S6A18_RAT
ID S6A18_RAT Reviewed; 615 AA.
AC Q62687;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Sodium-dependent neutral amino acid transporter B(0)AT3;
DE AltName: Full=Renal osmotic stress-induced Na-Cl organic solute cotransporter;
DE Short=ROSIT;
DE AltName: Full=Sodium- and chloride-dependent transporter XTRP2;
DE AltName: Full=Solute carrier family 6 member 18;
DE AltName: Full=System B(0) neutral amino acid transporter AT3;
GN Name=Slc6a18; Synonyms=Xtrp2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=Sprague-Dawley {ECO:0000269|PubMed:7943364};
RC TISSUE=Kidney cortex {ECO:0000269|PubMed:7943364};
RX PubMed=7943364; DOI=10.1152/ajprenal.1994.267.4.f688;
RA Wasserman J.C., Delpire E., Tonidandel W., Kojima R., Gullans S.R.;
RT "Molecular characterization of ROSIT, a renal osmotic stress-induced Na(+)-
RT Cl(-)-organic solute cotransporter.";
RL Am. J. Physiol. 267:F688-F694(1994).
CC -!- FUNCTION: Functions as a sodium and chloride-dependent neutral amino
CC acid transporter in kidneys. Requires CLTRN for surface expression and
CC for its amino acid transporter activity.
CC {ECO:0000250|UniProtKB:O88576}.
CC -!- SUBUNIT: Interacts with CLTRN; this interaction regulates the
CC trafficking of SLC6A18 to the cell membrane and its amino acid
CC transporter activity. {ECO:0000250|UniProtKB:O88576}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane
CC {ECO:0000250|UniProtKB:O88576}; Multi-pass membrane protein
CC {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:O88576}; Multi-pass
CC membrane protein {ECO:0000250|UniProtKB:O88576}. Note=In kidneys
CC localizes to the apical membrane in distal segments of the proximal
CC tubule. {ECO:0000250|UniProtKB:O88576}.
CC -!- TISSUE SPECIFICITY: Kidney-specific expression.
CC {ECO:0000269|PubMed:7943364}.
CC -!- INDUCTION: By renal osmotic stress. {ECO:0000269|PubMed:7943364}.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A18 subfamily. {ECO:0000305}.
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DR EMBL; U12973; AAC13771.1; -; mRNA.
DR RefSeq; NP_058859.1; NM_017163.1.
DR AlphaFoldDB; Q62687; -.
DR SMR; Q62687; -.
DR STRING; 10116.ENSRNOP00000022346; -.
DR GlyGen; Q62687; 3 sites.
DR iPTMnet; Q62687; -.
DR PhosphoSitePlus; Q62687; -.
DR PaxDb; Q62687; -.
DR PRIDE; Q62687; -.
DR GeneID; 29323; -.
DR KEGG; rno:29323; -.
DR UCSC; RGD:69352; rat.
DR CTD; 348932; -.
DR RGD; 69352; Slc6a18.
DR eggNOG; KOG3659; Eukaryota.
DR InParanoid; Q62687; -.
DR OrthoDB; 547281at2759; -.
DR PhylomeDB; Q62687; -.
DR Reactome; R-RNO-352230; Amino acid transport across the plasma membrane.
DR Reactome; R-RNO-442660; Na+/Cl- dependent neurotransmitter transporters.
DR PRO; PR:Q62687; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0031526; C:brush border membrane; ISO:RGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0015175; F:neutral amino acid transmembrane transporter activity; ISO:RGD.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0003333; P:amino acid transmembrane transport; ISO:RGD.
DR GO; GO:0006972; P:hyperosmotic response; IEP:RGD.
DR GO; GO:0006836; P:neurotransmitter transport; IEA:UniProtKB-KW.
DR GO; GO:0006970; P:response to osmotic stress; IEP:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR CDD; cd11517; SLC6sbd_B0AT3; 1.
DR InterPro; IPR042701; B0AT3_SLC6sbd.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002438; Neutral_aa_SLC6.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR00176; NANEUSMPORT.
DR PRINTS; PR01206; ORPHTRNSPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell membrane; Glycoprotein; Membrane;
KW Neurotransmitter transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..615
FT /note="Sodium-dependent neutral amino acid transporter
FT B(0)AT3"
FT /id="PRO_0000214808"
FT TOPO_DOM 1..26
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..52
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..105
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..177
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..206
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..255
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..290
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..397
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 419..442
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 443..463
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 464..472
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 473..493
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 494..520
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 521..541
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 542..571
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 572..592
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 593..615
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 143
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000305"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000305"
FT CARBOHYD 353
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000305"
SQ SEQUENCE 615 AA; 69556 MW; F41E7DE1DC276918 CRC64;
MAQASGMDPL VDIEDERPKW DNKLQYLLSC IGFAVGLGNI WRFPYLCHTH GGGAFLIPYF
IALVFEGIPL FYIELAIGQR LRRGSIGVWK TISPYLGGVG LGCFSVSFLV SLYYNTILLW
VLWFFLNSFQ HPLPWSTCPL DLNRTGFVQE CQSSGTVSYF WYRQTLNITS DISNTGTIQW
KLFLCLVACW TTVYLCVIRG IESTGKVIYF TALFPYLVLT IFLIRGLTLP GATEGLTYLF
TPNMKILQNS RVWLDAATQI FFSLSLAFGG HIAFASYNQP RNNCEKDAVT IALVNSMTSL
YASITIFSIM GFKASNDYGR CLDRNILSLI NEFDFPELSI SRDEYPSVLM YLNATQPERV
ARLPLKTCHL EDFLDKSASG PGLAFIVFTE AVLHMPGASV WSVLFFGMLF TLGLSSMFGN
MEGVITPLFD MGILPKGVPK ETMTGVVCFI CFLSAICFTL QSGSYWLEIF DSFAASLNLI
IFAFMEVVGV IHVYGIKRFC DDIEWMTGRR PSLYWQVTWR VVSPMLLFGI FLSYIVLLAQ
SSPSYKAWNP QYEHFPSREE KLYPGWVQVT CVLLSFLPSL WVPGIALAQL LFQYRQRWKN
THLESALKPQ ESRGC
