BET1_YEAST
ID BET1_YEAST Reviewed; 142 AA.
AC P22804; D6VVS6;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 1.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Protein transport protein BET1;
DE AltName: Full=Suppressor of loss of YPT1 protein 12;
DE Short=Protein SLY12;
GN Name=BET1; Synonyms=SLY12; OrderedLocusNames=YIL004C; ORFNames=YIA4C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1990290; DOI=10.1128/mcb.11.2.872-885.1991;
RA Dascher C., Ossig R., Gallwitz D., Schmitt H.D.;
RT "Identification and structure of four yeast genes (SLY) that are able to
RT suppress the functional loss of YPT1, a member of the RAS superfamily.";
RL Mol. Cell. Biol. 11:872-885(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=1396561; DOI=10.1002/j.1460-2075.1992.tb05445.x;
RA Newman A.P., Groesch M.E., Ferro-Novick S.;
RT "Bos1p, a membrane protein required for ER to Golgi transport in yeast, co-
RT purifies with the carrier vesicles and with Bet1p and the ER membrane.";
RL EMBO J. 11:3609-3617(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7762303; DOI=10.1002/yea.320110109;
RA Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S.,
RA Schwager C., Zimmermann J., Sander C., Ansorge W.;
RT "Nucleotide sequence and analysis of the centromeric region of yeast
RT chromosome IX.";
RL Yeast 11:61-78(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP FUNCTION, AND INTERACTION WITH SED5; BOS1 AND SEC22.
RX PubMed=11001058; DOI=10.1038/35025076;
RA Parlati F., McNew J.A., Fukuda R., Miller R., Sollner T.H., Rothman J.E.;
RT "Topological restriction of SNARE-dependent membrane fusion.";
RL Nature 407:194-198(2000).
RN [7]
RP INTERACTION WITH SEC24.
RX PubMed=12941276; DOI=10.1016/s0092-8674(03)00608-1;
RA Mossessova E., Bickford L.C., Goldberg J.;
RT "SNARE selectivity of the COPII coat.";
RL Cell 114:483-495(2003).
CC -!- FUNCTION: SNARE required for targeting and fusion of ER-derived
CC transport vesicles with the Golgi complex.
CC {ECO:0000269|PubMed:11001058, ECO:0000269|PubMed:1396561}.
CC -!- SUBUNIT: Component of a SNARE complex consisting of SED5, BOS1, BET1
CC and SEC22 or YKT6. Interacts with SEC24. {ECO:0000269|PubMed:11001058,
CC ECO:0000269|PubMed:12941276}.
CC -!- INTERACTION:
CC P22804; P38182: ATG8; NbExp=2; IntAct=EBI-3550, EBI-2684;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000305}; Single-
CC pass type IV membrane protein {ECO:0000305}. Endoplasmic reticulum
CC membrane {ECO:0000305}; Single-pass type IV membrane protein
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the BET1 family. {ECO:0000305}.
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DR EMBL; Z38113; CAA86247.1; -; Genomic_DNA.
DR EMBL; X54237; CAA38143.1; -; Genomic_DNA.
DR EMBL; X79743; CAB38096.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08542.1; -; Genomic_DNA.
DR PIR; C39610; C39610.
DR RefSeq; NP_012262.3; NM_001179354.3.
DR PDB; 1PCX; X-ray; 2.50 A; B=51-58.
DR PDBsum; 1PCX; -.
DR AlphaFoldDB; P22804; -.
DR SMR; P22804; -.
DR BioGRID; 34988; 280.
DR ComplexPortal; CPX-1854; Golgi SNARE complex SED5-BOS1-BET1-SEC22.
DR DIP; DIP-2050N; -.
DR IntAct; P22804; 8.
DR MINT; P22804; -.
DR STRING; 4932.YIL004C; -.
DR MaxQB; P22804; -.
DR PaxDb; P22804; -.
DR PRIDE; P22804; -.
DR TopDownProteomics; P22804; -.
DR EnsemblFungi; YIL004C_mRNA; YIL004C; YIL004C.
DR GeneID; 854813; -.
DR KEGG; sce:YIL004C; -.
DR SGD; S000001266; BET1.
DR VEuPathDB; FungiDB:YIL004C; -.
DR eggNOG; KOG3385; Eukaryota.
DR GeneTree; ENSGT00940000163414; -.
DR HOGENOM; CLU_086133_1_1_1; -.
DR InParanoid; P22804; -.
DR OMA; ARQGCRW; -.
DR BioCyc; YEAST:G3O-31283-MON; -.
DR Reactome; R-SCE-204005; COPII-mediated vesicle transport.
DR Reactome; R-SCE-6807878; COPI-mediated anterograde transport.
DR Reactome; R-SCE-6811438; Intra-Golgi traffic.
DR EvolutionaryTrace; P22804; -.
DR PRO; PR:P22804; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P22804; protein.
DR GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; IDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:1990674; C:Golgi cis cisterna membrane; IC:ComplexPortal.
DR GO; GO:0030173; C:integral component of Golgi membrane; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IDA:SGD.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0031201; C:SNARE complex; IDA:SGD.
DR GO; GO:0005484; F:SNAP receptor activity; IDA:SGD.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IMP:SGD.
DR GO; GO:0006886; P:intracellular protein transport; IDA:ComplexPortal.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IDA:SGD.
DR GO; GO:0006906; P:vesicle fusion; IDA:SGD.
DR GO; GO:0048280; P:vesicle fusion with Golgi apparatus; IDA:ComplexPortal.
DR CDD; cd15853; SNARE_Bet1; 1.
DR InterPro; IPR039897; BET1.
DR InterPro; IPR039899; BET1_SNARE.
DR InterPro; IPR000727; T_SNARE_dom.
DR PANTHER; PTHR12791; PTHR12791; 1.
DR SMART; SM00397; t_SNARE; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Endoplasmic reticulum; ER-Golgi transport;
KW Golgi apparatus; Membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..142
FT /note="Protein transport protein BET1"
FT /id="PRO_0000206892"
FT TOPO_DOM 1..117
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..141
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT DOMAIN 52..114
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 52..56
FT /evidence="ECO:0007829|PDB:1PCX"
SQ SEQUENCE 142 AA; 15720 MW; DC1BD4FFE67E2217 CRC64;
MSSRFAGGNA YQRDTGRTQL FGPADGSNSL DDNVSSALGS TDKLDYSQST LASLESQSEE
QMGAMGQRIK ALKSLSLKMG DEIRGSNQTI DQLGDTFHNT SVKLKRTFGN MMEMARRSGI
SIKTWLIIFF MVGVLFFWVW IT
