SAC3B_ARATH
ID SAC3B_ARATH Reviewed; 1697 AA.
AC F4JAU2; Q9M8I9;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=SAC3 family protein B {ECO:0000303|PubMed:19843313};
GN Name=SAC3B {ECO:0000303|PubMed:19843313};
GN OrderedLocusNames=At3g06290 {ECO:0000312|Araport:AT3G06290};
GN ORFNames=F28L1.23 {ECO:0000312|EMBL:AAF30322.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia {ECO:0000312|Proteomes:UP000006548};
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP INTERACTION WITH EER5; SAC3A AND CML19, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND FUNCTION.
RX PubMed=19843313; DOI=10.1111/j.1365-313x.2009.04048.x;
RA Lu Q., Tang X., Tian G., Wang F., Liu K., Nguyen V., Kohalmi S.E.,
RA Keller W.A., Tsang E.W., Harada J.J., Rothstein S.J., Cui Y.;
RT "Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components
RT and anchoring nucleoporin.";
RL Plant J. 61:259-270(2010).
RN [4]
RP INTERACTION WITH UCH1 AND UCH2.
RX PubMed=22951400; DOI=10.4161/psb.21899;
RA Tian G., Lu Q., Kohalmi S.E., Rothstein S.J., Cui Y.;
RT "Evidence that the Arabidopsis Ubiquitin C-terminal Hydrolases 1 and 2
RT associate with the 26S proteasome and the TREX-2 complex.";
RL Plant Signal. Behav. 7:1415-1419(2012).
CC -!- FUNCTION: Component of the TREX-2 complex (transcription and export
CC complex 2), a muliprotein complex that functions in docking export-
CC competent ribonucleoprotein particles (mRNPs) to the nuclear entrance
CC of the nuclear pore complex (nuclear basket). TREX-2 participates in
CC mRNA export and accurate chromatin positioning in the nucleus by
CC tethering genes to the nuclear periphery (PubMed:19843313).
CC {ECO:0000269|PubMed:19843313}.
CC -!- SUBUNIT: Interacts with SAC3A, EER5 and CML19 (PubMed:19843313).
CC Interacts with UCH1 and UCH2 (PubMed:22951400).
CC {ECO:0000269|PubMed:19843313, ECO:0000269|PubMed:22951400}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19843313}.
CC Note=Apparent concentration to the nuclear periphery.
CC {ECO:0000269|PubMed:19843313}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Sac3a, sac3b and sac3c
CC triple mutants show no visible phenotype.
CC {ECO:0000269|PubMed:19843313}.
CC -!- SIMILARITY: Belongs to the SAC3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF30322.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC018907; AAF30322.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74369.1; -; Genomic_DNA.
DR RefSeq; NP_187280.3; NM_111504.3.
DR AlphaFoldDB; F4JAU2; -.
DR SMR; F4JAU2; -.
DR IntAct; F4JAU2; 4.
DR STRING; 3702.AT3G06290.1; -.
DR iPTMnet; F4JAU2; -.
DR PaxDb; F4JAU2; -.
DR PRIDE; F4JAU2; -.
DR ProteomicsDB; 232835; -.
DR EnsemblPlants; AT3G06290.1; AT3G06290.1; AT3G06290.
DR GeneID; 819803; -.
DR Gramene; AT3G06290.1; AT3G06290.1; AT3G06290.
DR KEGG; ath:AT3G06290; -.
DR Araport; AT3G06290; -.
DR TAIR; locus:2082485; AT3G06290.
DR eggNOG; KOG1860; Eukaryota.
DR HOGENOM; CLU_003928_0_0_1; -.
DR InParanoid; F4JAU2; -.
DR OrthoDB; 1593971at2759; -.
DR PRO; PR:F4JAU2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JAU2; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0034399; C:nuclear periphery; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0070390; C:transcription export complex 2; IBA:GO_Central.
DR GO; GO:0006406; P:mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0044030; P:regulation of DNA methylation; IMP:TAIR.
DR InterPro; IPR031907; MCM3AP_GANP.
DR InterPro; IPR000717; PCI_dom.
DR InterPro; IPR045107; SAC3/GANP/THP3.
DR InterPro; IPR005062; SAC3/GANP/THP3_conserved.
DR PANTHER; PTHR12436; PTHR12436; 1.
DR Pfam; PF16769; MCM3AP_GANP; 1.
DR Pfam; PF03399; SAC3_GANP; 1.
DR PROSITE; PS50250; PCI; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..1697
FT /note="SAC3 family protein B"
FT /id="PRO_0000435404"
FT DOMAIN 625..813
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT REGION 54..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 167..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 306..413
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 491..512
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 81..95
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..132
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..280
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..383
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1697 AA; 189932 MW; C70B382BE22E7184 CRC64;
MAFRPFGKDL GPMSSKPAPF TPFGASSTTR LYLSFLFLHT ANVGFACSDS SIQPPASQNH
SAFAGQSFGP GGIRSGPSIQ RAPPLSASQN PQLSIGKPYR PGGVQSVPPI NRIPSPSAFQ
NPSPSSGQPY QPGGIQRIPE PFNGIAWGPE ASRTSPSVRP YQFPGVQRPN LNPQYGHDGS
RNFLKDHGEH SRATSPPATS HILSRMGTDA VEIGRSQDSK RKSRSDILPD QNMGFSRRNQ
SPVSGFENGN LVDGFQPLSS RTWMRSPSSA ENNPVRSRSN PNQLIHQEQT GNSSFPYAHE
VAEIQEATRR KSSAVAPSDK PLGDDPILSQ HDSQRFSTSP PTSGTKSYTL SRSSDSQFPG
QPSSVNSFNN ARKTNSSPAT KRTRSPPVYP IEEDIPRNSF PSQDCTEGEE QARAKRLARF
KGELEPIADR PVDIQLTKSP VNKTMKPLDN KQTFNSLESS RDALKGDALP DYENSEQPSL
IIGVCPDMCP ESERGERERK GDLDHYERVD GDRNQTSKSL AVKKYTRTAE REAILIRPMP
ILQNTMEYLL SLLDRPYNEN FLGMYNFLWD RMRAIRMDLR MQHIFNQEAI TLLEQMIRLH
IIAMHELCEY TKGEGFSEGF DAHLNIEQMN KTSVELFQMY DDHRKKGITV PTEKEFRGYY
ALLKLDKHPG YKVEPSELSL DLANMTPEIR QTSEVLFARN VARACRTGNF IAFFRLARKA
SYLQACLMHA HFSKLRTQAL ASLHSGLQIN QGLPVSDMSN WIGMEEEDIE ALLEYHGFSI
KVFEEPYMVK NDLFLHADKD YKTKCSKLVH MKKSRTIVED VSAPTVVEDV STPFPLPSLI
TEATIGNQQC ITAHKHEMPP ARSLKKQTSM RLFDKEVADS KTSLLAEEDK PMGTFVMNPP
GPFVINPVVH QEKQNDLTSA GGFHSPVKLY SPFGSPKFPQ TKSSNLEKQP NDDRIGMSPG
EIKFSIIGDV YTNHVPGPAL QQSPKSMPME IMPVTTIAEC PTSVENKYAL EESVPEAAMI
CTLEKEFNDI DEEDEDEDGV ILNQYDEEVA KAKLKLIIRL WKRWSSRQSE LRERRQLAAA
AALNSLSLGT PIRFSKTDQS RACGEFNIDQ AMRRRFEERE KSWSRLNISD VIADILVGRN
PESKCISWKV VLCTQTKSVN SSSSASQVTH SAASRWLSSK LMPHAEHSSL NDDNLLFSAP
GVSVWNKWVA NGSDIDFTCC LSVARDVEAE NDMCETTCGA SAVLFLASGG LPLNLQREQL
NLILESVPNG SVLPLLVVIS SCNGEHMEPD TDIVSGLGLH DIDKSKIASF SIVSIANKSQ
KGQEVHFFND SRLRDGFKWL ASNSPLQPNL HHVKLRELFL THFSFSLELL KQMPDQEVGP
NICISAFNDA LETSRRNITS AAEANPIGWP CPETMLLEDN RKECLMVKRY LPNLDWSSAE
NVELLSSVLE NCKLPDFEDD LTWLTVGCAS GAEIENHTQR LEGCLIEYLT QRSNLMGVSL
ATKETGVMLE RNTRLELHNS SRYHITPRWI GIFQRIFNWR IMGLFDASSS SAYVLKSDLN
MSTSSYADKF LAEDASYPSC PPNLPLLHEM IEISCSPLKS PPPYDDKAQR VVETGMLIDD
HRDIEESMLE KNREACRGID LMITEDDELG ERSWRSKGRE AAEKKTIEKR ESERLDELLE
KCNMVQNSIA EKLCIYF
