S6A20_RAT
ID S6A20_RAT Reviewed; 616 AA.
AC Q64093;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Sodium- and chloride-dependent transporter XTRP3;
DE AltName: Full=Sodium/imino-acid transporter 1;
DE AltName: Full=Solute carrier family 6 member 20;
DE AltName: Full=Transporter rB21A;
GN Name=Slc6a20; Synonyms=Sit1, Xtrp3;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=8001687; DOI=10.1016/0014-5793(94)01328-x;
RA Smith K.E., Fried S.G., Durkin M.M., Gustafson E.L., Borden L.A.,
RA Branchek T.A., Weinshank R.L.;
RT "Molecular cloning of an orphan transporter. A new member of the
RT neurotransmitter transporter family.";
RL FEBS Lett. 357:86-92(1995).
RN [2]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15632147; DOI=10.1074/jbc.m413027200;
RA Takanaga H., Mackenzie B., Suzuki Y., Hediger M.A.;
RT "Identification of mammalian proline transporter SIT1 (SLC6A20) with
RT characteristics of classical system imino.";
RL J. Biol. Chem. 280:8974-8984(2005).
CC -!- FUNCTION: Mediates the calcium-dependent uptake of imino acids such as
CC L-proline, N-methyl-L-proline and pipecolate as well as N-methylated
CC amino acids. {ECO:0000269|PubMed:15632147}.
CC -!- SUBCELLULAR LOCATION: Apical cell membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Located in the apical brush border
CC membrane of kidney proximal tubule cells. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highly expressed in epithelial cells of duodenum,
CC jejunum, ileum, stomach, cecum, colon and kidney proximal tubule. Also
CC expressed in the choroid plexus, microglia and meniges of the brain and
CC in the ovary. {ECO:0000269|PubMed:15632147}.
CC -!- SIMILARITY: Belongs to the sodium:neurotransmitter symporter (SNF) (TC
CC 2.A.22) family. SLC6A20 subfamily. {ECO:0000305}.
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DR EMBL; S76742; AAB32806.1; -; mRNA.
DR PIR; S50998; S50998.
DR RefSeq; NP_579830.1; NM_133296.1.
DR AlphaFoldDB; Q64093; -.
DR SMR; Q64093; -.
DR STRING; 10116.ENSRNOP00000007467; -.
DR TCDB; 2.A.22.6.1; the neurotransmitter:sodium symporter (nss) family.
DR GlyGen; Q64093; 2 sites.
DR PaxDb; Q64093; -.
DR GeneID; 113918; -.
DR KEGG; rno:113918; -.
DR UCSC; RGD:621651; rat.
DR CTD; 102680; -.
DR RGD; 621651; Slc6a20.
DR eggNOG; KOG3659; Eukaryota.
DR InParanoid; Q64093; -.
DR OrthoDB; 547281at2759; -.
DR PhylomeDB; Q64093; -.
DR Reactome; R-RNO-352230; Amino acid transport across the plasma membrane.
DR Reactome; R-RNO-442660; Na+/Cl- dependent neurotransmitter transporters.
DR PRO; PR:Q64093; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0015199; F:amino-acid betaine transmembrane transporter activity; IMP:ARUK-UCL.
DR GO; GO:0015193; F:L-proline transmembrane transporter activity; IDA:ARUK-UCL.
DR GO; GO:0015175; F:neutral amino acid transmembrane transporter activity; ISO:RGD.
DR GO; GO:0005298; F:proline:sodium symporter activity; IDA:ARUK-UCL.
DR GO; GO:0089718; P:amino acid import across plasma membrane; IDA:ARUK-UCL.
DR GO; GO:0006865; P:amino acid transport; IDA:UniProtKB.
DR GO; GO:0015838; P:amino-acid betaine transport; IMP:ARUK-UCL.
DR GO; GO:0015816; P:glycine transport; ISO:RGD.
DR GO; GO:1904271; P:L-proline import across plasma membrane; IDA:ARUK-UCL.
DR GO; GO:1905647; P:proline import across plasma membrane; ISO:RGD.
DR GO; GO:0035524; P:proline transmembrane transport; ISO:RGD.
DR GO; GO:0015824; P:proline transport; ISO:RGD.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000175; Na/ntran_symport.
DR InterPro; IPR002438; Neutral_aa_SLC6.
DR InterPro; IPR037272; SNS_sf.
DR PANTHER; PTHR11616; PTHR11616; 1.
DR Pfam; PF00209; SNF; 1.
DR PRINTS; PR00176; NANEUSMPORT.
DR PRINTS; PR01206; ORPHTRNSPORT.
DR SUPFAM; SSF161070; SSF161070; 1.
DR PROSITE; PS00610; NA_NEUROTRAN_SYMP_1; 1.
DR PROSITE; PS00754; NA_NEUROTRAN_SYMP_2; 1.
DR PROSITE; PS50267; NA_NEUROTRAN_SYMP_3; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..616
FT /note="Sodium- and chloride-dependent transporter XTRP3"
FT /id="PRO_0000214814"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..66
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..103
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..265
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..413
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 435..455
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 456..476
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 477..489
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 490..510
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 511..533
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 534..554
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 555..578
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 579..599
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 600..616
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 155
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 381
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 616 AA; 68971 MW; 6B0DAB04315CE829 CRC64;
MRLAIKRRAS RGQRPGPDEK RARDMEKARP QWGNPLQFVF ACISYAVGLG NVWRFPYLCQ
MYGGGSFLVP YLIMLIVEGM PLLYLELAVG QRMRQGSIGA WRTISPYLSG VGVASVVVSF
FLSMYYNVIN AWGFWYLFHS FQDPLPWSVC PLNSNRTGYD EECEKASSTQ YFWYRKTLNI
SPSIQENGGV QWEPALCLTL AWLMVYLCIL RGTESTGKVV YFTALMPYCV LIIYLVRGLT
LHGATNGLMY MFTPKIEQLA NPKAWINAAT QIFFSLGLGF GSLIAFASYN EPSNDCQKHA
VIVSVINSST SIFASIVTFS IYGFKATFNY ENCLNKVILL LTNSFDLEDG FLTASNLEEV
KDYLASTYPN KYSEVFPHIR NCSLESELNT AVQGTGLAFI VYAEAIKNME VSQLWSVLYF
FMLLMLGMGS MLGNTAAILT PLTDSKVISS YLPKEAISGL VCLINCAVGM VFTMEAGNYW
FDIFNDYAAT LSLLLIVLVE TIAVCYVYGL RRFESDLRAM TGRPLNWYWK AMWAFVSPLL
IIGLFIFYLS DYILTGTLQY QAWDATQGQL VTKDYPPHAL AVIGLLVASS TMCIPLVALG
TFIRNRLKRG GSSPVA
