S7A13_HUMAN
ID S7A13_HUMAN Reviewed; 470 AA.
AC Q8TCU3; Q05C37; Q08AH9; Q96N84;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Solute carrier family 7 member 13;
DE AltName: Full=Sodium-independent aspartate/glutamate transporter 1;
DE AltName: Full=X-amino acid transporter 2;
GN Name=SLC7A13; Synonyms=AGT1, XAT2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC TISSUE=Kidney;
RX PubMed=11943479; DOI=10.1016/s0378-1119(02)00435-3;
RA Blondeau J.-P.;
RT "Homologues of amino acid permeases: cloning and tissue expression of XAT1
RT and XAT2.";
RL Gene 286:241-248(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT MET-249.
RC TISSUE=Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP MET-249.
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Mediates the transport L-aspartate and L-glutamate in a
CC sodium-independent manner. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8TCU3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TCU3-2; Sequence=VSP_033067, VSP_033068, VSP_033069;
CC -!- TISSUE SPECIFICITY: Expressed in the kidney.
CC {ECO:0000269|PubMed:11943479}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. {ECO:0000305}.
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DR EMBL; AJ417661; CAD10393.1; -; mRNA.
DR EMBL; AK055815; BAB71021.1; -; mRNA.
DR EMBL; CH471060; EAW91633.1; -; Genomic_DNA.
DR EMBL; BC125165; AAI25166.1; -; mRNA.
DR EMBL; BC125166; AAI25167.1; -; mRNA.
DR EMBL; BC029436; AAH29436.1; ALT_TERM; mRNA.
DR CCDS; CCDS34917.1; -. [Q8TCU3-1]
DR RefSeq; NP_620172.2; NM_138817.2. [Q8TCU3-1]
DR AlphaFoldDB; Q8TCU3; -.
DR SMR; Q8TCU3; -.
DR BioGRID; 127618; 9.
DR IntAct; Q8TCU3; 2.
DR MINT; Q8TCU3; -.
DR STRING; 9606.ENSP00000297524; -.
DR TCDB; 2.A.3.8.24; the amino acid-polyamine-organocation (apc) family.
DR iPTMnet; Q8TCU3; -.
DR PhosphoSitePlus; Q8TCU3; -.
DR BioMuta; SLC7A13; -.
DR DMDM; 74751412; -.
DR PaxDb; Q8TCU3; -.
DR PeptideAtlas; Q8TCU3; -.
DR PRIDE; Q8TCU3; -.
DR Antibodypedia; 54555; 5 antibodies from 4 providers.
DR DNASU; 157724; -.
DR Ensembl; ENST00000297524.8; ENSP00000297524.3; ENSG00000164893.9. [Q8TCU3-1]
DR Ensembl; ENST00000419776.2; ENSP00000410982.2; ENSG00000164893.9. [Q8TCU3-2]
DR GeneID; 157724; -.
DR KEGG; hsa:157724; -.
DR MANE-Select; ENST00000297524.8; ENSP00000297524.3; NM_138817.3; NP_620172.2.
DR UCSC; uc003ydq.2; human. [Q8TCU3-1]
DR CTD; 157724; -.
DR DisGeNET; 157724; -.
DR GeneCards; SLC7A13; -.
DR HGNC; HGNC:23092; SLC7A13.
DR HPA; ENSG00000164893; Tissue enriched (kidney).
DR MIM; 617256; gene.
DR neXtProt; NX_Q8TCU3; -.
DR OpenTargets; ENSG00000164893; -.
DR PharmGKB; PA134980828; -.
DR VEuPathDB; HostDB:ENSG00000164893; -.
DR eggNOG; KOG1287; Eukaryota.
DR GeneTree; ENSGT00940000162798; -.
DR HOGENOM; CLU_007946_3_0_1; -.
DR InParanoid; Q8TCU3; -.
DR OMA; SPNVHYV; -.
DR OrthoDB; 621852at2759; -.
DR PhylomeDB; Q8TCU3; -.
DR TreeFam; TF313355; -.
DR PathwayCommons; Q8TCU3; -.
DR SignaLink; Q8TCU3; -.
DR BioGRID-ORCS; 157724; 9 hits in 1066 CRISPR screens.
DR ChiTaRS; SLC7A13; human.
DR GenomeRNAi; 157724; -.
DR Pharos; Q8TCU3; Tdark.
DR PRO; PR:Q8TCU3; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q8TCU3; protein.
DR Bgee; ENSG00000164893; Expressed in kidney epithelium and 36 other tissues.
DR Genevisible; Q8TCU3; HS.
DR GO; GO:0016324; C:apical plasma membrane; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IEA:Ensembl.
DR GO; GO:0015810; P:aspartate transmembrane transport; IEA:Ensembl.
DR GO; GO:0015811; P:L-cystine transport; IEA:Ensembl.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; IEA:Ensembl.
DR InterPro; IPR002293; AA/rel_permease1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..470
FT /note="Solute carrier family 7 member 13"
FT /id="PRO_0000330725"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..45
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109..128
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..149
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..162
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..206
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..287
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..336
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 380..393
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..421
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 443..470
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 221..229
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033067"
FT VAR_SEQ 394..442
FT /note="VFLSFPLATIVIDVGLVVIPLVKSPNVHYVYVLLLVLSGLLFYIPLIHF ->
FT SLILVVQAGEAGVQWHDLGLLQPLLPGFKRFSCLSLPSSWDYRCFCHFH (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033068"
FT VAR_SEQ 443..470
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033069"
FT VARIANT 249
FT /note="V -> M (in dbSNP:rs2976189)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_042716"
FT VARIANT 380
FT /note="R -> K (in dbSNP:rs4419794)"
FT /id="VAR_042717"
FT VARIANT 452
FT /note="M -> T (in dbSNP:rs9656982)"
FT /id="VAR_042718"
FT VARIANT 470
FT /note="E -> K (in dbSNP:rs9693999)"
FT /id="VAR_042719"
SQ SEQUENCE 470 AA; 52114 MW; F8593B829E2050E7 CRC64;
MDRGEKIQLK RVFGYWWGTS FLLINIIGAG IFVSPKGVLA YSCMNVGVSL CVWAGCAILA
MTSTLCSAEI SISFPCSGAQ YYFLKRYFGS TVAFLNLWTS LFLGSGVVAG QALLLAEYSI
QPFFPSCSVP KLPKKCLALA MLWIVGILTS RGVKEVTWLQ IASSVLKVSI LSFISLTGVV
FLIRGKKENV ERFQNAFDAE LPDISHLIQA IFQGYFAYSG GACFTLIAGE LKKPRTTIPK
CIFTALPLVT VVYLLVNISY LTVLTPREIL SSDAVAITWA DRAFPSLAWI MPFAISTSLF
SNLLISIFKS SRPIYLASQE GQLPLLFNTL NSHSSPFTAV LLLVTLGSLA IILTSLIDLI
NYIFFTGSLW SILLMIGILR RRYQEPNLSI PYKVFLSFPL ATIVIDVGLV VIPLVKSPNV
HYVYVLLLVL SGLLFYIPLI HFKIRLAWFE KMTCYLQLLF NICLPDVSEE
