ID   BET1_RAT                Reviewed;         118 AA.
AC   Q62896;
DT   17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=BET1 homolog;
DE            Short=rBET1;
DE   AltName: Full=Golgi vesicular membrane-trafficking protein p18;
GN   Name=Bet1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Liver;
RX   PubMed=8621431; DOI=10.1074/jbc.271.10.5671;
RA   Hay J.C., Hirling H., Scheller R.H.;
RT   "Mammalian vesicle trafficking proteins of the endoplasmic reticulum and
RT   Golgi apparatus.";
RL   J. Biol. Chem. 271:5671-5679(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=9382863; DOI=10.1083/jcb.139.5.1157;
RA   Zhang T., Wong S.H., Tang B.L., Xu Y., Peter F., Subramaniam V.N., Hong W.;
RT   "The mammalian protein (rbet1) homologous to yeast Bet1p is primarily
RT   associated with the pre-Golgi intermediate compartment and is involved in
RT   vesicular transport from the endoplasmic reticulum to the Golgi
RT   apparatus.";
RL   J. Cell Biol. 139:1157-1168(1997).
RN   [4]
RP   INTERACTION WITH STX17.
RX   PubMed=10930465; DOI=10.1091/mbc.11.8.2719;
RA   Steegmaier M., Oorschot V., Klumperman J., Scheller R.H.;
RT   "Syntaxin 17 is abundant in steroidogenic cells and implicated in smooth
RT   endoplasmic reticulum membrane dynamics.";
RL   Mol. Biol. Cell 11:2719-2731(2000).
RN   [5]
RP   SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=14742712; DOI=10.1091/mbc.e03-08-0625;
RA   Volchuk A., Ravazzola M., Perrelet A., Eng W.S., Di Liberto M.,
RA   Varlamov O., Fukasawa M., Engel T., Sollner T.H., Rothman J.E., Orci L.;
RT   "Countercurrent distribution of two distinct SNARE complexes mediating
RT   transport within the Golgi stack.";
RL   Mol. Biol. Cell 15:1506-1518(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Required for vesicular transport from the ER to the Golgi
CC       complex. Functions as a SNARE involved in the docking process of ER-
CC       derived vesicles with the cis-Golgi membrane.
CC       {ECO:0000269|PubMed:14742712}.
CC   -!- SUBUNIT: Interacts with STX17. {ECO:0000305|PubMed:10930465}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:14742712}; Single-pass type IV membrane protein
CC       {ECO:0000269|PubMed:14742712}. Golgi apparatus, cis-Golgi network
CC       membrane {ECO:0000269|PubMed:14742712}. Golgi apparatus membrane
CC       {ECO:0000269|PubMed:14742712}. Note=Concentrated most in the
CC       intermediate compartment/cis-Golgi network and the cis-Golgi cisternae
CC       1 and 2. Greatly reduced in concentration at the trans end of the Golgi
CC       apparatus.
CC   -!- SIMILARITY: Belongs to the BET1 family. {ECO:0000305}.
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DR   EMBL; U42755; AAC52441.1; -; mRNA.
DR   EMBL; BC062408; AAH62408.1; -; mRNA.
DR   RefSeq; NP_062124.1; NM_019251.2.
DR   AlphaFoldDB; Q62896; -.
DR   SMR; Q62896; -.
DR   CORUM; Q62896; -.
DR   IntAct; Q62896; 5.
DR   STRING; 10116.ENSRNOP00000014810; -.
DR   iPTMnet; Q62896; -.
DR   PhosphoSitePlus; Q62896; -.
DR   PaxDb; Q62896; -.
DR   PRIDE; Q62896; -.
DR   GeneID; 29631; -.
DR   KEGG; rno:29631; -.
DR   UCSC; RGD:2203; rat.
DR   CTD; 10282; -.
DR   RGD; 2203; Bet1.
DR   VEuPathDB; HostDB:ENSRNOG00000011008; -.
DR   eggNOG; KOG3385; Eukaryota.
DR   HOGENOM; CLU_086133_2_1_1; -.
DR   InParanoid; Q62896; -.
DR   OMA; DENERMT; -.
DR   OrthoDB; 1450835at2759; -.
DR   PhylomeDB; Q62896; -.
DR   TreeFam; TF323307; -.
DR   Reactome; R-RNO-204005; COPII-mediated vesicle transport.
DR   Reactome; R-RNO-6807878; COPI-mediated anterograde transport.
DR   PRO; PR:Q62896; -.
DR   Proteomes; UP000002494; Chromosome 4.
DR   Bgee; ENSRNOG00000011008; Expressed in pancreas and 20 other tissues.
DR   Genevisible; Q62896; RN.
DR   GO; GO:0005801; C:cis-Golgi network; IDA:RGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000137; C:Golgi cis cisterna; IDA:RGD.
DR   GO; GO:0031985; C:Golgi cisterna; IDA:RGD.
DR   GO; GO:0000139; C:Golgi membrane; ISO:RGD.
DR   GO; GO:0000138; C:Golgi trans cisterna; IBA:GO_Central.
DR   GO; GO:0030173; C:integral component of Golgi membrane; IEA:InterPro.
DR   GO; GO:0016021; C:integral component of membrane; IDA:RGD.
DR   GO; GO:0031201; C:SNARE complex; IDA:RGD.
DR   GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR   GO; GO:0019905; F:syntaxin binding; IPI:UniProtKB.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IDA:MGI.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0048280; P:vesicle fusion with Golgi apparatus; IDA:RGD.
DR   CDD; cd15853; SNARE_Bet1; 1.
DR   InterPro; IPR039897; BET1.
DR   InterPro; IPR039899; BET1_SNARE.
DR   InterPro; IPR000727; T_SNARE_dom.
DR   PANTHER; PTHR12791; PTHR12791; 1.
DR   SMART; SM00397; t_SNARE; 1.
DR   PROSITE; PS50192; T_SNARE; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus;
KW   Membrane; Phosphoprotein; Protein transport; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..118
FT                   /note="BET1 homolog"
FT                   /id="PRO_0000206886"
FT   TOPO_DOM        1..94
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        116..118
FT                   /note="Vesicular"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          26..88
FT                   /note="t-SNARE coiled-coil homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT   MOD_RES         50
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
SQ   SEQUENCE   118 AA;  13230 MW;  9ED4863140DAF051 CRC64;
     MRRAGLGDGA PPGGYGNYGY ANSGYNACEE ENDRLTESLR SKVTAIKSLS IEIGHEVKNQ
     NKLLAEMDSQ FDSTTGFLGK TMGRLKILSR GSQTKLLCYM MLFSLFVFFV IYWIIKLR