S7A13_MOUSE
ID S7A13_MOUSE Reviewed; 478 AA.
AC Q91WN3;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Solute carrier family 7 member 13;
DE AltName: Full=Sodium-independent aspartate/glutamate transporter 1;
DE AltName: Full=X-amino acid transporter 2;
GN Name=Slc7a13; Synonyms=Agt1, Xat2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ; TISSUE=Kidney;
RX PubMed=11943479; DOI=10.1016/s0378-1119(02)00435-3;
RA Blondeau J.-P.;
RT "Homologues of amino acid permeases: cloning and tissue expression of XAT1
RT and XAT2.";
RL Gene 286:241-248(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=ICR; TISSUE=Kidney;
RX PubMed=11907033; DOI=10.1074/jbc.m200019200;
RA Matsuo H., Kanai Y., Kim J.Y., Chairoungdua A., Kim D.K., Inatomi J.,
RA Shigeta Y., Ishimine H., Chaekuntode S., Tachampa K., Choi H.W., Babu E.,
RA Fukuda J., Endou H.;
RT "Identification of a novel Na+-independent acidic amino acid transporter
RT with structural similarity to the member of a heterodimeric amino acid
RT transporter family associated with unknown heavy chains.";
RL J. Biol. Chem. 277:21017-21026(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Mediates the transport L-aspartate and L-glutamate in a
CC sodium-independent manner. {ECO:0000269|PubMed:11907033}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the kidney.
CC {ECO:0000269|PubMed:11907033, ECO:0000269|PubMed:11943479}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. {ECO:0000305}.
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DR EMBL; AJ417662; CAD10394.1; -; mRNA.
DR EMBL; AB072352; BAC00494.1; -; mRNA.
DR EMBL; AK143768; BAE25532.1; -; mRNA.
DR EMBL; BX470225; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC014684; AAH14684.1; -; mRNA.
DR CCDS; CCDS17993.1; -.
DR RefSeq; NP_083022.1; NM_028746.3.
DR AlphaFoldDB; Q91WN3; -.
DR SMR; Q91WN3; -.
DR STRING; 10090.ENSMUSP00000036228; -.
DR TCDB; 2.A.3.8.8; the amino acid-polyamine-organocation (apc) family.
DR PhosphoSitePlus; Q91WN3; -.
DR jPOST; Q91WN3; -.
DR PaxDb; Q91WN3; -.
DR PRIDE; Q91WN3; -.
DR ProteomicsDB; 256911; -.
DR Antibodypedia; 54555; 5 antibodies from 4 providers.
DR DNASU; 74087; -.
DR Ensembl; ENSMUST00000035890; ENSMUSP00000036228; ENSMUSG00000041052.
DR GeneID; 74087; -.
DR KEGG; mmu:74087; -.
DR UCSC; uc008scf.2; mouse.
DR CTD; 157724; -.
DR MGI; MGI:1921337; Slc7a13.
DR VEuPathDB; HostDB:ENSMUSG00000041052; -.
DR eggNOG; KOG1287; Eukaryota.
DR GeneTree; ENSGT00940000162798; -.
DR HOGENOM; CLU_007946_3_0_1; -.
DR InParanoid; Q91WN3; -.
DR OMA; SPNVHYV; -.
DR OrthoDB; 621852at2759; -.
DR PhylomeDB; Q91WN3; -.
DR TreeFam; TF313355; -.
DR BioGRID-ORCS; 74087; 4 hits in 72 CRISPR screens.
DR PRO; PR:Q91WN3; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q91WN3; protein.
DR Bgee; ENSMUSG00000041052; Expressed in right kidney and 30 other tissues.
DR Genevisible; Q91WN3; MM.
DR GO; GO:0016324; C:apical plasma membrane; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015179; F:L-amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:MGI.
DR GO; GO:0015810; P:aspartate transmembrane transport; IDA:MGI.
DR GO; GO:0015811; P:L-cystine transport; IDA:MGI.
DR GO; GO:0015813; P:L-glutamate transmembrane transport; IDA:MGI.
DR InterPro; IPR002293; AA/rel_permease1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..478
FT /note="Solute carrier family 7 member 13"
FT /id="PRO_0000330726"
FT TOPO_DOM 1..14
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 15..35
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..47
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 69..89
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..129
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..208
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..242
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 264..288
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 310..338
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 360
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..395
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..423
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 445..478
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 478 AA; 53233 MW; AF9B82699ED66AB8 CRC64;
MAMDSKKEIR LKRELGYFWG TNFLIINIIG AGIFVSPKGV LQHSSMNVGV SLCVWAVCAV
LTLTSALCSA EIGITFPYSG AHYYFLKRCF GPLVAFLRLW TSLFLGPGLI ASQALLLAEY
GVQPFYPSCS APILPRKCLA LAMLWIVGIL NSRGVKELSW LQTVSSVLKV GILGVISLSG
LFLLVRGKKE NVQRLQNAFD AEFPEVSQLI EAIFQGYFAF SGGGCFTCIA GELKKPSKTI
PRCIFTGLPL VTVVYLLANI SYLTVLTPQE MLSSDAVALT WTDRVIPQFT WTVPFAISAS
LFINLVINVL ETSRVLYIAS ENGQLPLLFC ALNVHSSPFI AVLLIISMAS ILIVLTNLID
LINYLYFVVS IWTALSIIGI LKLRYQEPNL HRPYKVFLPF TFIALGITLS LVLIPLVKSP
KLHYIYVFLF LLSGLVFYVP LIHFKVKFVW FQKLTCYLQL LFNICIPDVS DDHIHEES
