SAA3_BOVIN
ID SAA3_BOVIN Reviewed; 131 AA.
AC Q8SQ28; A0A023VWT2; Q32PB7; Q56J78; Q9MZT5;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Serum amyloid A-3 protein;
DE Flags: Precursor;
GN Name=SAA3;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Mammary gland;
RX PubMed=11048944; DOI=10.2527/2000.78102756x;
RA Kho Y.J., Cho K.K., Kim S.C., Kim S.H., Chung M.I., Baik M.G., Choi Y.J.;
RT "Rapid communication: cloning of bovine serum amyloid A3 cDNA.";
RL J. Anim. Sci. 78:2756-2757(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-39; 49-57; 66-74; 81-92
RP AND 115-131, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Mammary gland;
RX PubMed=11730930; DOI=10.1016/s0165-2427(01)00380-4;
RA McDonald T.L., Larson M.A., Mack D.R., Weber A.;
RT "Elevated extrahepatic expression and secretion of mammary-associated serum
RT amyloid A 3 (M-SAA3) into colostrum.";
RL Vet. Immunol. Immunopathol. 83:203-211(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Gutierrez-Pabello J.A., Barthel R., Adams G.;
RT "Differential gene expression of Mycobacterium bovis infected bovine
RT macrophages.";
RL Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION BY
RP LPS; LTA AND PRL.
RX PubMed=16837143; DOI=10.1016/j.gene.2006.05.019;
RA Larson M.A., Weber A., McDonald T.L.;
RT "Bovine serum amyloid A3 gene structure and promoter analysis: induced
RT transcriptional expression by bacterial components and the hormone
RT prolactin.";
RL Gene 380:104-110(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] (ALLELE SAA3.2), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Mammary gland;
RX PubMed=19283521; DOI=10.1080/13547500902730714;
RA Molenaar A.J., Harris D.P., Rajan G.H., Pearson M.L., Callaghan M.R.,
RA Sommer L., Farr V.C., Oden K.E., Miles M.C., Petrova R.S., Good L.L.,
RA Singh K., McLaren R.D., Prosser C.G., Kim K.S., Wieliczko R.J., Dines M.H.,
RA Johannessen K.M., Grigor M.R., Davis S.R., Stelwagen K.;
RT "The acute-phase protein serum amyloid A3 is expressed in the bovine
RT mammary gland and plays a role in host defence.";
RL Biomarkers 14:26-37(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ALA-108.
RA Singh G., Bhardwaj B., Verma S.;
RT "Serum amyloid A (SAA3) has a potential to act as an early biomarker in
RT bovine subclinical mastitis.";
RL Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Major acute phase reactant. Apolipoprotein of the HDL complex
CC (By similarity). May have a role in protection of the mammary gland
CC during remodeling and infection (Probable). In vitro exhibits
CC antimicrobial activity against Escherichia coli, Streptococcus uberis
CC and Pseudomonas aeruginosa (PubMed:19283521).
CC {ECO:0000255|RuleBase:RU000539, ECO:0000269|PubMed:19283521,
CC ECO:0000305|PubMed:19283521}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11730930,
CC ECO:0000269|PubMed:19283521}.
CC -!- TISSUE SPECIFICITY: Expressed in the liver (PubMed:19283521). Expressed
CC in mammary epithelial cells (PubMed:11048944, PubMed:16837143,
CC PubMed:19283521). Expressed at high levels in mammary ductal cells and
CC vesicle engorged alveoli, but absent from stromal and connective tissue
CC and leukocytes (PubMed:19283521). Secreted into colostrum and mastitic
CC milk (at protein level) (PubMed:16837143, PubMed:19283521). Low
CC expression levels, if any, in normal milk (at protein level)
CC (PubMed:16837143, PubMed:19283521). {ECO:0000269|PubMed:11048944,
CC ECO:0000269|PubMed:16837143, ECO:0000269|PubMed:19283521}.
CC -!- DEVELOPMENTAL STAGE: Expressed at a moderate level in late pregnancy,
CC at a low level through lactation, induced early in milk stasis, and
CC expressed at high levels in most mammary epithelial cells during mid to
CC late involution and inflammation/mastitis.
CC {ECO:0000269|PubMed:11048944, ECO:0000269|PubMed:19283521}.
CC -!- INDUCTION: Up-regulated by Gram-negative bacterial lipopolysaccharide
CC (LPS) and the Gram-positive bacterial lipoteichoic acid (LTA) in
CC mammary epithelial cells and to a lesser extent by prolactin/PRL.
CC {ECO:0000269|PubMed:16837143}.
CC -!- SIMILARITY: Belongs to the SAA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF77630.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF160867; AAF77630.1; ALT_FRAME; mRNA.
DR EMBL; AF335552; AAM11538.1; -; mRNA.
DR EMBL; AF540564; AAN17326.1; -; mRNA.
DR EMBL; DQ298246; ABC00875.1; -; Genomic_DNA.
DR EMBL; AY943216; AAX57025.1; -; mRNA.
DR EMBL; KJ463389; AHY20046.1; -; mRNA.
DR EMBL; BC108181; AAI08182.1; -; mRNA.
DR RefSeq; NP_001229502.1; NM_001242573.1.
DR RefSeq; NP_851359.2; NM_181016.3.
DR SMR; Q8SQ28; -.
DR PRIDE; Q8SQ28; -.
DR GeneID; 281474; -.
DR GeneID; 618238; -.
DR KEGG; bta:281474; -.
DR CTD; 20210; -.
DR HOGENOM; CLU_129936_0_0_1; -.
DR OMA; NYFRPAG; -.
DR OrthoDB; 1417043at2759; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0034364; C:high-density lipoprotein particle; IEA:UniProtKB-UniRule.
DR GO; GO:0006953; P:acute-phase response; IEA:UniProtKB-UniRule.
DR InterPro; IPR000096; Serum_amyloid_A.
DR Pfam; PF00277; SAA; 1.
DR PIRSF; PIRSF002472; Serum_amyloid_A; 1.
DR PRINTS; PR00306; SERUMAMYLOID.
DR SMART; SM00197; SAA; 1.
DR PROSITE; PS00992; SAA; 1.
PE 1: Evidence at protein level;
KW Acute phase; Direct protein sequencing; HDL; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..131
FT /note="Serum amyloid A-3 protein"
FT /evidence="ECO:0000255"
FT /id="PRO_5015099354"
FT VARIANT 48
FT /note="R -> K (in allele SAA3.2)"
FT /evidence="ECO:0000269|PubMed:19283521"
FT VARIANT 64
FT /note="R -> Q (in allele SAA3.2)"
FT /evidence="ECO:0000269|PubMed:19283521"
FT VARIANT 93
FT /note="G -> C (in allele SAA3.2)"
FT /evidence="ECO:0000269|PubMed:19283521"
FT VARIANT 108
FT /note="F -> A (in allele SAA3.2)"
FT /evidence="ECO:0000269|PubMed:19283521, ECO:0000269|Ref.6"
FT CONFLICT 129
FT /note="D -> N (in Ref. 5; AAX57025)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 131 AA; 14724 MW; BB5F58F07A3B9331 CRC64;
MNLSTGIIFC FLILGVSSQR WGTFLKEAGQ GAKDMWRAYQ DMKEANYRGA DKYFHARGNY
DAARRGPGGA WAAKVISNAR ETIQGITDPL FKGMTRDQVR EDSKADQFAN EWGRSGKDPN
HFRPAGLPDK Y
