ID   S9AD_STAAM              Reviewed;         260 AA.
AC   P0A0D0; P04827;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Spectinomycin 9-adenylyltransferase {ECO:0000250|UniProtKB:P0A0D2};
DE   AltName: Full=AAD(9);
GN   Name=ant1; Synonyms=spc1; OrderedLocusNames=SAV0053;
GN   and
GN   Name=ant2; Synonyms=spc2; OrderedLocusNames=SAV1656;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
CC   -!- FUNCTION: Mediates bacterial resistance to the antibiotic spectinomycin
CC       but not streptomycin. {ECO:0000250|UniProtKB:P0A0D2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + spectinomycin = 9-O-adenylylspectinomycin + diphosphate;
CC         Xref=Rhea:RHEA:63228, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:146260, ChEBI:CHEBI:146261;
CC         Evidence={ECO:0000250|UniProtKB:P0A0D2};
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DR   EMBL; BA000017; BAB56215.1; -; Genomic_DNA.
DR   EMBL; BA000017; BAB57818.1; -; Genomic_DNA.
DR   RefSeq; WP_000067268.1; NC_002758.2.
DR   AlphaFoldDB; P0A0D0; -.
DR   SMR; P0A0D0; -.
DR   World-2DPAGE; 0002:P0A0D0; -.
DR   PaxDb; P0A0D0; -.
DR   EnsemblBacteria; BAB56215; BAB56215; SAV0053.
DR   EnsemblBacteria; BAB57818; BAB57818; SAV1656.
DR   GeneID; 42043718; -.
DR   GeneID; 58051002; -.
DR   KEGG; sav:SAV0053; -.
DR   KEGG; sav:SAV1656; -.
DR   HOGENOM; CLU_071584_0_1_9; -.
DR   OMA; VRPWRYP; -.
DR   PhylomeDB; P0A0D0; -.
DR   BioCyc; SAUR158878:SAV_RS00395-MON; -.
DR   BioCyc; SAUR158878:SAV_RS08880-MON; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0070566; F:adenylyltransferase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.460.10; -; 1.
DR   InterPro; IPR024172; AadA/Aad9.
DR   InterPro; IPR025184; DUF4111.
DR   InterPro; IPR043519; NT_sf.
DR   InterPro; IPR002934; Polymerase_NTP_transf_dom.
DR   Pfam; PF13427; DUF4111; 1.
DR   Pfam; PF01909; NTP_transf_2; 1.
DR   PIRSF; PIRSF000819; Streptomycin_3-adenylyltransf; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase; Transposable element.
FT   CHAIN           1..260
FT                   /note="Spectinomycin 9-adenylyltransferase"
FT                   /id="PRO_0000068584"
SQ   SEQUENCE   260 AA;  28975 MW;  3C821F312A108F54 CRC64;
     MSNLINGKIP NQAIQTLKIV KDLFGSSIVG VYLFGSAVNG GLRINSDVDV LVVVNHSLPQ
     LTRKKLTERL MTISGKIGNT DSVRPLEVTV INRSEVVPWQ YPPKREFIYG EWLRGEFENG
     QIQEPSYDPD LAIVLAQARK NSISLFGPDS SSILVSVPLT DIRRAIKDSL PELIEGIKGD
     ERNVILTLAR MWQTVTTGEI TSKDVAAEWA IPLLPKEHVT LLDIARKGYR GECDDKWEGL
     YSKVKALVKY MKNSIETSLN