ID   SACA1_BOVIN             Reviewed;         279 AA.
AC   Q2YDG7;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Sperm acrosome membrane-associated protein 1;
DE   Flags: Precursor;
GN   Name=SPACA1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in acrosome expansion and establishment of
CC       normal sperm morphology during spermatogenesis. Important for male
CC       fertility. {ECO:0000250|UniProtKB:Q9DA48}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC       inner membrane {ECO:0000250|UniProtKB:Q9DA48}; Single-pass type I
CC       membrane protein {ECO:0000305}. Note=Primarily found in the equatorial
CC       segment of the acrosome (By similarity). The tyrosine phosphorylated
CC       protein localizes to a smaller region within the equatorial segment (By
CC       similarity). Also expressed weakly in the principal segment (By
CC       similarity). {ECO:0000250|UniProtKB:D5K8A9,
CC       ECO:0000250|UniProtKB:Q9HBV2}.
CC   -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q9DA48}.
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DR   EMBL; BC110228; AAI10229.1; -; mRNA.
DR   RefSeq; NP_001040010.1; NM_001046545.1.
DR   AlphaFoldDB; Q2YDG7; -.
DR   STRING; 9913.ENSBTAP00000025934; -.
DR   PaxDb; Q2YDG7; -.
DR   GeneID; 614884; -.
DR   KEGG; bta:614884; -.
DR   CTD; 81833; -.
DR   eggNOG; ENOG502S339; Eukaryota.
DR   InParanoid; Q2YDG7; -.
DR   OrthoDB; 1279146at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0002080; C:acrosomal membrane; IBA:GO_Central.
DR   GO; GO:0002079; C:inner acrosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0001675; P:acrosome assembly; IBA:GO_Central.
DR   CDD; cd13783; SPACA1; 1.
DR   InterPro; IPR037878; SPACA1.
DR   PANTHER; PTHR47223; PTHR47223; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Glycoprotein; Membrane; Phosphoprotein;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..279
FT                   /note="Sperm acrosome membrane-associated protein 1"
FT                   /id="PRO_0000248151"
FT   TOPO_DOM        30..221
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        243..279
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          39..74
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          253..279
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        46..68
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        265..279
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         253
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:D5K8A9"
FT   MOD_RES         262
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:D5K8A9"
FT   MOD_RES         275
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9DA48"
FT   CARBOHYD        31
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   279 AA;  30939 MW;  F1ACB2C1882A012F CRC64;
     MKSRGAGCSA RLLLTVGWLL LAGLQSTCGI NVTAIQDPSL AREGEGEPEG DEEPENDSET
     EKEPQAEAED DSEGIGIREV ILTSGCPGGE SKCIVRVEEC RGPVDCGWGR PISESLEHVR
     LACVHTSPEN RFKYIWRLLR PDQQAIILAN DSAILEVHRD THPKAFECET LDNNEIVASI
     RFTIYTTTEL QMKRASRPDT DAVLVFVLTI GVIICIFVIF VLIFIIINWT AVKDFWAKAS
     TTEIQSELSS MRYKDSTSLD QSPTDIPGHE DDALSEWNE