ENSP00000404022.2; ENSG00000063127.16. [Q9GZN6-2]
DR GeneID; 28968; -.
DR KEGG; hsa:28968; -.
DR MANE-Select; ENST00000335875.9; ENSP00000338627.3; NM_014037.3; NP_054756.2.
DR UCSC; uc002pmz.4; human. [Q9GZN6-1]
DR CTD; 28968; -.
DR DisGeNET; 28968; -.
DR GeneCards; SLC6A16; -.
DR HGNC; HGNC:13622; SLC6A16.
DR HPA; ENSG00000063127; Tissue enriched (testis).
DR MIM; 607972; gene.
DR neXtProt; NX_Q9GZN6; -.
DR OpenTargets; ENSG00000063127; -.
DR PharmGKB; PA37800; -.
DR VEuPathDB; HostDB:ENSG00000063127; -.
DR eggNOG; KOG3659; Eukaryota.
DR GeneTree; ENSGT00940000163283; -.
DR HOGENOM; CLU_006855_7_2_1; -.
DR InParanoid; Q9GZN6; -.
DR OMA; WATIITH; -.
DR OrthoDB; 250396at2759; -.
DR PhylomeDB; Q9GZN6; -.
DR TreeFam; TF352709; -.
DR PathwayCommons; Q9GZN6; -.
DR BioGRID-ORCS; 28968; 14 hits in 1075 CRISPR screens.
DR ChiTaRS; SLC6A16; human.
DR GenomeRNAi; 28968; -.
DR Pharos; Q9GZN6; Tdark.
DR PRO; PR:Q9GZN6; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9GZN6; protein.
DR Bgee; ENSG00000063127; Expressed in left testis and 106 other tissues.
DR ExpressionAtlas; Q9GZN6; baseline and differential.
DR Genevisible; Q9GZN6; HS.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005326; F:neurotransmitter transmembrane transporter activity; NAS:UniProtKB.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006836; P:neurotransmitter transport; NAS:UniProtKB.
DR GO; GO:0071705; P:nitrogen compound transport; IEA:UniProt.
DR GO; GO:0071702; P:organic substance transport; IEA:UniProt.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR00176; NANEUSMPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Neurotransmitter transport;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..736
FT /note="Orphan sodium- and chloride-dependent
FT neurotransmitter transporter NTT5"
FT /id="PRO_0000214801"
FT TOPO_DOM 1..138
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..219
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 220..263
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 404..495
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 534..554
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 568..588
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 609..629
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 659..679
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 680..736
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 229
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 648..736
FT /note="SKEVLRPYPPWALLLMITLFAIVILPIPAYFVYCRIHRIPFRPKSGDGPMTA
FT STSLPLSHQLTPSKEVQKEEILQVDETKYPSTCNVTS -> VSLPQDPEPLWE (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_056317"
FT VARIANT 108
FT /note="S -> R (in dbSNP:rs35860981)"
FT /id="VAR_052067"
FT VARIANT 236
FT /note="E -> K (found in a renal cell carcinoma sample;
FT somatic mutation)"
FT /evidence="ECO:0000269|PubMed:21248752"
FT /id="VAR_064753"
FT CONFLICT 181
FT /note="W -> R (in Ref. 1; AAD38044)"
FT /evidence="ECO:0000305"
FT CONFLICT 307
FT /note="G -> GG (in Ref. 1; AAD38044)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 736 AA; 82200 MW; E21EA16515446C35 CRC64;
MKTEAQPSTS LLANTSWTGT VISDSVPGSQ TWEDKGSLTR SATSWTSEAQ VSAARVAEAQ
ARTSQPKQIS VLEALTASAL NQKPTHEKVQ MTEKKESEVL LARPFWSSKT EYILAQVGFS
MKPSCLWRFA YLWLNSGGCS FAAIYIFMLF LVGVPLLFLE MAAGQSMRQG GMGVWKIIAP
WIGGVGYSSF MVCFILGLYF NVVNSWIIFY MSQSFQFPVP WEKCPLTMNS SGFDPECERT
TPSIYFWYQQ ALKASDRIED GGSPVYSLVL PFFLCWCLVG AFMINGLKST GKVIYVLVLL
PCFIIVGFFI RTLLLEGAKF GLQQLVVAKI SDVYNMSVWS LAGGQVLSNT GIGLGSVASL
ASYMPQSNNC LSDAFLVSVI NLLTLLVFTS FNFCVLGFWA TVITHRCCER NAEILLKLIN
LGKLPPDAKP PVNLLYNPTS IYNAWLSGLP QHIKSMVLRE VTECNIETQF LKASEGPKFA
FLSFVEAMSF LPPSVFWSFI FFLMLLAMGL SSAIGIMQGI ITPLQDTFSF FRKHTKLLIV
GVFLLMFVCG LFFTRPSGSY FIRLLSDYWI VFPIIVVVVF ETMAVSWAYG ARRFLADLTI
LLGHPISPIF GWLWPHLCPV VLLIIFVTMM VHLCMKPITY MSWDSSTSKE VLRPYPPWAL
LLMITLFAIV ILPIPAYFVY CRIHRIPFRP KSGDGPMTAS TSLPLSHQLT PSKEVQKEEI
LQVDETKYPS TCNVTS
