SAC3A_ARATH
ID SAC3A_ARATH Reviewed; 1006 AA.
AC F4IUY8; O80947; Q56W50;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=SAC3 family protein A {ECO:0000303|PubMed:19843313};
GN Name=SAC3A {ECO:0000303|PubMed:19843313};
GN OrderedLocusNames=At2g39340 {ECO:0000312|Araport:AT2G39340};
GN ORFNames=T16B24.2 {ECO:0000312|EMBL:AAC28976.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 509-1006.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP INTERACTION WITH EER5; SAC3B AND CML20, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND FUNCTION.
RX PubMed=19843313; DOI=10.1111/j.1365-313x.2009.04048.x;
RA Lu Q., Tang X., Tian G., Wang F., Liu K., Nguyen V., Kohalmi S.E.,
RA Keller W.A., Tsang E.W., Harada J.J., Rothstein S.J., Cui Y.;
RT "Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components
RT and anchoring nucleoporin.";
RL Plant J. 61:259-270(2010).
CC -!- FUNCTION: Component of the TREX-2 complex (transcription and export
CC complex 2), a muliprotein complex that functions in docking export-
CC competent ribonucleoprotein particles (mRNPs) to the nuclear entrance
CC of the nuclear pore complex (nuclear basket). TREX-2 participates in
CC mRNA export and accurate chromatin positioning in the nucleus by
CC tethering genes to the nuclear periphery (PubMed:19843313).
CC {ECO:0000269|PubMed:19843313}.
CC -!- SUBUNIT: Interacts with EER5, SAC3B and CML20.
CC {ECO:0000269|PubMed:19843313}.
CC -!- INTERACTION:
CC F4IUY8; Q8GWE6: EER5; NbExp=3; IntAct=EBI-2619722, EBI-2619706;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19843313}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Sac3a, sac3b and sac3c
CC triple mutants show no visible phenotype.
CC {ECO:0000269|PubMed:19843313}.
CC -!- SIMILARITY: Belongs to the SAC3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC28976.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004697; AAC28976.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09663.1; -; Genomic_DNA.
DR EMBL; AK222197; BAD95343.1; -; mRNA.
DR PIR; T02568; T02568.
DR RefSeq; NP_181466.2; NM_129491.5.
DR AlphaFoldDB; F4IUY8; -.
DR SMR; F4IUY8; -.
DR IntAct; F4IUY8; 3.
DR STRING; 3702.AT2G39340.1; -.
DR PaxDb; F4IUY8; -.
DR PRIDE; F4IUY8; -.
DR ProteomicsDB; 232683; -.
DR EnsemblPlants; AT2G39340.1; AT2G39340.1; AT2G39340.
DR GeneID; 818519; -.
DR Gramene; AT2G39340.1; AT2G39340.1; AT2G39340.
DR KEGG; ath:AT2G39340; -.
DR Araport; AT2G39340; -.
DR TAIR; locus:2056058; AT2G39340.
DR eggNOG; KOG1861; Eukaryota.
DR HOGENOM; CLU_015513_3_0_1; -.
DR InParanoid; F4IUY8; -.
DR OMA; QYVNQAP; -.
DR OrthoDB; 152596at2759; -.
DR PRO; PR:F4IUY8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IUY8; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0000380; P:alternative mRNA splicing, via spliceosome; IMP:TAIR.
DR GO; GO:0008380; P:RNA splicing; IMP:TAIR.
DR InterPro; IPR000717; PCI_dom.
DR InterPro; IPR045107; SAC3/GANP/THP3.
DR InterPro; IPR005062; SAC3/GANP/THP3_conserved.
DR PANTHER; PTHR12436; PTHR12436; 1.
DR Pfam; PF03399; SAC3_GANP; 1.
DR PROSITE; PS50250; PCI; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..1006
FT /note="SAC3 family protein A"
FT /id="PRO_0000435403"
FT DOMAIN 804..978
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT REGION 1..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 106..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 183..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 266..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 516..550
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 595..638
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 650..690
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..75
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 516..541
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 652..675
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 676..690
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 785
FT /note="N -> D (in Ref. 3; BAD95343)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1006 AA; 111260 MW; FD456DDA65DEE5E5 CRC64;
MNHGGNTQAV APMDPNSIEN RYGVDGSQTQ KYSYQYSTGS ESAPWTGHSV ENQAVENGNY
SNSNYYHPQP TGPATGNVQE IPNTVSFTIS STSGTANVAQ DYSGYTPYQT SSDPHNYSNT
GYSNYYSGYQ QQPSQSYPQP VGAYQNTGAP QPLSSFQNPG SYAGTPSYSG TYYNPADYQT
AGGYQSTNYN NQTAGSYPST NYSNQTPASN QGNYTDYTSN PYQNYTPDAA NTHSSTIATT
PPVHYQQNYQ QWTEYYSQTE VPCAPGTEKL STPTTSAYSQ SFPVPGVTSE MPASNSQPAP
SYVQPWRPET DSSHPPSQQP GAAVSTSNDT YWMHQAPSLQ AHHPVPPQNN YQSPLETKPL
YETPFQGHQR ATYPQEMNSQ SSFHQAPLGY RQPTQTAPLV DSQRVSKVQI PTNPRIASNL
PSGFTKMDKD STAASAAQAP AYVSVSMPKP KDHTTAMSDP GTFPKSLRGF VERAFARCKD
DKEKESCEVA LRKIVKKAKE DNTLYTRDWD TEPLSTVTTT NVTNSESSSA QLSSLQNKSP
TRRPKSRWEP LVEGKPFVKP ASTFSSAVKF GVWNHQNENN KKSSESFQKV DAATGFKPTY
SGQNSAKKSF QRPVKRQRFS GGAATAIDDE ASSDSDKDLT PYYSSAMALA GSAEEKKRRD
SRSKRFEKIQ GHSRGNDLTK PKNANVGNLH SRRATALRLS KVFDESGSRA VEDIDWDALT
VKGTCQEIEK RYLRLTSAPD PATVRPEDVL EKALIMVQDS QKNYLFKCDQ LKSIRQDLTV
QRIHNHLTAK VYETHARLAL EAGDLPEYNQ CLSQLKTLYA EGVEGCSLEF AAYSLLYITL
HSNNNRELLS SMSRLSEEDK KDEAVRHALS VRAAVTSGNY VMFFRLYKTA PNMNSCLMDL
YVEKMRYKAV NFMSRSCRPT IPVSYIVQVL GFTGAASEGT DEKETDGMED CLEWLKTHGA
NIITDSNGDM LLDTKATSTS LFMPEPEDAV AHGDRNLDVN DFFTRT
