ID   S9AD_CAMJU              Reviewed;         258 AA.
AC   Q4VR99;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Spectinomycin 9-adenylyltransferase;
DE   AltName: Full=ORF8 {ECO:0000303|PubMed:15917546};
GN   Name=aad9 {ECO:0000303|PubMed:15917546}; ORFNames=C9J79_09630;
OS   Campylobacter jejuni.
OG   Plasmid pCG8245, and Plasmid pGMI16-002.
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN RESISTANCE TO SPECTINOMYCIN,
RP   AND DISRUPTION PHENOTYPE.
RC   STRAIN=CG8245 / Serotype Lior 19; PLASMID=pCG8245;
RX   PubMed=15917546; DOI=10.1128/aac.49.6.2454-2459.2005;
RA   Nirdnoy W., Mason C.J., Guerry P.;
RT   "Mosaic structure of a multiple-drug-resistant, conjugative plasmid from
RT   Campylobacter jejuni.";
RL   Antimicrob. Agents Chemother. 49:2454-2459(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFSAN054107; PLASMID=pGMI16-002;
RA   Hoffmann M., Pedersen S.K., Sanchez M., Pettengill J.B., Hendriksen R.S.;
RT   "Genome assemblies for Global Microbial Identifier (GMI) Proficiency
RT   Testing reference strains.";
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mediates bacterial resistance to spectinomycin, is probably a
CC       spectinomycin 9-adenylyltransferase. {ECO:0000269|PubMed:15917546}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate;
CC         Xref=Rhea:RHEA:63228, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:146260, ChEBI:CHEBI:146261;
CC         Evidence={ECO:0000305|PubMed:15917546};
CC   -!- DISRUPTION PHENOTYPE: Loss of spectinomycin resistance.
CC       {ECO:0000269|PubMed:15917546}.
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DR   EMBL; AY701528; AAW34145.1; -; Genomic_DNA.
DR   EMBL; CP028186; AVS37844.1; -; Genomic_DNA.
DR   RefSeq; WP_002578722.1; NZ_SSMB01000017.1.
DR   AlphaFoldDB; Q4VR99; -.
DR   SMR; Q4VR99; -.
DR   EnsemblBacteria; AVS37844; AVS37844; C9J79_09630.
DR   GeneID; 60292662; -.
DR   GO; GO:0070566; F:adenylyltransferase activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.460.10; -; 1.
DR   InterPro; IPR024172; AadA/Aad9.
DR   InterPro; IPR025184; DUF4111.
DR   InterPro; IPR043519; NT_sf.
DR   InterPro; IPR041633; Polbeta.
DR   Pfam; PF13427; DUF4111; 1.
DR   Pfam; PF18765; Polbeta; 1.
DR   PIRSF; PIRSF000819; Streptomycin_3-adenylyltransf; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Plasmid; Transferase.
FT   CHAIN           1..258
FT                   /note="Spectinomycin 9-adenylyltransferase"
FT                   /id="PRO_0000450271"
SQ   SEQUENCE   258 AA;  29316 MW;  CEA287F5D0D88C41 CRC64;
     MNINEFPQQV NQVISIAETI LQGQILGIYL YGSATMNGLR PDSDIDILII TKQELSNSIR
     ADLTKQLLKI SGSVGCIEKR PLEVTIINQS DIVPLQFPPK CQYMYGEWLR GEMEAGEYPQ
     ACNDPDIMIL LWQARKNSIT LKGAESKELI PAIPFHEIKK AIRFSLPGLI SSFKGDERNV
     LLTLSRMWFT LVTEEITTKD VAAKWVILKL PERFPPLLTT AKEAYLGNLS DEWETVEKEA
     MALVEYMKKQ IEELLRTE