ID   SAC1_CHLRE              Reviewed;         585 AA.
AC   Q39593;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Putative sulfur deprivation response regulator;
GN   Name=SAC1;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=137c / CC-125;
RX   PubMed=8641280; DOI=10.1002/j.1460-2075.1996.tb00568.x;
RA   Davies J.P., Yildiz F.H., Grossman A.;
RT   "Sac1, a putative regulator that is critical for survival of Chlamydomonas
RT   reinhardtii during sulfur deprivation.";
RL   EMBO J. 15:2150-2159(1996).
CC   -!- FUNCTION: Not known; mutations in SAC1 produces cells that cannot
CC       synthesize arylsulfatase and cannot take up sulfate as rapidly as wild-
CC       type cells. SAC1 is necessary for cells to survive sulfur deprivation.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CitM (TC 2.A.11) transporter family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U47541; AAB08008.1; -; mRNA.
DR   PIR; S69216; S69216.
DR   AlphaFoldDB; Q39593; -.
DR   STRING; 3055.EDP00156; -.
DR   eggNOG; ENOG502QPZM; Eukaryota.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008324; F:cation transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0006813; P:potassium ion transport; IEA:InterPro.
DR   Gene3D; 3.30.70.1450; -; 2.
DR   InterPro; IPR004680; Cit_transptr-like_dom.
DR   InterPro; IPR031312; Na/sul_symport_CS.
DR   InterPro; IPR006037; RCK_C.
DR   InterPro; IPR036721; RCK_C_sf.
DR   Pfam; PF03600; CitMHS; 2.
DR   Pfam; PF02080; TrkA_C; 1.
DR   SUPFAM; SSF116726; SSF116726; 2.
DR   PROSITE; PS01271; NA_SULFATE; 1.
DR   PROSITE; PS51202; RCK_C; 2.
PE   2: Evidence at transcript level;
KW   Membrane; Repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..585
FT                   /note="Putative sulfur deprivation response regulator"
FT                   /id="PRO_0000172508"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        162..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        389..409
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        411..431
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        442..462
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        482..502
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        561..581
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          189..274
FT                   /note="RCK C-terminal 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
FT   DOMAIN          288..372
FT                   /note="RCK C-terminal 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
SQ   SEQUENCE   585 AA;  62329 MW;  0603B05D86BD81C3 CRC64;
     MIRGVVDADV CLFAASTLLL LRGIIQARDA FAGLANDSIV SIALMMMIAA GLESSGALEF
     VPELVLGRSK REWVGQLRMH IAVASVSAVM NNTPLVAVMI PVVESWCRNN NHHPSRFMMP
     LSYSAILGGL CTIIGTSTNL IARGLAQQDD PKLKLPFVEV GIIGLPLTVA GGIYVVLFSP
     LLLRKRDTMM AAVVADPREY VVSVRVDARF AHIGRTIESA GLRHLRGLFL ADLQRQDGAT
     VPSPPPTTII LQGDKLTFAG DIQGMQHILS LPGLTPISSA DLAADLEETV AGSPSSDRIM
     VEAVVSLSSP ICNMTIRDSH FRSRYGAVVL RVHRNGERIA GGLGDIVVKG GDTMLLEAGP
     DFLQKYKHST EWALAVDAFR VTLPRRDPLA LFMSLGIFIA LIVLNSMDVL PLSTTALVCL
     FAYLITGVLT VSQCRAAIPS SILLTVAGGF GVAKAMTVTG LAHRLAGSLL NVFSWMGRAG
     PVAAIYASTS LLTALLSNGA AVTLMYPIAR DLAKQAGVSI KGPLYALMIG ASSDFSTPIG
     YQTNLMVSGP GGYRFLDFTR FGLPLQFVAA LITVPICVLY FEPRT